AIM:To investigate the effect of Gln deprivation on growth and differentiation of primary APL cells. METHODS: The primary APL cells were collected from 18 cases of APL patients' periphery blood, the patients had not been treated. The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10% fetal calf sera, at 37℃,5% CO 2 and saturation humidity for 4 days.The initial living cell density was 5?10 8/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens. RESULTS: The living cell density became (54.28?4.28)% of the initial in glutamine deprivation group, but the living cell density in control with 4 mmol/L Gln was (108.56?12.27 )% of the initial ( P

Animals ; Anti-Inflammatory Agents ; pharmacology ; Cyclooxygenase 2 ; metabolism ; Inflammation ; metabolism ; Insulin ; pharmacology ; Nitric Oxide Synthase Type II ; metabolism ; RNA, Messenger ; genetics ; Rats

Cardiac Catheterization ; methods ; Heart Defects, Congenital ; therapy ; Humans

Arginine ; therapeutic use ; Burns ; therapy ; Humans ; Nutrition Therapy ; Wound Healing

Burns ; Periodicals as Topic ; Surgery, Plastic

T helper 17 cells (Th17) are a novel subset of CD4+ T cells that undergo differentiation from naive CD4 T cells through the activation of a key nuclear transcription factor,retinoic-acid-receptor-related orphan receptor γt (RORγt),and in the presence of multiple cytokines,such as transforming growth factorβ1 (TGF-β1),interleukin6 (IL-6),as well as IL-23.Th17 cells induce their biological effect by secreting IL-17A and other cytokines.Research has demonstrated that Th17 cells have an intimate relationship with acute/chronic transplant reactions and that Th17 cell lineages have the possibility to become the new target for treating transplant rejections.Identification of Th17 cells also provides an additional strategy for inducing immune tolerance.

Chemical constituents in the mature fruit peels of Sinocitrus chuana Hort were studied systematically. Five compounds were isolated and identified. They were hesperidin( Ⅰ), nobiletin(Ⅱ) tengeretin(Ⅲ), 4', 5, 7, 8-tetramethoxyflovones(Ⅳ) and synephrine(Ⅴ). Of them the fourth was isolated from this genus for the first time.

Enteral Nutrition ; methods ; Humans ; Intestines ; pathology ; physiopathology

Animals ; Burns ; drug therapy ; metabolism ; Glutamine ; pharmacology ; therapeutic use ; Humans ; Wound Healing ; drug effects

Objective To study the clinical and THR?gene mutation of two cases of pituitary thyroid hormone resistance. Methods The clinical data and peripheral blood samples of the patients and their family members were collected, and then DNA was isolated. PCR and direct sequencing techniques were performed to determine if there were mutations in their THR?gene. Results No mutation was found in exon 1-10. Conclusion Pathogenesis of sporadic pituitary resistance to thyroid hormone usually has no relation with mutation of THR?gene.