Invasion and metastasis are the important biological features of malignant neoplasms,and are the main reasons for the death of patients with pancreatic neoplasms.Current researches find that invasion and metastasis of pancreatic neoplasms are closely related to series of molecular biological changes,including' abnormalities of tumor metastasis-related genes,degradation of extracellular matrix,tumor-induced angiogenesis,changes of the adhesion molecules and cytokines.However,the definite mechanism still remains unclear,and further researches are needed.

The human immunodeficiency virus type 1 (HIV - 1 ) envelope glycoprotein gp41 plays a critical role in the fusion of viral and target cell membranes. The gp41 extracellular domain,which contains fusion peptide (FP), N - and C - terminal hydrophobic heptad repeats (NHR and CHR, respectively).Peptides derived from NHR and CHR regions,designated N- and C- peptides, respectively, can interact with each other to form a six - stranded coiled - coil domain, representing the fusion-active gp41 core. Our previous studies demonstrated that the C- peptides have potent inhibitory activity against HIV- 1 infection.These peptides inhibit HIV- 1 -mediated membrane fusion by binding to NHR regions for preventing the formation of fusion- active gp41 core.One of the C - peptides, T - 20, which is in the phase Ⅲ clinical trails, is expected to become the first peptide HIV fusion inhibitory drug in the near future.However, this peptide HIV fusion inhibitor lacks oral availability and is sensitive to the proteolytic digestion.Therefore, it is essential to develop small molecular non -peptide HIV fusion inhibitors having similar mechanism of action as the C- peptides.We have established an ELISA- based screening assay using a unique monoclonal antibody, NC- 1, which can specifically bind to a conformational epitope on the gp41 core domain.Using this screening assay, we have identified a small molecular anti- HIV- 1 compound,named ADS-Jl, which inhibits HIV- 1- mediated membrane fusion by blocking the interaction between the NHR and CHR regions to form the fusion - active gp41 core.This compound will be used as a lead to design and develop novel HIV fusion inhibitors as new drugs for the treatment of HIV infection and/or AIDS.

Objective To study the curative effect of edaravone in the treatment of patients with transient cerebral ischemia and its mechanism.Methods 92 patients with TIA treated in our hospital from January 2015 to December 2015 were selected,they were randomly divided into observation group and control group,with 46 cases in each group.Both two groups were given conventional treatment,while the observation group added intravenous infusion of edaravone injection,two groups were treated for 2 weeks.Then,the efficacy and adverse reactions during treatment in two groups were compared.The levels of serum tumor necrosis factor alpha(TNF -α),transforming growth factor beta 1(TGF -β1)and matrix metalloproteinase -9(MMP -9)were detected by enzyme -linked immunosorbent assay(ELISA)in two groups before treatment and 1d,4d,7d,14d after treatment.The levels of high/low shear viscos-ity(HSV /LSV),plasma viscosity(PV),as well as fibrinogen(Fib)were also compared before treatment and 14d after treatment.Results The total effective rate of the observation group was 89.13%,which was significantly higher than 71.74% in the control group(χ2 =4.420,P <0.05).The levels of HSV,LSV,PV and Fib in two groups 14d after treatment were significantly lower than before treatment,which of the observation group were significantly lower than the control group,the differences were statistically significant(P <0.05).The serum levels of TNF -α[(5.99 ± 2.36)pg/mL,(5.82 ±2.34)pg/mL],TGF -β1 [(13.94 ±3.18)pg/L,(12.33 ±3.57)pg/L]and MMP -9 [(42.94 ±11.65)ng/mL,(38.26 ±17.58)ng/mL]in the observation group at 7d and 14d after treatment were sig-nificantly lower than before treatment,the differences were statistically significant (t =4.703,6.031,5.667;5.074, 7.623,6.150;all P <0.05),and which in the observation group were lower than the control group in the same peri-od,the differences were statistically significant(t =2.025,5.344,5.894;2.616,6.120,2.228;all P <0.05).The levels of above indicators 14d after treatment in the control group were significantly lower than before treatment,the differences were statistically significant(P <0.05).There were no serious adverse reactions in the two groups during the treatment.Conclusion Edaravone has obvious curative effect in the treatment of patients with TIA,which works by regulating the hemodynamic and the levels of TNF -α,TGF -β1 and MMP -9,it is safe and worthy of promotion.

Objective To investigate the effect of Astragalus and chemotherapy on the expression of vascular endothelial growth factor(VEGF)in non-small cell lung cancer(NSCLC), microvessel density(MVD)and immune function.Methods 92 patients with NSCLC were divided into the study group and the control group, 46 cases in each group.Both groups were treated with neoadjuvant chemotherapy and operation, and the study group was treated with astragalus polysaccharide.The expression of VEGF in cancer tissues, MVD and immune function were compared between the two groups.The short-term and long-term curative effect and the incidence of adverse reactions were recorded.Results After treatment, positive rates of VEGF, MVD and blood CD8+ were significantly decreased in two groups (P<0.05), showing study groupcontrol group (P<0.05).There were no significant differences between the two groups in short-term curative effect and 5 year survival rates.The incidence rates of side effects in the study group were significantly lower than those in the control group (P<0.05).Conclusion Adjuvant chemotherapy combined with intravenous infusion of Astragalus Polysaccharide can improve MVD, the expression of VEGF and immune function in patients with NSCLC.

The experiments show that extract from Hippocampus trimaculatus Leach (EHT) can inhibit experimental common carotid artery and cerebral thrombosis in rats, and its effective ingredients are unsaturated fatty acids.

