Objective To design a stimulating instrument for physiology experiment.Methods A sine wave was produced by a high frequency oscillator,and then the sine wave increased in voltage by a transformer to accord with the maximal range needed.Afterwards,the increscent sine wave became a direct current signal with high voltage by a rectifier and a filter.Finally,the signal became a stimulating impulse to be exported by a switch,which was controlled by a controlling signal with adjustable frequency and width.Results Voltage range adjusted between 1 and 80V;Frequency adjusted between 0.1 and 200Hz;Impulse width adjusted between 0.1 and 5ms.Conclusion The stimulating instrument designed can be used to do electricity stimulating experiment in Physiology.

To investigate the effect of premedication with clonidine on the concentrations of plasma catecholamine (CA), renin, angiotension Ⅱ (A Ⅱ ) and carbohydrate metabolism during eardiopulmonary bypass (CPB). Method: Twenty patients scheduled for cardiac surgery were randomly divided into two groups: clonidine group and control group. Oral premedication with clonidine 5?g?kg~(-1) was taken in colndine group 60 rain before anesthesia in duction in addition to common same premedication in both groups. Arterial plasma concentrations of CA,renin, AⅡ, blood suger, pyruvic acid, lactic acid were measured before anesthesia, before CPB, 30,60,90 and 120 min following CPB and 30 rain after CPB. Result: The levels of CA, blood suger, pyruvic acid and lactic acid increased significantly during CPB in both groups, but were higher markedly in control group than those in clonidine group (P

Objective To describe the importance of anesthesia management in performing interventional procedures. Methods A total of 24 382 patients, who were admitted to authors’ hospital during the period from April 2011 to April 2015 to receive level Ⅲ or level Ⅳ interventional procedures, were enrolled in this study. According to the anesthesia method, the patients were divided into (1) mechanical ventilation group (group A), i.e. intravenous general anesthesia combined with laryngeal mask or endotracheal intubation, (2) intravenous general anesthesia and autonomous respiratory group (group B), (3) conscious sedation group (group C) and (4) local anesthesia group (group D). The heart rate (HR), mean arterial pressure (ABP), blood oxygen saturation (SpO2) and anesthesia-related complications of the patients of all four groups were kept under close observation before, during and after the interventional procedures, the results were statistically analyzed. Results The anesthesia was successfully implemented according to the operation plan in all 24 382 patients. Interventional procedure of level Ⅲ was performed in 16 702 patients(68.5%) and interventional procedure of levelⅣwas adopted in 7 680 patients (31.5%). The patients receiving interventional procedure of level Ⅲof group A, B, C and D were 6 797 (40.7%), 3 608 (21.6%), 5 095(30.5%) and 1 202(7.2%) respectively;while the patients receiving interventional procedure of level Ⅳ of group A, B, C and D were 4 193 (54.6%), 2 527 (32.9%), 699 (9.1%) and 261 (3.4%)respectively. No statistically significant differences in preoperative HR, ABP and SpO2 existed between each other among the four groups (P>0.05). In group A, B and C the HR and ABP values determined in operation were not statistically different from the preoperative ones(P>0.05), and the differences in HR and ABP values among the three groups were also not statistically different (P>0.05);SpO2 levels showed no obvious changes (P>0.05). In group D, the HR and ABP values determined in operation were significantly higher than the preoperative ones (P<0.05), and also these values were statistically different from those of other three groups (P<0.05);SpO2 levels showed no obvious changes (P>0.05). In 22 patients of group D the operation had to be stopped as they were unable to tolerate the procedure. Conclusion In performing different levels of interventional procedures, level Ⅲ and level Ⅳ intervention surgeries in particular, careful selection of individualized anesthesia plan on the base of patient’s condition and operation requirement is an important guarantee for ensuring a safe operation with no interference, and it is also a good way to reduce the pain severity of patient. Therefore, individualized anesthesia plan is worth to be widely used in interventional procedures.

