ObjectIve To InvestIgate the balance between cerebral O2 supply and consumptIon and glucose metabolIsm durIng supratentorIal tumor resectIon under total Intravenous anesthesIa (TIVA) wIth propofol. Methods Twenty-four patIents of ASA grade Ⅰ- Ⅱ aged 20-55 undergoIng electIve supratentorIal tumor resectIon were randomly dIvIded Into 2 groups : lIdocaIne group (A, n = 12) and control group (B, n - 12) . After sedatIon wIth Intravenous scopolamIne 0.3 mg, mIdazolam 0.02 mg?kg-1 and droperIdol-fentanyl mIxture 0.03 ml? kg-1 radIal artery was cannulated for Bp monItorIng and blood samplIng and left Internal jugular veIn was cannulated retrogradely and the catheter was advanced cephalad untIl jugular bulb for blood samplIng. In lIdocaIne group anesthesIa was Induced wIth lIdocaIne 1.5 mg?kg1, propofol 2 mg?kg1, droperIdol-Ientanyl mIxture 0.04 ml?kg-1 and pIpecuronIum 0.15 mg ? kg1 and maIntaIned wIth propofol InfusIon at 6-8 mg?kg-1?h-1 and IntermIttent I. v. boluses of fentanyl and pIpecuronIum. LIdocaIne was Infused after InductIon of anesthesIa at 11.7 mg ?kg1? h-1 for 20 mIn then InfusIon was maIntaIned at 2 mg ?kg1? h-1 .In control group lIdocaIne was replaced by normal salIne. The patIents were mechanIcally ventIlated (VT 6-8 ml?kg1 , RR 12-14 bpm) and PETCO2 was maIntaIned at 29-31 mm Hg In both groups. Blood volume and hemodynamIcs were well maIntaIned durIng operatIon. Blood samples were taken from artery (a) and jugular venous bulb (jv) sImultaneously before InductIon of anesthesIa (T0, baselIne), at IntubatIon (T1), whIle tumor was beIng Isolated (T2) and resected (T3) and at the closure of dura (T4 ) , for blood gas analysIs and determInatIon of Hb and glucose and lactate concentratIon. The dIfference In arterIal and jugular bulb O, content ( Ca-jvO2 ) , cerebral O2 extractIon rate (O3ER), glucose extractIon rate ( GER) and lactate productIon rate (LacPR) were calculated. Results The two groups were comparable wIth respect to demographIc data, duratIon of operatIon and the amount of propofol and fentanyl used. SjvO2 was sIgnIfIcantly lower, Ca-jvO2 greater and O2ER hIgher at T1 In group A than In group B. There was no sIgnIfIcant dIfference In GER and LacPR between the two groups. ConclusIon A bolus of lIdocaIne 1.5 mg?kg-1can increase cerebral O2 extraction during induction and intubation but lidocaine infusion at 2 mg?kg-1?h-1 has no significant effect on cerebral O2 supply/demand and glucose metabolism.

Objective:To observe influence of preoperative sufentanil delayed preconditioning (SPC) on perioperative cogni‐tive function and myocardial protection effect in patients undergoing on -pump coronary artery bypass grafting (OP‐CABG) .Methods:A total of 60 patients undergoing selective OPCABG were randomly and equally divided into sufentanil group (SPC group) and routine treatment control group (control group) ,blood sample was collected before operation ,1h , 6h ,12h ,24h and 48h after aorta declamping to measure level of cardiac troponin I (cTnT );and all patients received neu‐ropsychological assessment before and 7d after operation .Results:Compared with control group ,there was significant re‐ductions in serum cTnI levels [1h:(23.66 ± 3.15) ng/ml vs .(14.96 ± 2.13) ng/ml] in sufentanil group from 1h after aor‐ta declamping to 48h after CABG ,P<0.05 all .The inotropic agent usage score on 12h ,24h and 48h after entering ICU of sufentanil group was significantly lower than that of control group [12h:(6.4 ± 0.6) scores vs .(8.4 ± 0.6) scores] , P<0.05 all .Incidence rate of postoperative cognitive dysfunction (POCD) in sufentanil group was significantly lower than that of control group (13.3% vs .30.0% ,P=0.041) .Conclusion:Preoperative sufentanil delayed preconditioning posses‐ses myocardial protection effect , and reduce incidence rate of cognitive dysfunction so also possesses certain brain protection in patients undergoing on-pump coronary artery bypass grafting .