The surgical management and the nutritional support in patients with AHNP were reported.We consider that the necrosis of the pancreas complicated with infection should be operated and that parenterally nutritional support could be helpful to patients with AHNP.

Objective To observe the biochemical indicators and lifestyles of patients with non-alcoholic fatty liver disease (NAFLD),and analyse the risk factors of induced NAFLD.Methods A total of 258 in-and out-patients of NAFLD were included in the present study,the control group consisted of 213 examinationers with nonfatty liver disease.All samples were being blood biochemical indicator detection and lifestyle survey.Results Some blood biochemical index of NAFLD patients such as TC(5.48 ± 1.10) mmol/L,TG(2.31 ± 1.25) mmol/L,ALT (51.35 ± 26.18) U/L,AST (42.37 ± 28.32) U/L,FPG (5.62 ± 3.24) mmol/L,GGT (58.47 ± 43.25) U/L and UA (398.51 ± 96.85) μmol/L were higher than those of the control group (t =3.423,5.250,7.402,4.348,3.326,6.683,3.891,all P ＜ 0.01) ; and the incidence of hyperlipidemia,hypertension (21.71％),diabetes (22.09％),BMI(26.85 ± 3.45) or metabolic syndrome (44.57％) etc.Those indexes in NAFLD patients were significantly higher than those in the control group (x2 =8.14,10.55,58.48,t =10.73,all P ＜ 0.01).By multivariable Logistic regression analysis,TG,WI,BMI,HOMA-IR,lack of exercise,high-fat diet were independent risk factors for NAFLD (all P ＜ 0.05).Conclusion Patients with NAFLD is closely correlated to metabolic abnormalities,reasonable diet and a healthy lifestyle is an effective way of prevention and treatment of this disease.

Objective To observe the long-term effect of suramin on the inhibition of proliferation of human retinal pigment epithelial (RPE) cells in vitro. Methods RPE cells grown in 9 pieces of 96-well plate (12 wells each plate) were divided into experimental and control group, with 6 wells in each group. The concentration of 0.1 ml RPE cells in each well is 5?104 cells/ml. After the change of the medium, RPE cells were treated with suramin (250 ?g/ml) in experimental group while treated with nothing in the control group. The medium of the 2 groups were changed to the normal medium after 4 days. At the 1~st , 2~nd , and 4~th day after the addition of suramin and at the 1~st , 2~nd , 3~rd , 5~th , 6~th , 7~th , 9~th , 11~th and 13~th day after removing suramin, 1 plate was randomly selected to stop culturing, and the proliferation of RPE cells were detected by methyl thiazolyl tetrazolium (MTT) assay. Results Under reversed microscope, RPE cells in control group were fused completely at the 7~th day after inoculation. The extracellular space of RPE cells in experimental groups was larger than that in the control group, and remained unfused at the 13~th day after inoculation. The inhibitory rate of proliferation of RPE cells at the first day after treated with suramin was 14.85% and increased to the highest 25.79% at the 4~th day. The first day after the suramin-containing media was removed, the inhibitory rate decreased to 12.35%, and then raised gradually to over 20% at the 3~rd to 5~th day. Finally, the rate drop to 14.71%. Conclusion Suramin has the long-term effect on the inhibition of RPE cells induced by serum, especially the inhibitive effect after the remove of suramin, which indicates the specific double-peak inhibition during the whole process.

Objective To cultivate human retinal capillary endothelial cells (HRECs) and establish two-dimensional model of human retinal vessels in vitro. Methods In a fibronectin-coated raising pound, HRECs were cultured by non-serum human-endothelial-cells substrate and two-dimensional model of human retinal vessels was established. Horseradish peroxidase was used to detect the permeability. Some of the vascular models were cultivated with 5 ng/ml vascular endothelial growth factor (VEGF), whose changes of permeability was compared with which of the models without cultivation with VEGF. The effect of VEGF on vascular permeability was observed. Results Meshy vascular structure came into being due to the confluent HRECs after 2 to 4 days. Comparatively complete two-dimensional vascular model after about 6 days. VEGF increased vascular permeability and promoted the formation of blood vessels. Conclusion HRECs can be cultivated successfully with human-endothelial-cells substrate; standard retinal two-dimensional vascular model in vitro can be established by using cellular raising pound and non-serum human-endothelial-cells substrate.

Purpose To determine the effect of exogenous interleukin 1? (IL 1?) on the retina and its vasculature and VEGF expression in SD rats. Methods IL 1? 2.0 ng (20 ?l) were injected into the vitreous of 8 left eyes of 8 SD rats while steriled PBS were injected into 8 right contralateral eyes of the same rats as control. All eyes were assessed by direct ophthalmoscopy every day and enucleated on the 7 th postoperative day. Histological examination (hematoxylin eosin staining) and immunohistochemical staining with antibody against VEGF antigen were performed, and sections were observed and photographed under light microscopy. Results ①All 8 IL 1? injected eyes developed epiretinal membranes and extraretinal neovascularization on the 3 rd postoperative days while none of the 8 control eyes exhibited any abnormal retinal vascular changes and they were confirmed by HE staining;②Immunostaining identified VEGF express mainly in the inner layer of vessel walls, the epiretinal membranes, the neuroganglional layer and the photoreceptor layer of retina, while the control eyes showed only weak positive staining in the photoreceptor layer. Conclusions IL 1? is capable of inducing vitreo retinal neovascularization,and increasing the expression of VEGF in the retina and epiretinal membranes.