The evaluation of biomaterial biocompatibility is a key step before the clinical application.With the rapid development of molecular biology,scientists have begun to evaluate biomaterial biocompatibility at molecular level and proposed the concept of molecular biocompatibility.Researchers'main tasks at present are to identify more molecular markers using molecular biology technology and to establish standards for evaluating molecular biocompatibility of biomaterial,which will provide the guidance for the design of better biomaterials.

By using electron microscopy histochemical method, light microscopy histochemical method and microspectrophoto meter, we studied the ACP activity of the principal cell of epididymal duct in the postnatal 10-90 days rats. The results revealed that ACP activity increased with the increase days. We also observed the following phenomena: in the postnatal 10 days rats the ACP activity was only localized on GERL and vesicles around GERL; by in the postnatal 19 days rats, the ACP activity was localized on lysosome; and 35 days later, the ACP was also localized on the saccules on the forming face of Golgi complex. The significance of the above changes in ACP activity of the principal cell of epididymal duct in postnatal rats was discussed in this paper.

Activity and localization of y-glutamyl transpeptidase (?-GT) , ?-Naph-thyl acetate esterase (ANAE) , alkaline phospbatase (AKP) and glucose-6-phosphatase (G-6-P) in the epididymis of the rat has been investigated histo-chemically by light microscopy.?-GT activity was mainly localized on the supranuclear region and the ste-reocilia of the epithelial cells. The intensity in the epithelium of the segment Ⅱ is the highest of the rat epididymis. There was a gradual decrease in the intensity of this enzyme from segment Ⅲ through Ⅳ and it was absent from the segment Ⅳ. Activity of ANAE in the segment Ⅳ, Ⅴ and Ⅵ was higher, but that in the segment Ⅰ,Ⅱ and Ⅳ was less. ANAE activity was localized throughout the cytoplasm of the epithelial cells in the segment Ⅰ,Ⅲ, Ⅴ and Ⅵ. Whereas the activity in the segment Ⅱ and Ⅳ was localized on the basal region of the epithelial cells. AKP activity was present in the basement membrane of the epididymal epithelium and the bloodvessel wall of subepithelial connective, tissue of the epididymal duct. G-6-P was distributed homogenously in the cytoplasm of the epithelial cells throughout the epididymal duct.The possible function of these enzymes in the rat epididymis are briefly discussed.

By the routine electron microscopy the ultrastructure of the epididymis of 10 rats is studied.Except the principal cells,there is no significant difference in the ultrastructure of the other cell types of the epididymal epithelium between segments.The principal cell shows the ultrastructural characteristics of secretion and absorption.However,the ultrastructural characteristics of the principal cells in the proximal epididymvs show more tendency to absorbent and that in the distal epididymis show more tendency to secretory.Further-more it is observed that some halo cells are surrounded by small cells which possess long processes.Some or a few number of clear cells in the caudal epididymis contain some crystaloid inclusions in their basal cytoplasm.The presence of the fenestrated capillary in the peritubular tissue is also found.The meaning of the ultrastructures mentioned above is discussed.

Guinea pig, pigeon and toad are the vertebrates on different evolution level. The acid phosphatase content in the hepatocytes of centrilobular area and periportal area of hepatic lobules were quantitatively measured by microspectrophotometer and image analyser. The results showed that the content of acid phosphatase of hepatocytes was highest in guinea pig, lowest in pigeon, and toad in between the both. The average density of acid phosphatase distribution in the hepatocytes increases according to the evolution order. The significance of this phenomenon was discussed.

Objective To explore differences of ultrasoundgraphy and CT guided percutaneous paracentesis for liver abscess and provide references for the paracentesis treating of liver abscess .Methods 68 patients with liver abscess arranged ultrasoundgraphy guided percutaneous paracentesis were regarded as ultrasound group ,and 38 patients accepted CT guided percutaneous paracentesis were considered as CT group .The succeed rate of paracentesis ,operation period ,treatment effectiveness ,complications of the 2 groups were compared .Results The succeed rate of paracentesis in ultrasound group (81 .7% ) was not different obviously to CT group(82 .6% ) (χ2 =0 .016 2 ,P=0 .898 6) .The operation period of ultrasound group(22 .3 ± 5 .6)min was shorten than CT group (25 .6 ± 4 .8)min ,t= 3 .057 4 ,P< 0 .05 .The heal rate of the 2 groups(91 .2% ,92 .1% ) was not different (χ2 = 0 .027 1 ,P=0 .869 3) .There was no different of the complications in the 2 groups(5 .88% ,8 .57% )(χ2 =0 .010 1 ,P=0 .920 1) .Conclusion Either ultrasoundgraphy or CT guided percutaneous paracentes is feasible and safe for liver abscess .Compared to CT ,the ultra-soundgraphy is more convenient and economic .

Objective To evaluate the clinical curative effect of Salvia miltiorrhiza Ligustrazine combined with Butylphthalide in cerebral infarction of children. Methods Sixty-two children with acute cerebral infarction were randomly divided into treatment group (33 patients) and control group (29 patients). The patients in two groups received the same basic treatment. The control group used Salvia Ligustrazine injection, and the treatment group used Salvia Ligustrazine injection combined with Butylphthalide. The total efficiency, the degree of recovery of muscle strength and the scores of nerve defect 2 weeks after treatment were compared. Results The total efficiency in treatment group was 87.9%(29/33), in control group was 75.9%(22/29), and there was significant difference (P<0.05). Two weeks after treatment, the muscle strength scores in treatment group was (4.00 ± 0.47) scores , in control group was (3.59 ± 0.98) scores, and there was significant difference (P<0.05). Two weeks after treatment, the scores of nerve defect in treatment group was (5.42 ± 2.18) scores, in control group was (6.86 ± 2.23) scores, and there was significant difference (P<0.05). Conclusions Salvia Ligustrazine combined with Butylphthalide in treatment of cerebral infarction of children has obvious effect, and it is worthy of spreading.

Temporomandibular joint (TMJ) diseases are common clinical conditions. The number of patients with TMJ diseases is large, and the etiology, epidemiology, disease spectrum, and treatment of the disease remain controversial and unknown. To understand and master the current situation of the occurrence, development and prevention of TMJ diseases, as well as to identify the patterns in etiology, incidence, drug sensitivity, and prognosis is crucial for alleviating patients′suffering.This will facilitate in-depth medical research, effective disease prevention measures, and the formulation of corresponding health policies. Cohort construction and research has an irreplaceable role in precise disease prevention and significant improvement in diagnosis and treatment levels. Large-scale cohort studies are needed to explore the relationship between potential risk factors and outcomes of TMJ diseases, and to observe disease prognoses through long-term follw-ups. The consensus aims to establish a standard conceptual frame work for a cohort study on patients with TMJ disease while providing ideas for cohort data standards to this condition. TMJ disease cohort data consists of both common data standards applicable to all specific disease cohorts as well as disease-specific data standards. Common data were available for each specific disease cohort. By integrating different cohort research resources, standard problems or study variables can be unified. Long-term follow-up can be performed using consistent definitions and criteria across different projects for better core data collection. It is hoped that this consensus will be facilitate the development cohort studies of TMJ diseases.