1.Surgical jaundice after laparoscopic cholecystectomy:Analysis of 16 cases
Hongye WANG ; Lixin WANG ; Shibing SONG
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To discuss the causes,treatment and prophylaxis of surgical jaundice after laparoscopic cholecystectomy (LC). Methods A retrospective analysis was carried out in 16 cases of surgical jaundice consisting of 14 cases from this hospital out of 3 092 cases of LC from January 1994 to January 2002 and 2 cases transferred from other hospitals. Results Out of the 16 cases,residual bile duct stones were found in 9 cases,and bile duct injuries had taken place in 7 cases (2 cases of cystic duct stump leakage,3 cases of common bile/hepatic duct injuries and 2 cases of recurrent jaundice resulted from aberrant bile duct leakage).All the 16 cases were cured by surgery and no bile duct stricture occurred after surgical procedures. Conclusions Strict following indications of LC and normative surgical performance are the keys to the decrease of surgical jaundice after LC.The patient should be given active surgical intervention once surgical jaundice occurs.
2.Experimental study on Baicalein inhibiting the invasion and migration of human breast cancer cells
Xiufeng WANG ; Qianmei ZHOU ; Shibing SU
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To explore the effect of Baicalein and its mechanism on the invasion and migration of human breast cancer MDA-MB-231 cells.Methods The effect of Baicalein on cell viability was tested by MTT.The cell invasion and migration were determined by transwell chamber model.The cell movement capability was determined by cell scratch assay.The expressions of matrix metalloproteinase(MMP)2 and 9,urokinase-type plasminogen activator(uPA)were detected by Western blot.Results 50,100 ?mol?L-1 of Baicalein inhibited significantly cell invasion(P
3.Determination of PMEA-Na in dog plasma by liquid chromatography and tandem mass spectrometry and its pharmacokinetic study
Wenyan WANG ; Zilong SHEN ; Shibing WANG ; Quansheng YAO
Chinese Journal of Clinical Pharmacology and Therapeutics 2006;11(4):406-409
AIM: To established an HPLC/MS/MS method for the study of pharmcokinetics of PMEA-Na (the mono-sodium salts of 9-[2-(phosphonomethoxy) ethyl] adenine) in beagle dogs. METHODS: PMEA-Na and internal standard 9-(3-phosphony-methoxypropyl) adenine were isolated from plasma by protein precipitation with methanol, and then analyzed adopting multiple reaction monitoring (MRM) mode. Using Xterra MS column, the mobile phases consisted of methanol:water:formic acid (25:75:0.5) at a flow rate of 0.25 ml·min-1. Beagle dogs received the intravenous dosage of PMEA-Na at 1.0, 3.0 and 6.0 mg·kg-1. Pharmacokinetic parameters were obtained from concentration-time curves by non-linear least-squares regression using the program DAS. RESULTS: The linear calibration curve was obtained in the concentration range of 0.02 to 20 mg·L-1 (r=0.999), and the limit of quantitition was 20 μg·L-1. The within-day and internal-day precisions (RSD) were less than 6.5% and 10.8%, respectively. The accuracy was 97.1%~107.3%. After a single dose studies in dogs the AUC were 2.3±0.5, 8.2±1.3 and 18.5±1.3 mg·L-1·h; the t1/2 were 3.9±1.8, 8.4±1.5 and 8.9±0.6 h; the CL were 0.44±0.09, 0.35±0.05 and 0.31±0.03 ml·h-1·kg-1 at the dose level of 1.0, 3.0 and 6.0 mg·kg-1 respectively. CONCLUSION: The analytical method is sensitive and specific for investigation the pharmacokintics of PMEA-Na in beagle dogs.
