Stromal cell-derived factor-1(SDF-1)is an early cell growth factor which belongs to a subfamily of chemokines.The CXCR4 is SDF-1 receptor.The SDF-1 interacting with CXCR4 plays important roles in physiological and pathological processes such as hematopiesis, stem cell homing,angiogenesis and leukemic cells infiltrating.To study the expression of CXCR4 in leukemia and the relationship between CXCR4 and leukemic cells infiltration can add indicators for leukemic MIC diagnosis of immunology indexes and have great significance to take molecule targeted therapy for preventing leukemia from infiltration or relapse as well.

Adult ; Anaphylaxis ; etiology ; Female ; Humans ; Male ; Semen ; immunology

Patent ductus arteriosus (PDA)is a common disease among premature neonates and the management of which has always been a challenge to the neonatologists. Early treatment has been advocated as the first-line approach, and drug induced closure is yet the most effective, inexpensive, and convenient method. During the last two decades,indomethacin was often given as conservative treatment, and its closure rate in PDA was approximately 46% ～ 89%. However,indomethacin may increase the number of undesirable or harmful effects including renal damage, intraventricular hemorrhage, necrotizing enterocolitis, and enterobrosis,etc. Recently,ibuprofen is rapidly emerging as a new promising drug for treatment of PDA in preterm infants with closure rate of 73.0% ～95.5%. It is used as standard therapy to close PDA,and it has less influence on the hemodynamics of kidneys, brain and gastrointestinal tract. The surgery is needed in patients with drug treatment failure and cardio-pulmonary function damage.

Based on multiple painless needle insertion skills in clinic combined with own experience, the authors propose the opinion that different painless needle insertion methods need to be used according to the positions where acupoints are. The appropriate needle insertion mean must be selected in accord with the anatomical characteristic of acupoint position, namely, tapping insertion is applied in scalp part, skin-pinching up needle insertion is used in forehead and superficial nerve trunk, fingernail-pressure needle insertion in ocular region and positions where large blood vessels distribute, flying acupuncture insertion in face and finger tip, vibrating needle in occipitonuchal region, quivering insertion in abdomen, quick pricking by single hand in the four limbs and back, and chop acupuncture in palm and pelma. The insertion methods are systematically discussed in terms of preparation, acupoint position, manipulation and attention, etc, so that to provide the reference for painless needle insertion.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Meridians ; Needles

Objective To study the diagnostic value of digital mammography(DM) and color Doppler ultrasonography (CDUS) for small breast carcinoma and axillary lymph nodes metastasis.Methods DM and ultrasonographic findings of 60 cases with breast nodules less than 2.0 cm in diameter were analyzed and diagnosed by experienced radiologists and ultrasonography doctors with double blind method and compared with pathological results.Results Among 60 cases, 60 breast nodules and 96 axillary lymph nodes (45/96 metastatic nodes) were both found by DM and CDUS,including 36 cases of breast cancer and 24 cases of benign lesions. The sensitivity and accuracy of DM in diagnosis of small breast cancer and axillary lymph nodes metastasis were significantly higher than that of CDUS, especially for the lesions were less than 1 cm in size (P

