Objective To investigate the role of the imbalance of Th 1/Th 2 cells in the pathogenesis of autoimmune thyroid diseases (AITD). Methods Thirteen patients with Graves′ disease (GD), 12 patients with Hashimoto′s thyroiditis (HT) were recruited for the study, and 8 patients with non-toxic nodular goiter served as controls. The expressions of interferon-? (IFN-?) and interleukin-4 (IL-4) of intrathyroidal mononuclear cells (ITMC), representative cytokines of Th 1 and Th 2 cells respectively, were assayed by immunohistochemical technique. Thyroid stimulating antibody (TSAb), thyroglobulin antibody (TGAb) and thyroid microsome antibody (TMAb) in peripheral blood were detected by routine methods, and their correlations with the IFN-? and IL-4 were analyzed. Results (1) Compared with the controls, the IL-4 and IFN-? expressions of ITMC in GD and HT patients were remarkably increased (both P

The pancreatic polypeptide (PP) cells of rat pancreatic islets were demonstrated by immunocytochemical method. The distribution and morphology of PP cells were investigated in order to observe their changes during postnatal development. At the lst day and 7th day of rat after birth, the PP cells of islets were different from that in adult, the processes of PP cells were not evident, and the immunoreactivity was weaker. In addition, the PP cells were also presented in the duodenum epithtlium. After 18th day, the distribution and morphology of the PP cells did not show obvious difference from that in adult. The percentage of PP cells of islets was less in lst day, and approximated to adult level in 18th day, and over adult level in 28 th day. The above changes of percentage of the PP cells showed mainly in islets in the head of pancreas. The changes of volume density and numerical density per area corresponded with the changes of percentage of the PP cells. Immunoelectron microscopic study showed that the secretory granules (SGs) and gold la belled particles on the SGs of the PP cells in 7th day were less than that in 28th day and adult. Based upon the morphology of the SGs, the PP cells can be classified into two types. The type Ⅰ cells contained SGs with medium electron density or empty-like cores, and larger SGs were more. The type Ⅱ cells contained SGs with lower electron density cores, and small SGs were more. The gold labelled particles in SGs in the type Ⅰ were more than that in the type Ⅱ. The results suggest that the PP cells of rat islets continue to change after birth.

Objective To know the heart involvement in Behet′s disease.Methods Echocardiography (UCG) and clinical characteristic evaluation were performed in 30 patients with Behet′s disease.UCG findings were compared with those in 30 control subjects.Results Valve abnormalities were common on the UCG.Valve prolapse was observed in 10% and proximal aorta dilatation in 7% of patients.The positive rate of antinuclear antibodies was very low (7%) without differences between both groups.Conclusion Heart valve diseases are common in patients with Behet′s disease.

Agarases are glycoside hydrolases.They are grouped into?and?types,which hydrolyze?-1,3 linkages and?-1,4 linkages respectively.The paper is about advance in research of agarase including the research of biology,the classcification,the crystal structure,the catalysis mechanism and application of agarases.

OBJECTIVE:To observe clinical efficacy and safety of Astragalus injection combined with neoadjuvant chemother-apy and three dimensional conformal radiotherapy in the treatment of colorectal cancer. METHODS:A total of 80 patients with me-dium and advanced colorectal cancer were selected from oncology department of our hospital during Jun. 2010 to Jun. 2014,and di-vided into observation group and control group according to random number table,with 40 cases in each group. Control group re-ceived neoadjuvant chemotherapy combined with three-dimensional conformal radiotherapy. Observation group was additionally giv-en Astragalus injection 10-20 mL added into 5％ Glucose injection 250-500 mL,ivgtt,qd,on the basis of control group. A treat-ment course lasted for 21 d,and both groups received 3 courses. Short-term efficacies as well as immune indexes [CD3+,CD4+, CD4+/CD8+ and NK cells] before and after treatment were observed in 2 groups. The occurrence of ADR and survival situation were compared between 2 groups. RESULTS:Clinical response rate of observation group was 87.5％,which was slightly higher than that of control group(77.5％),but there was no statistical significance(P>0.05). Before treatment,there was no statistical signifi-cance in immune index levels between 2 groups(P>0.05). After treatment,NK cells,CD3+,CD4+ and CD4+/CD8+ levels of con-trol group were decreased significantly,which was significantly lower than observation group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). Two-year survival rate of observa-tion group was significantly higher than that of control group;the recurrence rate and distant metastasis rate were significantly low-er than control group,with statistical significance(P<0.05). CONCLUSIONS:Astragalus injection not only can improve therapeu-tic efficacy of neoadjuvant chemotherapy in patients with colorectal cancer,balance inflammatory state,improve survival rate,re-duce recurrence rate and distant metastasis rate with good safety.

