50 tracheotomy patients with mechanical breathing were performed respiratory tract control for suitable breathing. Result 5 patients recovered and had spontaneously breathing without respirator in 3~5days, then the trachea cannulas were pulled out in 1~2 weeks. Conclusion effective respiratory tract control may enhance the curative rate and improve patients' life quality.

Phylogenetic markers are the gene fragments that demonstrate the gene tic relationships between organisms To differentiate close taxa, many new phyl oge n etic markers were used, with which the polyphasic taxonomy was enriched This a r ticle chiefly described the characters of several phylogenetic markers and their applications in the studies of the phylogenetics of bacteria

Medical purpose is a concept of viewing the situation as a whole, development and perpetuity. It is important that how to realize the medical purpose but not define itself.There are similar main conten of medical purpose extending directly from various stages and existing between eastern and western countries, and this is determined by characteristics of mde-icine. However, the fommer concept of medical purpose will show relative partial deficiency, and its intension will enlarge in the new stage of social, ecconomic and medical development. It also brings us new methods, ideas of thought, and make them fit for the requirement of society to medicine and the non-fungible function of medicine to human health. So the central content of medical purpose is running after the health of person and human based on elevating life quality, maintain and enhance social productive forces. This is extreme purpose of medicine and common adimission of world medicine.The realization of medical purpose is guided and restricted by the concept of humanistic ethics. So, understanding and discussion of medical bepose must be carried out guided by it and its theory frame. The centre of mordern medical humanism is the respection to the human valuation related to medicine, including that of person, group and society. Primarily, the valuation is emphasized on the human life quality and health valuvation, which are directly and first reflected not only in mordern medical humanism, but also in medical purpose.In this sense, the intension of medical humanism is equivalent to the central content of medical prupose defined above all, and the medical humanism is medical purpose. The focus reflection of medical purpose is the thought of medical humanism, one is objective of medical developmant another is the thought and principle defined for achieving the objective.

Objective: To study the chemical constituents of Chinese herb Fructus Ligustri lucidi , providing theoretic evidence for its exploitation and utilization. Methods: Chemical constituents of Fructus Ligustri lucidi were isolated and purified by solvent extraction and chromatography; their structures were identified by physiochemical properties and spectroscopic analysis. Results: Seven compounds were isolated from the ethanol extract of the fruits and identified as oleanolic acid(Ⅰ), luteolin 7 O ? D glucoside(Ⅱ), quercitrin(Ⅲ), ? sitosterol 3 O ? D glucoside(Ⅳ), p hydroxyphenethyl ? D glucoside(Ⅴ), D mannitol(Ⅵ) and hexitol(Ⅶ). Compounds Ⅱ,Ⅲ and Ⅶ were obtained from this plant for the first time. Conclusion: Seven compounds have been obtained from the title plant, which provides a base for its future use.

The pterion of 400 Chinese adult skulls, 252 males and 148 females, wasstudied. It was found that the epipteric bone was rather common. When cal-culated according to A. J. P. von Broek's classification which includes epiptericumanterius, epiptericum posterius, in addition to the most common epiptericumtypicum, the frequency was 42% in terms of number of skulls ed 27% interms of number of sides. The fronto-temporal suture type, however, was smallin number (3.0% in terms of the number of skulls and 1.75% in terms of thenumber of sides), due to the presence of a frontal process of the temporalbone. Both the epipteric bone and the fronto-temporal suture were found to bemore common in the female than in the male. Various theories regarding theorigin of these alterations are introduced and discussed.

ApolipoptoteinA1isthemaincomponentofhigh-densitylipoproteincholesterolintheplas-ma,which plays an important role in the lipid transportation and metabolism.It is closely related with cardio-vascular disease,liver disease,hereditary amyloidosis and so on.CA1 25 ,as an important biomarker,has been widely used for the diagnosis and monitoring treatment of ovarian cancer.Improved sensitivity and specificity of diagnosis are obtained when it is combined with other tumor markers,especially CA1 25 .As reported recently, it also is related with the generation,progress and prognosis of ovarian cancer.It has been promising to be a new kind of treatment.