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.
Amino Acid Sequence ; Animals ; Base Sequence ; Cats ; *Genetic Variation ; Goats ; Humans ; Molecular Sequence Data ; Phylogeny ; Protozoan Proteins/chemistry/*genetics/metabolism ; Sequence Alignment ; Sheep ; Superoxide Dismutase/chemistry/*genetics/metabolism ; Toxoplasma/classification/*enzymology/genetics/isolation & purification ; Toxoplasmosis/*parasitology ; Toxoplasmosis, Animal/*parasitology

Objective To analysis the electroencephalographical (EEG)specificity of psychological adjustment of Imaginary Working (IW)by comparing the EEG power of trained subjects and untrained subjects.Methods University students were recruited as the subjects,randomly and equally divided into experimental group and control group.The students of experimental group were trained Imaginary Working,with no intervention on those of control group.The changes of powers during the process of Imaginary Working were synchronously detected by EEG,furthermore,the delta(δ)band,theta(θ) band,alpha(α)band,and beta (β)band were compared and analyzed from three aspects of general points,time interval,and different leads.Results There were very significant differences in δ,θ,αand βbands between the two groups at both Imaginary Working (IW)and resting state (RS)(P <0.001):the absolute power of δband in experimental group was lower than that of control group,and the absolute powers of θ,αand βbands were higher than those of the control group.There were significant differences of absolute power of δband at RS0 ,IW1 ,IW2 and RS3 (P <0.05),and there were significant differences of θband at RS 1 ,RS2 and IW3 (P <0.01 ).The changes of absolute power of δand θbands in experimental group were close to cosine curves.Although there were statistical differences of αband and βband at each state,from IW1 period the amplitude of αband began to keep stable,and from IW2 period the amplitude of βband did.There were no significant differences of δband and θband appeared different leads between the two group(P >0.05).The absolute power of αband showed statis-tical differences in lead of F7,C3,C4,P3,P4,T3,T4,T5,T6,PZ,O1 and O2(P <0.05),while that of βband showed significant difference in lead of FP2,F7,F8,P3,P4,T5,T6,C4,PZ,O1 and O2 (P <0.05).The highest power of αand βband both appeared in the lead of O1 and O2.Conclusion Imag-inary Working induced the rhythmic response of δ,θ,αand βband,especially the rhythm alternated fast waves and slow waves.It is suggested that Imaginary Working keep the brain in rest state,and relieve tension,and adjust negative emotions.

Objective To analyze the effects of different kinds of Image Thinking on brain functional area by measuring alpha band electroencephalograph (EEG) power.Methods 48 college students were enrolled and divided into two groups .Subjects in the experiment group received three kinds of Image Thinking intervention , while those in the control group did not receive any intervention .Alpha band ab-solute power of two groups was measured to compare and analyze .Results The alpha band absolute power value of the experiment group was higher than that in the control group at occipital lobe , parietal lobe , and temporal lobe in both active and resting state ( P<0 .05 ) .The power in αfrequency range was significantly different in three kinds of Image Thinking status.When imagining“I am rambling in the rose bushes”, there was statistically significant difference in contralateral lead in ( FP1-FP2 ) , ( F7-F8 ) , (C3-C4), (P3-P4), (T5-T6), and (O1-O2) (P<0.05).Moreover, power in the right hemisphere was higher than left hemisphere , and after active stage the power of occipital lobe reduced .When imagi-ning “the spring sunshine is warm on my body”“My every pore is breathing freely”, there was statistically significant difference in contralateral lead in (F7-F8) and (P3-P4) (P<0.05).The alpha band abso-lute power increased at occipital lobe after active stage , then the power increased to peak at final resting state.Conclusion Image thinking exhibits a significant tranquilizing effect according to the reflection of the high power of alpha band at occipital lobe and parietal lobe .Different kinds of feeling effect caused by Image Thinking maybe related to the function of every brain area .

