The human immunodeficiency virus type 1 (HIV - 1 ) envelope glycoprotein gp41 plays a critical role in the fusion of viral and target cell membranes. The gp41 extracellular domain,which contains fusion peptide (FP), N - and C - terminal hydrophobic heptad repeats (NHR and CHR, respectively).Peptides derived from NHR and CHR regions,designated N- and C- peptides, respectively, can interact with each other to form a six - stranded coiled - coil domain, representing the fusion-active gp41 core. Our previous studies demonstrated that the C- peptides have potent inhibitory activity against HIV- 1 infection.These peptides inhibit HIV- 1 -mediated membrane fusion by binding to NHR regions for preventing the formation of fusion- active gp41 core.One of the C - peptides, T - 20, which is in the phase Ⅲ clinical trails, is expected to become the first peptide HIV fusion inhibitory drug in the near future.However, this peptide HIV fusion inhibitor lacks oral availability and is sensitive to the proteolytic digestion.Therefore, it is essential to develop small molecular non -peptide HIV fusion inhibitors having similar mechanism of action as the C- peptides.We have established an ELISA- based screening assay using a unique monoclonal antibody, NC- 1, which can specifically bind to a conformational epitope on the gp41 core domain.Using this screening assay, we have identified a small molecular anti- HIV- 1 compound,named ADS-Jl, which inhibits HIV- 1- mediated membrane fusion by blocking the interaction between the NHR and CHR regions to form the fusion - active gp41 core.This compound will be used as a lead to design and develop novel HIV fusion inhibitors as new drugs for the treatment of HIV infection and/or AIDS.

Invasion and metastasis are the important biological features of malignant neoplasms,and are the main reasons for the death of patients with pancreatic neoplasms.Current researches find that invasion and metastasis of pancreatic neoplasms are closely related to series of molecular biological changes,including' abnormalities of tumor metastasis-related genes,degradation of extracellular matrix,tumor-induced angiogenesis,changes of the adhesion molecules and cytokines.However,the definite mechanism still remains unclear,and further researches are needed.

Objective To study the curative effect of edaravone in the treatment of patients with transient cerebral ischemia and its mechanism.Methods 92 patients with TIA treated in our hospital from January 2015 to December 2015 were selected,they were randomly divided into observation group and control group,with 46 cases in each group.Both two groups were given conventional treatment,while the observation group added intravenous infusion of edaravone injection,two groups were treated for 2 weeks.Then,the efficacy and adverse reactions during treatment in two groups were compared.The levels of serum tumor necrosis factor alpha(TNF -α),transforming growth factor beta 1(TGF -β1)and matrix metalloproteinase -9(MMP -9)were detected by enzyme -linked immunosorbent assay(ELISA)in two groups before treatment and 1d,4d,7d,14d after treatment.The levels of high/low shear viscos-ity(HSV /LSV),plasma viscosity(PV),as well as fibrinogen(Fib)were also compared before treatment and 14d after treatment.Results The total effective rate of the observation group was 89.13%,which was significantly higher than 71.74% in the control group(χ2 =4.420,P <0.05).The levels of HSV,LSV,PV and Fib in two groups 14d after treatment were significantly lower than before treatment,which of the observation group were significantly lower than the control group,the differences were statistically significant(P <0.05).The serum levels of TNF -α[(5.99 ± 2.36)pg/mL,(5.82 ±2.34)pg/mL],TGF -β1 [(13.94 ±3.18)pg/L,(12.33 ±3.57)pg/L]and MMP -9 [(42.94 ±11.65)ng/mL,(38.26 ±17.58)ng/mL]in the observation group at 7d and 14d after treatment were sig-nificantly lower than before treatment,the differences were statistically significant (t =4.703,6.031,5.667;5.074, 7.623,6.150;all P <0.05),and which in the observation group were lower than the control group in the same peri-od,the differences were statistically significant(t =2.025,5.344,5.894;2.616,6.120,2.228;all P <0.05).The levels of above indicators 14d after treatment in the control group were significantly lower than before treatment,the differences were statistically significant(P <0.05).There were no serious adverse reactions in the two groups during the treatment.Conclusion Edaravone has obvious curative effect in the treatment of patients with TIA,which works by regulating the hemodynamic and the levels of TNF -α,TGF -β1 and MMP -9,it is safe and worthy of promotion.

The experiments show that extract from Hippocampus trimaculatus Leach (EHT) can inhibit experimental common carotid artery and cerebral thrombosis in rats, and its effective ingredients are unsaturated fatty acids.

