Objective: To study the effect of chronic intermittent hypoxia on cognitive functions in Sprague-Dawley rats and the expression of Insulin-like Growth Factor-1 in CA1 region of hippocampus.Methods: Experiments were performed on 16 three-month-old male Sprague-Dawley rats,They were randomly assigned to two experimental groups(n=8/group): chronic intermittent hypoxia group(CIH),and unhandled control group(UC).Rats in UC group were raised normally,and those in CIH group suffered 7H intermittent hypoxia everyday,The duration of experiment was 8 weeks.The learning and memory ability of rats in each group were assessed with the Morris water maze test at the end of the experiments.The expression of Insulin-like Growth Factor-1 in CA1 region of hippocampus was investigated by immunohistochemistry staining and image analysis.Results: ①Morris water maze test learning scores(place navigation): After 5 days training,the escape latency in CIH rats was significantly longer than that in UC rats(P

The experience in the management of 108 cases of deeply situated meningiomas is presented. Complete removal of the tumor has been possible in 83 cases (76.9%), partial resection in 22 cases (20.3%). The surgical results were excellent in 72.2% and fair in 16.7%. Early diagnosis and an attempt to remove completely of such tumors should always be considered as the treatment of choice. In order to reach this goal, we propose:(a)wide exposure, induced hypotension, hypothermia, interruption of the blood supply at the base of the tumor, and careful hemos-tasis are essential; (b) in addition, microsurgical dissection to avoid injury of the neighbouring important structures as well as vital areas of the brain are mandatory. The removal of an unusual giant mening-ioma arising in the medial aspect of the sphenoid ridge illustrated these points well. The tumor was13?10?8cm in size and 864g in weight. The patient recovered uneventfully and his general condition has remained good for years. Recent CT scan showed no recurrence.

Objective:To investigate the correlation between heart rate recovery at 1 min after exercise (HRR 1) and long-term prognosis in patients with acute myocardial infarction treated by percutaneous coronary intervention (PCI). Methods:The clinical data of 117 acute myocardial infarction patients who successfully underwent PCI in Qingdao Starr Heart Hospital from May 2014 to May 2017 were retrospectively analyzed. The patients were performed cardiopulmonary exercising test within 30 d after PCI, the peak heart rate (HR peak), resting heart rate, peak oxygen uptake (VO 2peak) were detected, and the HRR 1, heart rate reserve and oxygen pulse were calculated. The correlation between HRR 1 and cardiopulmonary exercise related indexes was analyzed. The incidence of major adverse cardiovascular events (MACE) was recorded 3 years after PCI. The risk factors affecting the prognosis in patients with acute myocardial infarction were analyzed by Cox regression. Results:Among 117 patients, HRR 1 didn′t decrease in 74 cases (non-reduced group), and HRR 1 decreased in 43 cases (reduced group). The proportion of Killip heart function grade Ⅱ to Ⅲ, proportion of triple coronary artery lesions, Gensini score, resting heart rate and oxygen pulse in reduced group were significantly higher than those in non-reduced group: 90.7% (39/43) vs. 70.3% (52/74), 53.5% (23/43) vs. 17.6% (13/74), (81.46 ± 21.55) scores vs. (58.06 ± 17.52) scores, (81.48 ± 11.17) times/min vs. (69.06 ± 10.10) times/min and (13.13 ± 2.93) times/min vs. (9.19 ± 3.06) times/min, the VO 2peak, HR peak, HRR 1 and heart rate reserve were significantly lower than those in non-reduced group: (20.19 ± 9.39) ml/(kg·min) vs. (26.38 ± 9.40) ml/(kg·min), (124.98 ± 22.79) times/min vs. (149.47 ± 22.88) times/min, (16.63 ± 5.92) times/min vs. (27.24 ± 10.21) times/min and (55.51 ± 10.29) times/min vs. (69.07 ± 12.27) times/min, and there were statistical differences ( P<0.01). Correlation analysis results showed that HRR 1 was positively correlated with heart rate reserve, HR peak, VO 2peak ( r = 0.793, 0.835 and 0.800; P<0.01), and negatively correlated with resting heart rate ( r = -0.748, P<0.01); but there was no correlation between HRR 1 and oxygen pulse ( P>0.05). Twenty-one patients did not complete 3 years′ follow-up, with 8 cases in reduced group and 13 cases in non-reduced group. The incidence of MACE in reduced group was significantly higher than that in non-reduced group: 62.9% (22/35) vs. 13.1% (8/61), and there was statistical difference ( χ2 = 12.546, P<0.01). Multivariate Cox regression analysis result showed that the number of coronary artery lesions and HRR 1 were independent risk factors affecting the prognosis in patients with acute myocardial infarction ( HR = 3.124 and 0.248, 95% CI 1.044 to 9.342 and 0.080 to 0.771, P<0.05). Conclusions:The reduction of HRR 1 is related to the exercise cardiopulmonary index in patients with acute myocardial infarction, which can be used as one of the important predictors of long-term prognosis of patients.