Objective To observe the stability of orthostatism and weight-bearing position,and range of motion (ROM) of vertical plane and coronal plane of thoracic vertebrae through measuring spinal forms before and after standing exercise,and explore the corrective effect of standing exercise on spine unbalance.Methods The university students (n =30) were chosen as subjects,and divided into test group and control group.After standing exercise,the changes of thoracic intervertebral angles of spinal orthostatism and weight-bearing position,anterior flexion and posterior extension of vertical plane,and left bending and right bending of coronal plane were measured by using spinal measurement apparatus in 2 groups.The measurement results were compared.Results The comparison results showed that the stability of 2 spinal positions and ROM of vertical plane and coronal plane had significant differences between 2 groups (P ＜ 0.05).The ROM of coronal plane had greatly significant difference in test group before and after standing exercise (P ＜0.001),and that has no difference in control group at the same time points.Conclusion The differences in the stability and ROM of thoracic vertebrae indicate that standing exercise can adjust the form of spine and correct spine unbalance.

Objective To explore the tranquilizing effects of Imaginary Working by investigating the timing characteristics of the alpha band electroencephalogram ( EEG ) power. Methods College students as volunteer subjects were recruited and randomly divided into experimental group and control group at 1∶1 ra-tio. The experimental group received Imaginary Working intervention while the control group did not re-ceive any intervention. After Imaginary Working intervention training, the alpha band absolute power of two groups was measured to compare and analyze. Results Alpha band EEG absolute power in the two groups were statistically different at all time periods (P<0. 05). The absolute power value of the experi-mental group was higher than the control group, appeared more steady than the control group at each time point. Conclusion Imaginary Working can induce the alpha band rhythm response and maintain the effects with time as a subsequent effect. Imaginary Working presents with an apparent tranquilizing effect.

Objective To observe whether there was difference of inferior glenohumeral joint capsule thickness(ICT)measured on coronal and transverse axillary section with high-frequency ultrasound.Methods ICT of 56 patients with frozen shoulder(FS group)and 115 healthy controls(HC group)were measured on coronal and transverse axillary sections with high-frequency ultrasound.The ultrasonic findings were compared between groups,while ICT measured on different sections were compared within groups.Results In FS group,ICT thickened,presented as low echo with poor boundary clarity,with reduced and uneven internal echo.No echo areas could be detected when there was fluid accumulation,and concomitant blood flow signal could be observed.In HC group,the inferior glenohumeral joint capsule presented as moderate echo with clear boundary,with uniform low or equal echo.No significant difference of ICT values measured on coronal or transverse section was found within both groups(both P＞0.05).Conclusion ICT measured on coronal and transverse axillary section with high-frequency ultrasound were not significantly different.

Objective To analyze and explore the gender-dependent differences of the psychological adjustment induced by baduanjin(eight-trigram boxing)by comparing the total and spatial changes of β1 and β2 electroencephalogram(EEG)power among male and female college students before and after per-forming baduanjin.Methods College students were recruited as volunteers,and were divided into male and female groups.All volunteers received baduanjin training.β1 and β2 EEG power of two groups was measured and compared with NT9200 electroencephalograph before and after performing baduanjin.The results were collected to analyze the differences of absolute power in two groups before and after baduanjin training.Results General comparison:the absolute power of the β1 and β2 band in the female group was significantly higher than that in the male group before training (P >0.05).The absolute power of the β1 and β2 band increased significantly in the male group after training (P >0.01).There was no sig-nificant difference in the female group before training(P >0.05).In addition,there was no significant difference between male group and female group in the absolute power of the β1 and β2 band after train-ing (P >0.05).Spatial comparison:there were significant differences in the distribution of β1 and β2 bands on the EEG between male and female groups before training.Compared with the distribution of β1 and β2 in different groups before training,the distribution of β1 on different groups tended to be consist-ent after training.The distribution of β2 band presented an increasing tendency on the whole brain region after training,while the female group presented decreasing tendency on most brain regions after training. Function coordination of left and right cerebral hemispheres:compared with EEG before training,the cor-relation between right and left cerebral hemispheres shown on β1 and β2 band increased significantly or changed from negative to positiv correlation.Conclusion Psychological adjustment may be induced by baduanjin,and it has the effects of removing differences of physiological movement between male and fe-male volunteers.In other words,when students perform baduanjin,mental and physiological movement in brain presents with similar pattern.