ObjectIve To InvestIgate the balance between cerebral O2 supply and consumptIon and glucose metabolIsm durIng supratentorIal tumor resectIon under total Intravenous anesthesIa (TIVA) wIth propofol. Methods Twenty-four patIents of ASA grade Ⅰ- Ⅱ aged 20-55 undergoIng electIve supratentorIal tumor resectIon were randomly dIvIded Into 2 groups : lIdocaIne group (A, n = 12) and control group (B, n - 12) . After sedatIon wIth Intravenous scopolamIne 0.3 mg, mIdazolam 0.02 mg?kg-1 and droperIdol-fentanyl mIxture 0.03 ml? kg-1 radIal artery was cannulated for Bp monItorIng and blood samplIng and left Internal jugular veIn was cannulated retrogradely and the catheter was advanced cephalad untIl jugular bulb for blood samplIng. In lIdocaIne group anesthesIa was Induced wIth lIdocaIne 1.5 mg?kg1, propofol 2 mg?kg1, droperIdol-Ientanyl mIxture 0.04 ml?kg-1 and pIpecuronIum 0.15 mg ? kg1 and maIntaIned wIth propofol InfusIon at 6-8 mg?kg-1?h-1 and IntermIttent I. v. boluses of fentanyl and pIpecuronIum. LIdocaIne was Infused after InductIon of anesthesIa at 11.7 mg ?kg1? h-1 for 20 mIn then InfusIon was maIntaIned at 2 mg ?kg1? h-1 .In control group lIdocaIne was replaced by normal salIne. The patIents were mechanIcally ventIlated (VT 6-8 ml?kg1 , RR 12-14 bpm) and PETCO2 was maIntaIned at 29-31 mm Hg In both groups. Blood volume and hemodynamIcs were well maIntaIned durIng operatIon. Blood samples were taken from artery (a) and jugular venous bulb (jv) sImultaneously before InductIon of anesthesIa (T0, baselIne), at IntubatIon (T1), whIle tumor was beIng Isolated (T2) and resected (T3) and at the closure of dura (T4 ) , for blood gas analysIs and determInatIon of Hb and glucose and lactate concentratIon. The dIfference In arterIal and jugular bulb O, content ( Ca-jvO2 ) , cerebral O2 extractIon rate (O3ER), glucose extractIon rate ( GER) and lactate productIon rate (LacPR) were calculated. Results The two groups were comparable wIth respect to demographIc data, duratIon of operatIon and the amount of propofol and fentanyl used. SjvO2 was sIgnIfIcantly lower, Ca-jvO2 greater and O2ER hIgher at T1 In group A than In group B. There was no sIgnIfIcant dIfference In GER and LacPR between the two groups. ConclusIon A bolus of lIdocaIne 1.5 mg?kg-1can increase cerebral O2 extraction during induction and intubation but lidocaine infusion at 2 mg?kg-1?h-1 has no significant effect on cerebral O2 supply/demand and glucose metabolism.

Objective:To observe influence of preoperative sufentanil delayed preconditioning (SPC) on perioperative cogni‐tive function and myocardial protection effect in patients undergoing on -pump coronary artery bypass grafting (OP‐CABG) .Methods:A total of 60 patients undergoing selective OPCABG were randomly and equally divided into sufentanil group (SPC group) and routine treatment control group (control group) ,blood sample was collected before operation ,1h , 6h ,12h ,24h and 48h after aorta declamping to measure level of cardiac troponin I (cTnT );and all patients received neu‐ropsychological assessment before and 7d after operation .Results:Compared with control group ,there was significant re‐ductions in serum cTnI levels [1h:(23.66 ± 3.15) ng/ml vs .(14.96 ± 2.13) ng/ml] in sufentanil group from 1h after aor‐ta declamping to 48h after CABG ,P<0.05 all .The inotropic agent usage score on 12h ,24h and 48h after entering ICU of sufentanil group was significantly lower than that of control group [12h:(6.4 ± 0.6) scores vs .(8.4 ± 0.6) scores] , P<0.05 all .Incidence rate of postoperative cognitive dysfunction (POCD) in sufentanil group was significantly lower than that of control group (13.3% vs .30.0% ,P=0.041) .Conclusion:Preoperative sufentanil delayed preconditioning posses‐ses myocardial protection effect , and reduce incidence rate of cognitive dysfunction so also possesses certain brain protection in patients undergoing on-pump coronary artery bypass grafting .

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.
Amino Acid Sequence ; Animals ; Base Sequence ; Cats ; *Genetic Variation ; Goats ; Humans ; Molecular Sequence Data ; Phylogeny ; Protozoan Proteins/chemistry/*genetics/metabolism ; Sequence Alignment ; Sheep ; Superoxide Dismutase/chemistry/*genetics/metabolism ; Toxoplasma/classification/*enzymology/genetics/isolation & purification ; Toxoplasmosis/*parasitology ; Toxoplasmosis, Animal/*parasitology