Aged ; Aging ; Health Services for the Aged ; Humans

Objective To explore the differences between the conservative treatment and the operative treatment in the management of cervical spinal cord injury, and the timing of the operation. Methods The therapeutic effectiveness o f the 45 cases who were treated from October 1996 to January 2002 was analyzed. 21 of them underwent conservative treatment and 24 operative treatment. The corr elation between the therapeutic effects of the operation and its timing was expl ored. Results The myeloid functional recovery was poor in the patients who had b een treated with conservative means but good in those who had been given an oper ation. The earlier the operation, the more effective it could be, and the higher the probability of the myeloid functional recovery. Conclusions The operative treatment promises better outcome than the conservative treatment. To get the be st effects, the operation should be done within one month after the injury, for the longer the lapse between the injury and the operation, the worse the effect.

Objective To study the effects of podophyllotoxin solid lipid nanoparticles (POD-SLN) on the proliferation of human epidermal cells in vitro. Methods POD-SLN was prepared by using microemulsion technique, the morphology of POD-SLN was examined by transmission electron microscope (TEM), and its particle size and Zeta potential were studied by Zetasizer analyzer. High-performance liquid chromatography (HPLC) was employed to determine the entrapment efficiency of podophyllotoxin (POD) in the nanoparticles, and its stability was observed. Human epidermal cells were treated with different concentrations (0.1-1 000 ?g/L) of POD-SLN, and the proliferation of human epidermal cells was studied at different time points after exposure (6h, 12h, 24h, 48h). The cytotoxic effects of POD-SLN, POD liposome, free POD, blank solid lipid nanoparticles (SLN) and control groups on human epidermal cells were assessed using a colorimetric MTT cell viability assay. Results POD-SLN displayed spherical or elliptical in shape, and it was stable. The average particle size of POD-SLN was 87.2?10.3nm, Zeta potential was 25.3?0.8mv and the entrapment efficiency of POD in the nanoparticles was 83.2%?2.5%. POD-SLN inhibited the proliferation of human epidermal cells in a concentration- and time-dependent manner. At the same concentration, the effect of PDP-SLN on anti-proliferation was stronger than that of POD liposome and POD. The inhibition of human epidermal cells after 48h exposure to PDP-SLN, POD liposome, and POD reached 91.05%, 77.02% and 68.46% respectively, at the highest concentration of 1000?g/L, and the IC50 were 2.11?g/L, 16.65?g/L and 101.42?g/L, respectively. Blank SLN had no effect on the proliferation of human epidermal cells. Conclusion This formulation and technology are stable and practical. POD-SLN can significantly inhibit the proliferation of human epidermal cells in vitro and the inhibitory effect was better than that of POD liposome and POD.

0.05) after SVT episode. Compared to the SVT group and basic status, the extraction ration of glucose and oxygen of CAD patients obviously increased( P

AVNRT, (3)There was no evidence of new arrhythmia occurring for the late electrophysiologic sequelae of RFCA lesions. Conclusion The technique of RFCA is safe, it causes significant minor myocardial injury and takes 3 months to develop from initially resembling coagulation necrosis to fibrosis. There is no evidence of new arrhythmia occurring for late electrophysiologic sequelae of RFC lesions.

Objective : To evaluate the efficacy and safety of Morita therapy combined with citalopram in the treatment of obsessive-compulsive disorder(OCD). Methods: Fifty-six patients with OCD were randomized to two groups. The patients (N=28 ) in observed group were treated with Morita therapy combined with citalopram. The patients (N=28 ) in control group were treated with citalopram alone. The efficacy and safety were assessed with Y-BOCS, CGI-SI , HAMD and TESS scales. Results : The scores of Y-BOCS , CGI-SI and HAMD scales in observed group were significantly lower than that in control group at the end of 4 weeks, 8 weeks and 6 month after the treatment (P0.05) . Conclusion: Morita therapy combined with citalopram was more effective than citalopram mono-therapy in the treatment of OCD.