Objective To explore the value of fractional exhaled nitric oxide (FeNO) in diagnosis and treatment of asthma-COPD overlap syndrome (ACOS).Methods A total of 195 patients categorized into elderly ACOS,asthma,COPD and chronic cough groups were consecutively admitted from September 2013 to June 2014 in our hospital.All patients were examined with chest imaging,fractional exhaled nitric oxide (FeNO) test,and pulmonary function tests to analyze the differences in FeNO,FEV1/pred％ and FEV1/FVC among four groups,and to detect the relevance of FeNO to FEV1/pred％ and FEV1/FVC within each group.Results Both of the ACOS and asthma groups had higher FeNO values than the COPD and control groups [(30.9 ± 27.8,34.5 ± 29.4) vs.(17.3 ± 9.9,16.1 ± 8.6),P ＜ 0.05],while the values of FEV1/pred％ and FEV1/FVC in the ACOS and COPD groups were significantly lower than those in the other twogroups [FEV1/pred％,(52.9±8.4,53.5 ±9.6) vs.(81.1 ±5.9,85.7 ±7.1),allP＜0.05; FEV1/FVC,(51.9 ± 7.2,50.7 ± 7.1) vs.(79.2 ± 4.8,81.0 ± 5.9),all P ＜ 0.05].Although FeNO was directly related to FEV1/pred％ in the control group,there were no significant correlation between FeNO and FEV1/pred％ (or FEV1/FVC) in the other three groups [ACOS group,r =0.115,-0.007 (P =0.464,0.963) ; asthma group,r =0.038,0.045 (P =0.772,0.733) ; COPD group,r =0.097,0.010(P=0.498,0.944); control group,r =0.315,0.091 (P =0.045,0.571)].Conclusions The combination of FeNO and pulmonary function tests can facilitate the differential diagnosis of ACOS and other chronic obstructive airways disorders.For the elderly COPD patients with higher FeNO values,it should be noted whether they were complicated by ACOS or not.Furthermore,the inhalation of corticosteroid can be used to reduce the airway inflammation in these patients.

Objective To investigate the effects of long non-coding RNA(LncRNA) growth arrest specific transcript 5(GAS5) negatively regulating nucleophosmin 1(NPM1) on cisplatin(DDP) resistance of gastric cancer cells.Methods The normal human gastric mucosa cell line GES-1 and human gastric cancer cell lines BG3-823,MGC-803 and AGS were selected as the research objects,of which the level of LncRNA GAS5 in each cell was measured by qRT-PCR.The drug resistance of AGS cells to DDP(AGS/DDP) was induced,and the experiment was divided into control group,empty plasmid group(BC group),GAS5 nonsense interference group(pLJM-GAS5 NC group) and GAS5 overexpression group(pLJM-GAS5 group).MTT method was used to determine the effect of DDP on the proliferation of AGS and AGS/DDP cells;and the levels of NPM1,multidrug resistance 1(MDR1),excision repair cross complementation group 1(ERCC1),multidrug resistance-associated protein 1(MRP1) and N-cadherin in AGS and AGS/DDP cells were measured by Western blot.Results Compared with the normal gastric mucosa GES-1 cells,the level of LncRNA GAS5 in BG3-823 and AGS cells decreased significantly,and among them,the level of LncRNA GAS5 in AGS cells was the lowest,so AGS cells were used for the follow-up experiments.Compared with the control group,the level of LncRNA GAS5 in AGS cells of BC group and pLJM-GAS5 NC group decreased significantly,while the levels of NPM1,MDRl,ERCC1,MRP1 and N-cadherin increased significantly;compared with BC group and pLJM-GAS5 NC group,the level of LncRNA GAS5 in AGS/DDP cells of pLJM-GAS5 group increased significantly,while the levels of NPM1,MDR1,ERCC1,MRP1 and N-cadherin decreased significantly;after treatment with DDP of the same concentration(except 0 μmol/L),compared with the control group,the inhibition rate of AGS/DDP cell proliferation in BC group and pLJM-GAS5 NC group decreased significantly;compared with BC group and pLJM-GAS5 NC group,the inhibition rate of AGS/DDP cell proliferation in pLJM-GAS5group was significantly higher.The semi inhibitory concentration(IC_(50)) of DDP on AGS/DDP cells in pLJM-GAS5 group for 48 h was(65.38±5.04) μmol/L,which was significantly lower than(120.74±4.17) μmol/L and(120.24±4.29) μmol/L in BC group and pLJM-GAS5 NC group.Conclusion Up-regulating the level of LncRNA GAS5 in AGS/DDP cells can reverse the drug resistance of AGS/DDP cells,which may be related to the down-regulation of NPM1expression

