This review summarizes the present situation of research, development and application of the Chinese herbal feed additives and its prospect. It can provide scientific references for researchers to carry out in-depth study. Chinese herbal feed additives will hopefully solve problems such as antibiotic residues, environmental pollu-tion, low productivity which hinders the development of livestock and provides strong support for developing green husbandry, meeting the food safety demand, closing the gap of livestock between the developed countries and Chi-na, increasing competitiveness of Chinese animal products in world markets. The development of Chinese herbal feed additives have very important economic and social benefits.

Objective To explore the significance of calcification in diagnosis of thyroid mass , and to improve the differential diagnosis of benign and malignant thyroid nodules .Methods 286 cases with thyroid masses admitted to the hospital from Jan .2008 to Dec.2012 underwent preoperative ultrasound scan .The clinical data were retrospectively analyzed from patients with or without calcification , the characteristics of different calci-fication and the comparison between preoperative diagnosis and postoperative pathological results .Results Ultra-sound and pathological analysis showed there was significant difference between calcification group and non -calci-fication group in detection rate of thyroid cancer (66.98%vs 13.51%, P<0.05).Detection rate of thyroid car-cinoma was significantly higher in microcalcification group than in coarse calcification group ( 84.42% vs 20.69%,P<0.05) .There was significant difference in detection rate of benign and malignant nodules in the two types of calcification group(P<0.05).The detection rate was higher in the malignant nodules than in the benign nodules .The detection rate of thyroid cancer had no statistical significance in non-calcification group and coarse calcification group ( P>0.05 ) .Conclusion Thyroid calcification , especially microcalcification has important significance in diagnosis of thyroid cancer .

Objective To explore the diagnostic value of multiplanar reconstruction(MPR)of spiral CT in the disorders of lumbar intervertebral discs.Methods Fourty-one cases with the disorders of lumbar intervertebral discs were examined with conventional CT scans and MPR of spiral CT.CT findings of intervertebral discs at L 3/4 ,L 4/5 and L 5/S 1 were compared and observed.Results The conventional scans showed bulging and /or herniation of intervertebral discs in 35 cases,and normal in 6 cases which compression of nerveroots by intervertebral discs or osseous narrow of intervertebral discs were showed on MPR.The MPR showed compression of nerve roots,compression of dural sac or osseous narrow of intervertebral foramens in 36 cases.Conclusion MPR possesses important value in diagnosing the disorders of lumbar intervertebral discs,showing the causes of lumbar vertebral diseases and distinguishing the various diseases of lumbar vertebrae.

Objective To investigate the resistance rate of Helicobacter pylori (Hp) to clarithromycin and its correlation with point mutations in 23S rRNA gene. Methods Hp was cultured from gastric biopsy specimen obtained from 189 patients undergoing upper gastrointestinal endoscopy. The DNA of 11 clarithromycin sensitive Hp and 19 clarithromycin resistant Hp was extracted, and 23S rRNA was amplified and sequenced. Results The rate of clarithromycin resistance in cultured Hp was 29. 2%. Point mutations in 23S rRNA gene were found in 17 clarithromycin resistant Hp strains. The proportion of A to G mutation was 36.8%, G to A of 21.5%, C to T of 15.8%, A to C of 10.5% and T to C of 5.3%. No point mutation in 23S rRNA was detected in other 2 clarithromycin resistant and 11 sensitive Hp strains. Conclusion The resistance to clarithromycin is common in Hp, and point mutations in 23S rRNA gene of Hp are frequent in clarithromycin resistant strains, with most prevalent mutations of A to G and next G to A.

Hepatocyte is the core raw materials of bioartificial liver support system, primary hepatocyte is lim-ited to application because of short survival and difficult cul-ture in vitro. Porcine hepatocyte which has been used re-cently exist the risks of endogenous retrovirus transmission.With the development of molecular biology, it has been pos-sible that hepatocyte is immortalized recently. Immortalized hepatocytes have greatly significant to drug toxicology, bio-artificial liver support system and tissue engineering of liver.Therefore, we will review the prospects for research and ap-plication of immortalized hepatocytes.

Angelica ; chemistry ; Animals ; Animals, Newborn ; Cell Count ; Female ; Fetal Hypoxia ; pathology ; Hippocampus ; cytology ; drug effects ; Male ; Neuroglia ; cytology ; Neurons ; cytology ; Pregnancy ; Rats ; Rats, Sprague-Dawley

Accidents, Occupational ; Adult ; Female ; Humans ; Male ; Nitrogen Mustard Compounds ; poisoning ; Occupational Exposure ; adverse effects

Cells and scaffolds are two key elements of tissue engineering,while the viabilitv of cells is mainly depended on the nature of scaffolds.A suitable scaffold should simulate the internal environment as well as possible,which is necessary for the growth of cells.The nanometer structured biomaterials have become more and more popular in the study of tissue engineering due to their ability to perfectly simulate the topography of the microenvironment.This paper summarized the advances in the application of nanometer structured materials in the in vitro cell cultures.The mechanisms of the promoting effect of nano.structured biomaterials on the cell growth and the application of nano-structured materials in liver tissue engineering are discussed.

Objective To evaluate the value of MR-guided neurolytic celiac plexus block(NCPB)for treatment of upper abdominal cancer pain.Methods 13 neurolytic celiac plexus blocks were carried out in 12 patients with severe upper abdominal pain caused by malignant tumors.The pain-relieving effect of the block was both evaluated using visual analogue scale(VAS) and analyzed statistically in all cases.Results The placement of the needle MR-guided was easy and accurate,the successful rate of the puncture was 92%.There were no severe complications.The pain before and after the procedure had obvious difference and the pain relief could last for a long time.Conclusion MR-guided NCPB is a simple and effective technique for treatment of upper abdominal cancer pain.

[Objective] To investigate the changes of urinary deoxypyridinoline(DPD)in patients with spinal tuberculosis(STB)and evaluate their clinical significance.[Method] All subjects were divided into three groups:group 1,STB patients;group 2,Pulmonary TB patients:group 3,healthy controls.Their DPD was detected by ACS:180 automated chemiluminescence system.The values for urinary DPD were adjusted for urinary creatinine to overcome confounding variables such as body mass index and urine dilution.The results were analyzed.[Result]The mean urinary concentrations of DPD in spinal tuberculosis,pulmonary tuberculosis and healthy controls were 1(4.9?9.8)?mol/mol Cr.,(6.4?2.6)?mol/mol Cr,and(6.3? 2.0)?mol/mol Cr.,respectively(P value=0.001,0.000).Patients with spinal tuberculosis had a higher urinary excretion of DPD than those without spinal tuberculosis.There was no difference in groups of pulmonary tuberculosis and healthy controls(P=0.751).DPD had a better receiver operating characteristic(ROC)curve and the area under the ROC curve was 0.83.For DPD,the sensitivity(87%)and specificity(73%)were seen at the cut-off level of 8.4 ?mol/mol creatinine.[Conclusion]Change of bone matabolic activities can be found in patients with STB.Bone resorption can increase sharply by DPD.The determination of urinary DPD may provide an important reference value for the assessment of STB from Pulmonary TB.