BACKGROUND:BioFlex system as a new pedicle screw fixation of dynamic stabilization device has less been reported concerning its biomechanics.
OBJECTIVE:To study the effect of BioFlex system fixation at different decompression ranges on disc stress at adjacent segments.
METHODS:Eight samples of fresh calf spines were used. Under physiologic axial loads (500, 900, 2 300 N), electronic universal testing machine was used to simulate the lumbar spine at three physiological states (standing, sitting and bending, standing on a portable 20 kg weight and bending). Progressive decompression modeling for each specimen and dividing into five groups:(1) complete status group;(2) complete status+BioFlex group;(3) partial laminectomy+BioFlex group;(4) 1/2 medial facetectomy+BioFlex group;(5) total facetectomy+BioFlex group. Strain gauges were used to record the stress of disc annulus. Electronic universal testing machine was used to record load-displacement curve and calculate stiffness.
RESULTS AND CONCLUSION:(1) The stress of the adjacent segment of the intervertebral disc increased with the expansion of the range of decompression. Compared with the complete status, stress obviously increased after BioFlex fixation, showing significant differences (P<0.05). The stress was significantly increased in the 1/2 medial facetectomy+BioFlex group compared with the partial laminectomy+BioFlex group (P<0.05). However, no significant difference was detected between the partial laminectomy+BioFlex group and complete status+BioFlex group, and between total facetectomy+BioFlex group and 1/2 medial facetectomy+BioFlex group (P>0.05). (2) Axial stiffness reduced with the expansion of the range of decompression. Compared with the complete status, axial stiffness noticeably increased after BioFlex fixation. The difference was not significant among four kinds of reconstruction structures. (3) These findings confirmed that after BioFlex fixation, with the expansion of the range of decompression, the stress of adjacent segments of intervertebral disc gradual y increased, but different ranges of decompression cannot affect the stiffness of reconstruction structure.

Objective To investigate the efficacy of Yiqing capsules and Cefaclortablets in the treatment of simplex anaphylactoid purpura. Methods In this randomized,simple-blind, controlled study, all of 191 simplex anaphylactoid purpura patients,the throat secretion Group A βhemolytic streptococci antigen test was positive ,92 patients of control group were treated Cefaclor tablets and regular treatment,99 patients of test group were Yiqing capsules and Cefaclor tablets. Results After 10 days treatment,the clinical efficacy of the test group and the control group were 91.9% ,75.0% ,the test group was obviously higher than that of the control group(P ＜ 0.01), and the negative rate of the throat secretion Group A βhemolytic Streptococci antigen test of the test group was higher than that of control group, (P ＜0.01). the commencements of response of the test group was earlier than that of the control group(P ＜0. 01) ,the frequencies of recurrence of the test group were lower than that of the control group (P ＜ 0. 05).Conclusion Yiqing capsules combined cefaclor in treatment of simplex allergic purpura could improve efficacy, and reduce the treatment time.

Objective To investigate whether clinical,endocrine,and sonographic characteristics of infertile women with polycystic ovary syndrome (PCOS) could predict the ovarian response to clomiphene citrate (CC) medication.Methods A prospective study was conducted,85 women with PCOS were given CC 50 mg per day for 5 days.In the case of an absent response,doses were increased to 100 daily in subsequent cycles.Clinical,endocrine,and sonographic characteristics were compared between responders and nonresponders.Results The cycle history (oligomenorrhea or amenorrhea) and antral follicle count (AFC) were significantly different in responders from those in nonresponders (P ＜ 0.05).There were no differences in age,serum testosterone,follicle stimulating hormone (FSH),luteotropic hormone (LH),body mass index (BMI),insulin resistant index (HOMA-IR),and glucose and insulin levels between the two groups (P ＞ 0.05).In addition,multivariate logistic regression analysis indicated that cycle history and AFC were the predictors of ovarian response to CC treatment.Conclusions The cycle history and AFC can be used as the simple and effective parameters to predict ovarian response to CC treatment in PCOS patients.