Objective To systematic evaluate the prevalence of secondhand smoke among Chinese mainland rural residents by meta?analysis,so as to provide reference for making relevant smoking control regulations and the carry out of targeted tobacco control action. Methods The related stud?ies were searched and collected from PubMed,CNKI,Wanfangdata,VIP and CBM database,which were published between 2001 and 2015 about the prevalence of secondhand smoke among Chinese mainland rural residents. Meta?analysis was tested by software CMA V2. Results The pooled prevalence of secondhand smoke among Chinese mainland rural residents was 46.6%(95%CI 40.5%?52.8%). Stratified analysis showed that the prevalence of secondhand smoke were 45.7%and 47.5%for male and female,45.7%and 51.1%for eastern and midwest China,22.1%、34.2%and 71.3%for public place,workplace and family,respectively. The prevalence among sex,regions,and places were statistically significant. Conclusion The prevalence of secondhand smoke among Chinese mainland rural residents was relatively high,and the prevalence of secondhand smoke was higher in women than in men,in midwest area than in eastern area,and at family than at public place and at workplace.

BACKGROUND; Studying the pathogenesis and rule of intestinal barrier damage under exercise stress will provide theoretical evidence for preparing intestinal barrier protectant under this state.OBJECTIVE : To observe the changes in intestinal barrier of rats following different intensities of swimming exercise. DESIGN: Randomized controlled animal experiment.SETTING: Laboratory of Exercise Sciences & Sports Medicine, Huibei University; Basic Laboratory, Medical College, Wuhan University.MATERIALS: Thirty-six healthy male SD rats, aged 3 weeks, were involved and randomized into 3 groups: control group (n =10), proper exercise group (n =12) and excessive exercise group (n =14). The rats of three groups were raised in the same condition.METHODS:①Control group: Rats did not exercise normally. ②Proper exercise group: Rats swam without loading. In the first 3 days, they adaptatively swam for 30 minutes and gradually for 60 minutes within 1 week, then they swam once a day, 6 times a week, 6 weeks in total. ③Excessive exercise group: In the first 3 days, they adaptatively swam for 30 minutes and gradually for 120 minutes within 1 week. After trained for 1 week, they were given excessive swimming training. Then, they were forced to swan once a day, 6 times a week, within 4 weeks successively. Within later 2 weeks, the rats were forced to swan once in the morning and evening separately, 6 times a week.MAIN OUTCOME MEASURES:①Intestinal barrier parameter: intestinal mucosal permeability, plasma endotoxin, becterium shift rate.②Intestinal mucosal membrane structure.RESULTS: Thirty-six rats were involved in final analysis.①After excessive exercise, plasma endotoxin of rats was doubly increased, intestinal mucosal permeability was enhanced by 2.5 times, and bacterium shift rate was increased by 230%.②Proper exercise had no obvious influences on the structure of intestinal mucosal membrane tissue of rats, and excessive exercise expanded Golgi complex in the intestinal epithelial cells and rough endoplasmic reticulum of rats, caused severe edema of epithelial cells and infiltration of inflammatory cells.CONCLUSION: Proper exercise improves intestinal function of body; excessive exercise causes intestinal barrier injury of body and pathological syndrome of digestive system.