Objective:To explore the value of the elasticity contrast index (ECI) in differential diagnosis of thyroid nodules, and contrast the effect of 4 different methods on measuring ECI.Methods:A total of 122 patients with 131 thyroid nodules in Binzhou Medical University Hospital from May 2018 to May 2019 were enrolled, and elastography was performed in 4 different ways such as in axial plane for internal (AI), axial plane for periintranodular (AP), longitudinal plane for internal (LI) and longitudinal plane for periintranodular (LP). The cut-off values for predicting malignant nodules in 4 different ways were determined separately using receiver operating characteristic (ROC) curve analysis.Results:There were 54 benign and 77 malignant ones in 131 nodules. The ECI in AI, AP, LI and LP in benign thyroid nodules was significantly lower than that in malignant ones: 2.10 (1.48, 2.34) vs. 3.07 (2.73, 3.87), 1.91 (1.64, 2.18) vs. 2.62 (2.24, 3.07), 2.19 (1.59, 2.39) vs. 3.00 (2.72, 3.63) and 1.89 (1.71, 2.16) vs. 2.66 (2.21, 3.10), and there was statistical difference ( P<0.05). Among the 4 different ways, the largest area under curve was achieved in AI with 0.925, and the corresponding optimal diagnostic threshold was 2.43 (with 90.9% sensitivity, 87.0% specificity, 88.5% accuracy, 89.7% positive predictive value and 86.8% negative predictive value). Conclusions:ECI is helpful for conventional ultrasound to diagnose thyroid nodules as malignant or benign. The best value is obtained in AI.

Female ; Humans ; Infant ; Mastocytosis, Systemic ; diagnosis ; pathology ; therapy

Objective To study effect of the antisense oligodeoxynucleotide (ASODN) of small subunit of human ribonucleotide reductase (RRM2) mRNA on cell line of human choriocarcinoma in vitro. Methods Two 20-mer gapmer ASODNs with a full phosphorothioate backbone were artificially synthesized, which were complementary to nucleotides 626-645 (a coding region) and 1572-1591 (a 3′untranslated region) of RRM2, respectively. ASODNs were transfected into JAR cells through oligofectamine. The survival rate was assessed by methyl thiazolyl tetrazolium (MMT) assay, and RRM2 expression was detected by immunoblot and reverse transcriptase polymerase chain reaction (RT-PCR) methods. Results Antisense oligodeoxynucleotide one (ASODN1) targeting the coding region significantly inhibited growth of JAR cells in a dose- and time-dependent manner and downregulated RRM2 expression in a time-dependent manner. ASODN1 at 100 nmol/L could inhibit significantly cell growth ( P =0.000), and the effects of ASODN1 on JAR cell proliferation were enhanced with increase of ASODN1 concentration and reached the peak point at 400 nmol/L concentration ( P =0.000). Cell growth was significantly inhibited by 200 nmol/L of ASODN1 after 24 h of treatment ( P =0.000). The effect of ASODN1 was at the maximum at 48 h ( P =0.000), and began to decrease at 72 h of treatment. RRM2 expression started to reduce after ASOND1 treatment for 12 hours, and was obviously downregulated at 24 h of treatment, and decreased to the lowest level at 48 h ( P

The high frequency subject terms were extracted from the PubMed-covered papers published from January 2000 to March 2015 on text mining of biomedical field to generate the matrix of high frequency subject terms and their source papers.The co-occurrence of high frequency subject terms in a same paper was analyzed by clustering analysis.The hotspots in text mining of biomedical field were analyzed according to the clustering analysis of high frequency subject terms and their corresponding class labels, which showed that the hotspots in text mining of bio-medical field were the basic technologies of text mining, application of text mining in biomedical informatics and in extraction of drugs-related facts.

Objective To evaluate the utility of carbapenem inactivation method (CIM) in detecting carbapenemase-producing Acinetobacter baumannii.Methods A total of 121 strains of A. baumannii were identified and subjected to antimicrobial susceptibility testing by VITEK compact. Carbapenem inactivation method (CIM) was applied to detect the carbapenemase in the A. baumannii strains. The OXA-23 type carbapenemase-encoding genes were analyzed by common PCR method.Results Six-eight of the 121 strains showed resistance to imipenem and meropenem. PCR showed that 65 of the 68 strains carried OXA-23 gene. CIM was positive in 66 of the 68 strains. And 52 of the 121A. baumannii strains were susceptible to imipenem and meropenem. PCR showed that OXA-23 gene was negative in 49 of the 52 strains. CIM was negative in the 52 strains of non-carbapenemase-producing A. baumannii. Only one strain was resistant to imipenem but susceptible to meropenem. CIM was negative but QXA-23 was positive for this strain. The sensitivity and the specificity of CIM was 94.2% and 98.1% respectively in detecting carbapenemase-producing A. baumannii.Conclusions The results of CIM were consistent with the results obtained by PCR to detect the encoding gene of OXA-23. CIM is inexpensive, easier to operate and interpret than PCR method. CIM is applicable to detect OXA-23 type carbapenemase rapidly inA. baumannii.