Objective:To study the optimum ethanol extraction process. Methods: The extraction rate of extractum and total flavonoids as well scutellarin was choosen as the assessment index. The optimum ethanol extraction process was selected with the orthogonal design. Results: The optimum ethanol extraction process conditions follow as: adding 10 fold of 75% ethanol, extracting for 2.0h and twice in all. Conclusion: The average extraction rate of extractum and total flavonoids as well scutellarin was 13.6%, 91.7%, 78.4%(n=3), respectively.

ObjectiveTo investigate clinical symptoms and influencing factors of food intolerance and to identify susceptibility of food intolerance among adults with various Traditional Chinese Medicine (TCM) constitutions.MethodsA total of 411 individuals were recruited by using simple sampling method.ELISA was used to test serum levels of food intolerance specific IgG antibody.TCM constitution,food intake habit,lifestyle,and biochemical data were collected.Results ( 1 ) The prevalence of food intolerace was 60.1％ (247/411).(2) The most commonly seen food intolerance specific IgG were those against egg (44.9％ ),crab ( 37.7％ ),or cod ( 23.1％ ).Intolerance to 1 to 5 kinds of food could be found in single individual.Subhealthy people were more likely to suffer from food intolerance ( P＜0.05 ).( 3 ) Those with food intolerance always presented with various symptoms,mainly respiratory system symptoms.(4) The most common types of TCM constitution of the participants were gentleness constitution,qi-insufficiency constitution,yang-deficiencyconstitution,anddampness-heatconstitution.Thoseofnon-bloodstasis constitution were more likely to suffer from crab intolerance ( P＜0.05 ).Conclusion The prevalence of food intolerance is relatively high in Fuzhou areas.Subhealthy status,obesity women,and pathological constitutions may be risk factors of food intolerance.

Objective To investigate serum lipid profiles in newly diagnosed patients with polycystic ovary syndrome (PCOS) using lipidomics and correlate these features with hyperinsulinemia and hyperandrogenism associated with PCOS and obesity. Methods 32 newly-diagnosed PCOS women and 34 controls were enrolled and divided into obese and lean subgroups according to the body mass index (BMI). Anthropometric, biochemical, and hormonal parameters were collected. Serum lipid profiles including phospholipids, free fatty acids (FFAs), and bioactive lipids were analyzed using GC-MS and LC-MS. Results PCOS patients, in particular, the obese ones with fatty liver, have abnormal phosphatidylcholine (PC)/lysophospholipid (LPC) metabolism. PC was increased (16∶0, 18∶0, 18∶1, 18∶2, and 20∶4), while LPC was decreased (16∶0, 18∶0, and 18∶1; all P<0.05). Serum polyunsaturated fatty acids (PUFAs), were decreased significantly, and the long chain saturated fatty acid was increased. We also found that insulin stimulated the metabolism of PUFAs, but the androgen inhibits the metabolism of PUFAs by measuring their metabolites. Conclusion PCOS patients have metabolic disorders of phospholipids and PUFAs. Insulin stimulated while androgen inhibited PUFAs metabolism.