4.Investigation of the infection of Rotavirus in children in Hangzhou area
Shibing YANG ; Sunyao WANG ; Yue HE ; Liming GONG
Chinese Journal of Primary Medicine and Pharmacy 2010;17(6):751-752
Objective To research the infection of Rotavirus in children in Hangzhou area and to get the message of the feature of epidemiology. Methods Stool specimens of 156 children with suspected acute viral enteritis in Hangzhou between September 2006 and January 2008 were collected. Rotavirus were detected for all specimens by enzyme linked immunosorbent assay(ELISA). Results Rotavirus was detected in 86 of 156(55. 1% ) specimens;all the 86 strains belonged to group A. The highest frequency of group A RV detected was 57% (70/123) in patients aged 6 ~ 24 months. Conclusion The major pathogen of acute viral enteritis in children in Hangzhou area between September 2006 and January 2008 was RV. Children under the age of 2 years seemed to be more susceptible to RV infection than those of other age groups. The symptoms of RV enteritis were more severe than those of other viral enteritis.
5.Analytical study of community health services quality based on 5-gap model
Dahong GAO ; Rui GUO ; Shibing YANG ; Yadong WANG ; Wannian LIANG
Chinese Journal of General Practitioners 2010;9(1):41-43
In the marketing theory, service disparity model is a simple effective tool, capable of figuring out the five clusters of reasons leading to service failure. This article applies it in the community health service domain. Through the efforts of healing the five gaps in service delivery, including Consumer expectation-management perception gap, Management perception-service quality specification, Service quality specifications-service delivery gap Consumer expectation-management perception gap and Service delivery-external communications gap, we hope to improve the community health service quality radically.
6.Classification and modeling of traditional Chinese medicine syndromes in patients with post-hepatitic cirrhosis by partial least-squares.
Lei WANG ; Yue SU ; Qin ZHANG ; Ping LIU ; Shibing SU
Journal of Integrative Medicine 2008;6(11):1122-8
To investigate the classification and modeling of traditional Chinese medicine (TCM) syndromes in post-hepatitic cirrhosis by partial least-squares (PLS) method, and to study the clinical application of PLS method in TCM research.
7.Application of Cutting-edge Techniques of Life Science in Research on Disease-Syndrome Combination
Xiaoyan LI ; Bin WEI ; Meidong ZHU ; Yunquan LUO ; Wenhai WANG ; Shibing SU
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(10):132-136
Research on disease-syndrome combination is the main approach and method of research on integrative traditional Chinese and Western medicine. Disease-syndrome combination embodies the complementary advantages of traditional Chinese medicine and Western medicine. It discusses the relationship between diseases and syndromes through an interdisciplinary approach, and explores rules of disease diagnosis and treatment based on differential diagnosis. Thanks to the development of modern life science techniques, research on disease-syndrome combination has made great achievements. This article mainly introduced the application and the development prospects of new techniques, such as data mining, system biology, epigenetics, biological network and network pharmacology in the research on disease-syndrome combination in recent years, with a purpose to provide the ideas and the methods for further research and clinical application.