ObjectiveTo testify the therapeutic efficacy of acupuncture in treating diarrhea-predominant irritable bowel syndrome (D-IBS) based on brain-gutinteraction.MethodEighty D-IBS patients were randomized into brain-gut interacted acupuncture group (treatment group) and a group with acupoints selected based on conventional syndrome differentiation (control group), 40 in each group. The treatment group was intervened by acupuncture by selecting the thirteen ghost acupoints (invented by SUN Si-miao) plus Tianshu (ST25), Dachangshu (BL25), etc., while the control group was by acupuncture based on conventional point selection, including Zusanli (ST36),Tianshu (ST25), Dachangshu (BL25), Ganshu (BL18), Pishu (BL20), etc., three times a week, and 10 sessions as a treatment course. The clinical efficacies were evaluated after 2 treatment courses.ResultThe two groups were generally equal to each other incomparing each dimension of the Short-form Health Survey (SF-36) before treatment, while the scores were increased after treatment in both groups; the treatment group was significantly superior to the controlgroup in comparing the scores ofrole-physical,bodily pain, general health, vitality, social role functioning, and reported health transition from the SF-36 (P<0.05).ConclusionAcupuncture based on brain-gut interaction can produce a more significant effect in treating D-IBS compared to conventional acupuncture.

Objective To observe the effect of penetrating needling with thick needle at Shenzhu (GB 12) on the Unified Parkinson’s Disease Rating Scale (UPDRS) score in patients with Parkinson’s Disease (PD). Methods Sixty-one PD patients were randomized into a treatment group of 31 cases and a control group of 30 cases. The treatment group was intervened by penetrating needling with thick needle at Shenzhu (GV 12) in addition to oral administration of Levodopa and Benserazide Hydrochloride;while the control group was by oral administration of Levodopa and Benserazide Hydrochloride alone. The UPDRS was evaluated before and after intervention in both groups. Results Respectively after 30-day treatment, 90-day treatment, and 90 d after the intervention, the UPDRS scores were significantly changed compared to that before intervention in both groups (P<0.01, P<0.05);there were also significant differences in comparing the UPDRS score between the two groups at each time point (P<0.01, P<0.05). Conclusions Penetrating needling with thick needle at Shenzhu (GV 12) can improve the UPDRS score in PD patients, and it’s an effective method in treating PD.

Hereditary diffuse gastric cancer (HDGC) is an autosomal dominant inherited disease,and may be related to the mutation of CDH1 or CTNNA1 genes.Microscopically,signet-ring cell carcinoma is suggested frequently in endoscopic biopsy or gastrectomy specimens.Some patients may have concomitant extra-stomach tumor (frequently breast cancer in females).Detection of CDH1 gene mutation should be performed in high-risk individuals,and diagnosis and treatment should be carried out by a multidisciplinary team.Prophylactic gastrectomy is recommended for those with pathogenic CDH1 mutation.Endoscopic surveillance is an option for those with CDH1 mutation of undetermined significance and those without germline CDH1 mutation.This review discussed the concept,genetic characteristics,clinicopathological features and genetic screening of HDGC for providing a reference for clinicians.

OBJECTIVE:To establish a method for the contents determination of volatile components in Huodan pill and study the effects of 60Co irradiation with different doses on the volatile components in Huodan pill. METHODS:GC-MS was used to de-termine the contents of patchouli alcohol and the ralative contents of β-patchouliene,α-guaiacene,seychellesene,α-patchouliene,α-bulnesene,5,11-diene guaiacyl,and unidentified objects in Huodan pill after 60Co irradiation with different doses. Column was HP-5MS by programmed temperature,volume temperature was 250℃,split ratio was 50:1,detector was triple quadrupole mass spectrometer detector,MS1 quadrupole temperature was 150℃,MS2 was 150℃,carrier gas was helium,flow rate of column was 1.2 ml/min,and the volume injection was 1 μl;ion source was EI,bombarding energy was 70 eV,temperature of ion source was 230℃,temperature of transmission line was 280℃ and scan range was 50-500 amu. RESULTS:The linear range of patchouli alcohol was 0.103 1-2.062 0μg(r=0.999 4);RSDs of precision,reproducibility and stability tests were lower than 1.0%;recovery was 98.05%-102.32%(RSD=1.8%,n=9). 60Co irradiation with different doses had little effects on the 8 volatile components in Huodan pill. CONCLUSIONS:60Co irradiation can be used for sterilization on Huodan pill,but irradiation dose should be controlled. The method is simple,good reproducibility,and can be used for the contents determination of volatile components in Huodan pill.