The surgical management and the nutritional support in patients with AHNP were reported.We consider that the necrosis of the pancreas complicated with infection should be operated and that parenterally nutritional support could be helpful to patients with AHNP.

Objective To investigate the effect of Astragalus and chemotherapy on the expression of vascular endothelial growth factor(VEGF)in non-small cell lung cancer(NSCLC), microvessel density(MVD)and immune function.Methods 92 patients with NSCLC were divided into the study group and the control group, 46 cases in each group.Both groups were treated with neoadjuvant chemotherapy and operation, and the study group was treated with astragalus polysaccharide.The expression of VEGF in cancer tissues, MVD and immune function were compared between the two groups.The short-term and long-term curative effect and the incidence of adverse reactions were recorded.Results After treatment, positive rates of VEGF, MVD and blood CD8+ were significantly decreased in two groups (P<0.05), showing study groupcontrol group (P<0.05).There were no significant differences between the two groups in short-term curative effect and 5 year survival rates.The incidence rates of side effects in the study group were significantly lower than those in the control group (P<0.05).Conclusion Adjuvant chemotherapy combined with intravenous infusion of Astragalus Polysaccharide can improve MVD, the expression of VEGF and immune function in patients with NSCLC.

Objective To study the inhibitory effects of gene combined radiotherapy on mice transplanted with B16 melanoma.Methods Alkaline lysis assay was used to extract and purify the plasmid.Plasmid DNA was injected into tumor by microinjection assay.Mice were inoculated with 5?10~5 of B16 melanoma in right hind legs and the therapy was performed when the diameter of tumor reached at 0.30.5 cm.pNE-mIL-12 and pcDNA-B7-1 plasmids were injected locally three times following with irradiation three times.The tumor growth rate of mice was observed.Results The anti-tumor effect of pNE-mIL-12 combined with pcDNA-B7-1 plasmid following with 2 Gy X-ray irradiation was much better than other groups.It showed that the tumor growth rate was slowed,the survival days of mice were delayed significantly(P

Objective To study the influence of intraperitoneal instillation of arsenic trioxide (As_2O_3) on metastasis of hepatoma cell line H_22 under CO_2 pneumoperitoneum in mice. Methods The experiment included 40 Kunming mice (cleaning inbred strain). A 1 mm trocar was punctured through the middle abdomen, by which 1?106 tumor cells were administered. Pneumoperitoneum was established with carbon dioxide at the pressure of 8 mm Hg over 30 min. Afterwards, the mice were randomly divided into four groups with 10 mice in each group, receiving intraperitoneal instillation with one of the following: normal saline (1 ml), As_2O_3 (2 mg/kg, 1 ml), As_2O_3 (4 mg/kg, 1 ml), or As_2O_3 (4 mg/kg) with heparin (10 U/ml) (1 ml totally). Levels of CD_44 and vascular endothelial growth factor (VEGF) were measured on the 3rd and 7th day after pneumoperitoneum, respectively. And the survival condition, abdominal circumference, body weight, and diameter of metastatic tumor were compared among the four groups.Results Compared with control group, significantly lower expressions of CD_44 and VEGF were observed the other three groups on the 3rd and 7th day after pneumoperitoneum (P

Retinal neovascularization is a complicated pathophysiological process as a result of imbalance between angiogenic and anti-angiogenic factors. Correct understanding of the signaling pathways,exploring the critical factors involved in retinal angiogenesis, looking for new strategies by reconstructing the new vessels are helpful for knowing the mechanism of the occurrence and development of reitnal neovascularization, which would be good for preventing and treating retinal neovascularization diseases.

Objective: To explore effect of TfR on immune function after ionizing by investigating changes in TfR expression on splenic lymphocytes of mice after whole body irradiation (WBI) with different dose X-rays. Methods: Direct immunoflurescence antibodies and flow cytometry were used to examine the changes of TfR expression. Results: The cell number of TfR positive expression in spleens increased significantly at 24 hours and 72 hours after WBI with 75 mGy X-rays but the cell number of TfR positive expression in spleens decreased significantly at 24 hours after WBI with 1～6 Gy X-rays. The activity of IL-2, meanwhile, demonstrated a parallel change. Conclusion: These results suggest that the TfR enhances immune function in low dose ionizing radiation but suppresses immune function in high dose. The change of TfR expression may be due to the change of IL-2 activity caused by ionizing radiation.