Chitosan(CS)surface was modified with hydrophobic octyl groups to prepare N-octyl chitosan(nitro-gen-octyl chitosan;OC).Then hydrophilic group carboxyl-polyethylene glycol-amino (PEG);tumor-targeting lig-and D-glucosamine(DG);and membrane-penetrating peptide 9-D-arginine(9R)were linked to OC successively. Then the DG and 9R modified chitosan micelle (DG/9R-PEG-OC)with tumor-targeting and transmembrane effect was prepared.By hydrogen nuclear magnetic resonance spectrometer (1 H NMR)and sodium dodecyl sul-fate polyacryl amide gel electrophoresis(SDS-PAGE);the successful formation of DG/9R-PEG-OC was certified;with particle size of 151.8 nm and Zeta potential of 16.5 mV.The morphology of chitosan micelle observed by transmission electron microscope was homogeneous spherical structure.The drug loading content (DLC)(using fluorescein as a model drug)and encapsulation efficiency (EE)were about 28.2% and 75.0% measured by UV-visible spectrophotometer.Meanwhile;the drug showed a controlled releasing profile out of the micelle.Cellu-lar uptake experiments indicated DG/9 R-PEG-OC micelle had a significant tumor-tageting and transmembrane effects;especially on HepG2 cells;which exbihited high expression of the glucose transporter.Thus DG/9R-PEG-OC micelle could be a promising drug targeted delivery system of hydrophobic antitumor drugs.

OBJECTIVE: To standardize the management on drug use in primary hospitals in Beijing Dongcheng district so as to enhance the efficiency of drug administration.METHODS: A "Drug Use Monitoring Information Platform in Primary Hospital" comprising management information system on drug use,management system on information of drug wholesalers,drug monitoring management information system was established and its problems in the try-out were analyzed.RESULTS & CONCLUSIONS: The "Drug Use Monitoring Information Platform in Primary Hospital" still faced with the problems of legal basis,psychological concern,and construction of standard basic drug information database etc.However,its operation standardized the drug purchasing channel and management on drug use in hospitals of Beijing Dongcheng district and enhanced the drug administration level and efficiency,which thus deserves to be popularized throughout the country.

A methodology for preparing and certifying the reference material of 3-amino-2-oxazolidinone(AOZ) in eel muscle lyophilisates was presented. Furazolidone was accessed to eel by dipping fish in pond with furazolidone solution at a dosage of ca 0.16 mg/L. With the metabolism of furazolidone in eel, the muscles contain a certain concentration of AOZ as furazolidone metabolite was obtained. Lyophilization of the muscles was performed in one batch and 400 bags of samples were obtained by the procedure of homogenation, cryodesiccation and irradiation. The homogeneity and stability of the sample was examined. The value of the chemical constituent of the sample was certified through the collaborative analysis program participated by 11 laboratories using isotope dilution liquid chromatography-tandem mass spectrometry, and the uncertainty assessment was performed. The reference materials have been approved as certified reference materials by AQSIQ, China (State General Administration of the People′s Republic of China for Quality Supervision and Inspection and Quarantine) in 2009 after one year of trial period. The serial numbers is GBW(E)100180.

IgG4-related disease (IgG4-RD) has been proved to be associated with malignancy.The incidence and risk factors of malignancy development in IgG4-RD were not clear.Nine IgG4-RD patients with malignancies and 27 IgG4-RD control cases were analyzed for risk factors and clinical features.The incidence of malignancy in IgG4-RD was 3.3％,higher than age-controlled general population.Smoking history was significantly more common in patients with malignancies than in the control group (9/9 vs 16/27,P ＜ 0.05).A total of 6/9 malignancies occurred within the first year after the diagnosis of IgG4-RD.Colorectal,biliary and thyroid cancers were the leading types.Smoking history is a risk factor for IgG4-RD associated malignancy.Careful vigilance to monitor malignancy needs to be paid during follow-up.