Antiviral Agents ; therapeutic use ; Hepatitis C ; drug therapy ; Humans ; Liver Cirrhosis ; drug therapy ; virology

One of the key contents in tissue engineering trachea replacement research is the scaffold selection.This review summarizes the latest original literatures and investigations about electrospun technique as well as recent progress.To discuss the advantages and disadvantages of natural,synthetic and combined electrospun scaffolds,the versatility in material choosing and production methods is the unique superiority.For specific experimental or clinical objects,the further research is to choose a suitable polymer,to improve surface modification techniques and to control the dimension and arrangement of the fibrous structure of electrospun fibers,which can offer versatility and tissue specificity,and therefore provide a bright prospect for tissue engineering trachea.

Adrenomedullin is a vasodilative peptide found in 1993. It has been proved that proadrenomedullin can be divided into 4 peptides by endoenzyme. Proadrenomedullin N terminal 20 peptide (PAMP) is one of them. PAMP has many functions on cardiovascular physiology. But so far the relationship between PAMP and cardiovascular diseases has not been found. In this study its plasma levels in the patients with essential hypertension(EH) and heart failure(HF) were investigated with its pathophysiological effect discussed. The plasma levels of PAMP, endothilin(ET), Angiotension II(AT II) were measured with RIA, and norepinephrine(NE), epinephrine(E) were measured with HPLC. the plasma level of PAMP in the control group was 18 42?2 33ng/L, and was 1 62 times higher in EH group than in the control group, and was 1 53 times higher in HF group as compared with the control group respectively ( P

Objective To evaluate the relationship between amniotic fluid (AF) epidermalgrowth factor (EGF) concentration and fetal pulmonary maturation. Methods Lecithin － sphin-gomyelin ratio (L/S) and EGF were measured in amniotic fluid from women(n = 138,21～42 weeks'gestation) undergoing amniocentesis by TLC and RIA. ResultsThe AF L/S ratio and EGF concen-tration increased linearly with Gestational age(GA) ( r = 0.8t3 and 0.87, respectively, P < 0.01 ).There was a strong correlation between the AF L/S ratio and EGF( r = 0.76, P < 0. 001 ). Conclu-sionThere are a confirmed relationship between AF EGF levels and fetal pulmonary maturation.

Objective To explore the association of the inherent cellular level of reactive oxygen species (ROS) with the sensitivity of the gastric and esophageal carcinoma cells to arsenic trioxide (As 2O 3). Methods The difference of sensitivity to apoptosis induction by low concentration (2 ?mol/L) of As 2O 3 between the gastric carcinoma cell line SGC7901 and MKN45, and between the esophageal carcinoma cell line EC/CUHK1 and EC1867 was firstly demonstrated. SGC7901 was more sensitive than MKN45, and EC/CUHK1 more sensitive than EC1867 to As 2O 3. Then dihydrogenrhodamine 123 (DHR123), as a ROS capture, was incubated with the cells in the absence of As 2O 3. The fluorescent intensity of rhodamine 123, the product of cellular oxidation of DHR 123, was assayed by flow cytometry. The ROS levels was thus detected. Results Inherent cellular ROS level is higher in SGC7901 and EC/CUHK1. They are more sensitive to As 2O 3, than the corresponding cell line MKN45 and EC1867. Conclusions The difference in inherent cellular ROS level of gastric and esophageal carcinoma cells is associated with the cellular sensitivity to apoptosis induced by As2O 3.

Objective:To investigate the stability of Zhiling Liumiao oral liquids. Methods: The active ingredients in Zhiling Li-umiao oral liquids were determined by HPLC, and the identification was studied by TLC. Meanwhile, the other indices including char-acter, relative density, pH and microbial limit were determined as well. Results:The active ingredients in Zhiling Liumiao oral liquids could be influenced by strong light, while the changes were within the range of the quality standards, and the samples were stable in the accelerated test and long-term test. Conclusion:The designs of preparation process and package are rational to keep the stability of the preparation. The shelf time may be set at 12 months.