BACKGROUND: Iron metabolism disorder has been proved to be a key factor of Parkinson's disease, but research on brain iron metabolism is still immature, and the role of glial cells in the brain iron metabolism remains unclear. Many studies have pointed that microglia plays an important role in the pathogenesis of Parkinson's disease, which closely related to iron.OBJECTIVE: To further explore the relationship between microglia and neurons in iron metabolism based on our previous study, and to clarify the role of microglia in iron-induced selective damaged dopaminergic neurons of Parkinson's disease.METHODS: This was an in vitro cellular experiment, which was finished in the Medical College of Qingdao University, Shandong Province, China. The effects of conditioned medium of microglia on the survival rate of mesencephalic neurons and iron metabolism were observed by changing the iron levels in microglia.Lipopolysaccharide was used to activate different iron-loaded microglia, and then the effects of conditioned medium on the survival rate of mesencephalic neurons and iron metabolism were determined. The effects of different iron levels in microglia on microglia and inflammatory factors and lactoferrin released from the activated microglia were detected by siRNA and ELISA. The effects of these inflammatory factors and lactoferrin on the survival of mesencephalic neurons and iron metabolism were observed. The iron levels in mesencephalic neurons were changed, and then added into the above conditioned medium to observe the survival of neurons and iron metabolism. This experimental protocol was approved by the Hospital Ethics Committee (No. 01482311234). All experimental procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals issued by the United States National Institutes of Health.RESULTS AND CONCLUSION: Our study results show that the function of microglia is closely related to the iron metabolism in nigra dopaminergic neurons. Research on the rational use of microglia to prevent neuronal degeneration gives a strong impetus to the prevention and treatment of Parkinson's disease.

Experimental respiratory distress syndrome was induced with oleic acid injection to 8 dogs. 4 dogs received no treatment and served as controls, and the other 4 were treated with anisodamine (654-2) after oleic acid injection.All the animals were killed at the 72nd hour after injection, and their lung tissues were studied under electron microscope.It was found that anisodamine was able to reduce the severity of pulmonary edema. There was accumulation of neutrophils in the pulmonary capillaries and hyaline membrane formation on the epithelial surface of the alveoli and alveolar duct walls. Neutrophil accumulation in the capillaries may indicate that the content of the blood was kept at a fairly high level at the 72nd hour after injection in spite of anisodamine treatment. The chief constituent of the hyaline membrane was fibrin. At the sites of hyaline membrane formation, focal cyto-plasmic necrosis of the type I epithelial cells was often observed.

OBJECTIVE:To establish an HPLC method for the content determination of pseudoephedrine hydrochloride and dextromethorphan hydrobromide in pediatric pseudoephedrine dextromethorphan drops. METHODS:The separation was performed nomn .A Tghilee nftl oCw18 rcaotelu wmans wseitth a tm 0o.b6i lme Lp?hmasien -o1f amnedt hthaen oiln-j ewcatitoern- vtroieluthmynla mwainse 1(05 ?60L .∶ 4R3E0S ∶ U10L,TSp:H=Th3e. 0l)ineaat rd reatnegctei own ewrea v7e.3le4n～gt7h3 o.4f ?2g0?9 mL-1 for pseudoephedrine hydrochloride(r=0.999 9,n=5)with an average recovery of 99.1%(RSD=0.76%,n=9)and 2.55～25.5 ?g?mL-1 for dextromethorphan hydrobromide(r=0.999 9,n=5)with an average recovery of 98.0%(RSD=0.87%,n=9). CONCLUSION:The method is accurate and reproducible for quality control of pediatric pseudoephedrine dextrometharphan drops.

Objective To investigate the value of intraoperative cholangiography in laparoscopic cholecystectomy(LC). Method Intraoperative cholangiography was attempted in 1226 patients, and was successful in 1180(95.5%). Result Stones were found in the common bile duct in 78 patients, bile duct injury in seven and accessory hepatic duct in two were found.Conclusion Intraoperative cholangiography is a simply and useful procedure in providing clear imaging of biliary system, improving the success rate of LC and reducing the chance of bile duct injury.

Objective To seek a new closed reduction for anterior dislocation of shoulder with fracture of surgical neck of humerus.Method 6 cases in present study were well followed up.There were 4 males and 2 females.The average age was 42.5 years old;the average time of injury to reduction was 3.5 hours.After local anesthesia,on prone position,one hand carried on the head of humerus, another pushed the scapula.All cases were treated successfully.Result According to the Neer criterion, 4 cases were excellent,2 cases were satisfactory.Conclusion Anterior dislocation of shoulder with fracture of surgical neck of humerus can be succeeded in manual reduction.