8.Orthotopic liver transplantation in the elderly patients
Jiong YUAN ; Shibing SONG ; Dianrong XIU ; Jianping ZHU ; Dechen WANG ; Bin JIANG ; Wei FU ; Tonglin ZHANG
Chinese Journal of General Surgery 2001;0(08):-
Objective To assess the outcome of liver transplantation in patients over sixty years of age,in order to attempt to expand the indications of liver transplantation. Methods From August,2000 to January,2002, the clinical data of 36 patients who underwent liver transplantation in our department were analysed retrospectively. Of the 36 cases, the data (operating time, the length of hospitalization, rejection rate and ICU stay days after operation) of 5 patients whose age were ≥60 years (elderly group) and the data of 31 patients whose age were under 60 years (
9.Use of nasojejunal tube in early enteral nutrition in severe traumatic brain injury patients under mechanical ventilation
Huaxue WANG ; Qiang WU ; Shibing ZHAO ; Ximing DENG ; Shengyong ZHENG ; Xiandi HE
Chinese Journal of Clinical Nutrition 2015;23(1):23-26
Objective To evaluate the use of nasojejunal tube in early enteral nutrition in severe traumatic brain injury (STBI) patients under mechanical ventilation.Methods STBI patients requiring mechanical ventilation in intensive care unit (ICU) of the First Affiliated Hospital of Bengbu Medical College admitted in 2013 were randomly divided into the jejunal tube group (n =15) and gastric tube group (n =19).We compared the 2 groups in terms of the tolerable beginning time of enteral nutrition (EN),the time before reaching target feeding volume,the incidences of gastrointestinal complications and ventilator-associated pneumonia (VAP) during EN,mechanical ventilation time,ICU hospital stay,and 28-day mortality rate.Results The tolerable beginning time of EN [(51.73 ± 9.16) hours vs.(81.11 ± 11.82) hours,t =7.920,P <0.05] and the time required to reach target feeding volume [(87.27 ± 9.99) hours vs.(152.05 ± 28.74) hours,t =8.320,P < 0.05] in the jejunal tube group were significantly shorter than those in the gastric tube group.In the process of EN,compared with the gastric tube group,the incidences of gastric retention (6.7% vs.57.9%,x2 =10.937,P < 0.05),reflux (0% vs.36.8%,x2 =9.566,P < 0.05),vomiting (20.0%.vs.63.2%,x2 =6.642,P<0.05),aspiration (6.7% vs.42.1%,x2 =6.087,P<0.05),VAP (33.3% vs.73.7%,x2 =5.536,P < 0.05) in the jejunum tube group were significantly lower.The mechanical ventilation time [(10.73 ± 4.68) days vs.(15.74 ± 2.54) days,t =3.730,P<0.05] and the ICU hospital stay [(13.60 ± 4.80) days vs.(17.42 ± 4.05) days,t =2.497,P <0.05] of the jejunum tube group were significantly shorter than those of the gastric tube group.Comparison of 28-day mortality rate between the two groups revealed no statistically significant difference.Conclusion Early implementation of EN via nasojejunal tube in mechanically ventilated STBI patients can alleviate feeding intolerance,shorten the beginning time of EN and the time required to reach target feeding volume,reduce the incidence of complications,and shorten mechanical ventilation time and hospital stay in ICU.
10.Mesohepatectomy for treatment of central liver tumors
Changming WANG ; Shibing SONG ; Bin JIANG ; Yimu JIA ; Dianrong XIU ; Tonglin ZHANG
Chinese Journal of Hepatobiliary Surgery 2010;16(8):563-566
Objective To summarize the initial experience in application of mesohepatectomy for the treatment of central liver tumors while focusing on its indication, short-term and long-term prognosis and especially outlining its technical details. Methods The clinical data of 3 patients receiving mesohepatectomy from December 2007 to March 2009 in our hospital were retrospectively analyzed.The imaging characteristics were summarized to convey the indication details. The technique details of the operation were outlined so as to reduce blood loss or other complications during and after the operation. The post-operative course and follow-up data were also collected and analyzed. Results Two patients suffered from primary hepatocellular carcinoma with liver cirrhosis and the other from giant hemangioma. The tumors were located in Couinaud yegment Ⅳ and/or Ⅷ. The average blood loss and operative duration were 800 ml and 7 h, respectively. Blood liver function tests returned to normal within 7 days postoperatively and the patients were discharged after that. No complications occurred. The follow-up for 7-15 months showed that there was no recurrence. Conclusion Mesohepatectomy is the principal choice of treatment for centrally located liver tumors. For the safety and avoidance of complications, the doctor should abide by the concept of segment-oriented hepatectomy and apply the updated techniques such as CUSA (Cavitron ultrasonic surgical aspirator). For patients with compromised liver function, mesohepatectomy might be superior to extended bepatectomy. Thus, the application of mesohepatectomy should be expanded. Meanwhile, further investigation is needed for its full evaluation.