Objective To make clear the relationship between adenoma,malignant transformation of ade- noma and carcinoma in gallbladder.Methodes The nuclear morphometry was performed with a TJTY-300 Auto- matic Image Analyser and PCNA was measured with immunohistochemisty in 25 cases of carcinoma,20 cases of adenoma and 3 cases of normal gallbladder.Results (1) Cell DNA content,atypia and PCNA index were in- creased gradually from the normal cell to dysplasia to malignant transformation of adenoma to carcinoma in gallblad- der;(2) Aneuploidy in gallbladder adenoma and PCNA index had significant relationship to the size of adenoma and gallstones (P

Objective To estimative the relationship between ras gene mutation,PCNA overexpression and the recurrence and prognosis of gallbladder cancer.Methods PCNA was studied by ABC immunohistology, ras gene mutation by PCR-RELP analysis.Results Histology,Nevin staging and operative methods were relevant to the prognosis of gallbladder cancer.The patient with stag IV,V gallbladder cancer had a poor prognosis;radical operations could prolong patients survival time.The mortal risk of patients with ras gene mutation was 1.62 times higher than that without ras gene mutation,with high overexpression of PCNA was 2.2 times higher than that with low expression of PCNA. Conclusions Differentiation degree, Nevin′s staging, mutation of ras gene and overexpresion of PCNA could be the prognostic factors in gallbladder cancer,and could help to select the operative procedure and to eluvate the surrival.

Objective To explore the influnce and related factors analysis postpartum community visit for 6 months old infants exclusive breastfeeding (EBF).Methods 800 women in Daqing city communities delivered in January,2009 to December,2010 were selected into study based on simple random sampling.Using the questionnaire surveyed the influence of the postpartum community visit for 6 months old infants EBF.Results Reclaimed effective questionnaires were 790 ;6 months old infants EBF rate was 65.06％ ;the receiving postpartum community visit group's 6 months of EBF rate 7 4.8 7 ％) was significantly higher than the not receiving postpartum community visit group (38.50％),difference was statistically significant(x2 =90.54,P ＜ 0.01).Logistic regression analysis showed that main related factors of 6 months old infants EBF were the first breast feeding time,whether or not to accept the postpartum visit,mode of delivery and mother's education degree,respectively.Conclusion Postpartum community visit can promote 6 months old infants for EBF,but still need to pay more attention to coordination and improvement.

Objective To study the antioxidant activity of the extract from north Ph.praecox leaves.Methods The extract from north Ph.Praecox leaves was made by ultrasonic-assisted extraction process,and the concentration of the total flavonoids was detected by NaNO3-Al(NO3)3-NaOH methods.The antioxidant capacity of the extraction was detected by DPPH free radical scavenging method and CuSO4-Phen-Vc-H2O2-DNA reaction with ultra-weak chemiluminescence method.Results The bamboo leaf extract could scavenges the DPPH free radical with the IC50 value as 7.02 mg /L and could delay the development of the emission peak value(P

Objective To study and analyse the parameter values of voice in normal healthy preschool children and school-age children. Methods In present paper, voice quality of 120 cases were collected and analyzed with Dr. Speech software. Results There were no differences between preschool and school-age children on parameters jitter, shimmer, SNR, HNR, NNE, F 0, F 1,F 2,F 3 of voice acoustic and so in difference gender. Conclusion All parameters in preschool children and school-age children showed no difference. The tested parameters were significantly different between normal children and children with vocal nodule.

Objective To investigate the clinical and laboratory characteristics of anticentromere antibody (ACA)and anti-SSA antibody expressions in patients with Primary Sj(o)gren's Syndrome (PSS). Methods Twelve PSS patients with ACA positive but SSA negative(ACA PSS)and 19 PSS patients with SSA positive but ACA negative(SSA PSS)were enrolled into the study and classified into two groups. We compared the age,laboratory data,occurrence of Raynaud's phenomenon(RP),and histological changes in minor labial salivary glands biopsies of the patients from two group. Results The mean age of the ACA PSS group(68.4 ± 7.9)years was significantly higher than that of the SSA PSS(54. 6 ± 16. 2)years group(P ＜ 0. 05). Serum IgG level of ACA PSS group(17. 89 ±4. 08)g/L was close to the normal range,which was significantly lower than that of SSA PSS(27.90 ±6. 72)(P ＜0. 01). Leukocytopenia was less frequently observed in ACA PSS than in SSA PSS(P ＜ 0. 05),the difference between two groups was statistically significant. We also found more frequent RP in the ACA PSS group than SSA PSS group(P ＜ 0. 05). Conclusions Our data confirm that ACA positive PSS differs from SSA positive PSS at several clinical respects and laboratorial examinations.

Objective To investigate the changes in the abilities of invasion and metastasis in surviving human lung adenocarcinoma (A549) cells after X-ray irradiation and the related mechanism.Methods The change in radiosensitivity after X-ray irradiation was determined by colony formation assay.The abilities of invasion and metastasis were evaluated by MTF adhesion assay and Transwell invasion and migration assay in vitro.The mRNA and protein expression of E-cadherin,vimentin,tumor growth factor (TGF)-β1,matrix metalloproteinase (MMP)-2,and MMP-9 was measured by real-time RT-PCR using SYBR Green fluorescence and Western blot.Results The colony formation assay showed that the surviving A549 cells after X-ray irradiation were more resistant to irradiation (ratio of D0 values =0.94).Their abilities of adhesion,invasion,and migration were significantly increased by 1.46 times,1.40 times,and 1.45 times,respectively.In addition,the surviving cells showed enlarged intercellular spaces and had many long pseudopodi.Compared with those in the control group,the mRNA expression levels of vimentin,TGF-β1,MMP-2,and MMP-9 of surviving cells were increased by 1.37 times,2.37 times,1.80 times,and 1.50 times,respectively,the protein expression levels of the above substances were increased by 1.60 times,1.80 times,1.10 times,and 1.20 times,respectively,and the mRNA and protein expression levels of E-cadherin were decreased by 59.4％ and 74.6％.Conclusions The surviving A549 cells after X-ray irradiation have significantly increased abilities of invasion and metastasis.This may be due to radiationinduced TGF-β1 expression increase that in turn promotes epithelial-mesenchymal transition and also due to radiation-induced MMP-2 and MMP-9 expression increase.

Objective_To explore the influence and mechanism of IL-31 on the expression of VEGF, EGF and EG-FR in 16HBE cells.Methods_16HBE cells were cultured and treated with IL-31 with or without SB203580 or SP600125, real-time PCR and Western blot were applied to determine the mRNA and protein expression of VEGF, EGF and EGFR respectively.Meanwhile, Western blot was used to examine the changes of P38 MAPK and JNK signaling pathways.Results_Compared with control group, the mRNA expression of VEGF, EGF and EGFR was increased markedly under the stimulation of IL-31 ( P<0.01 ) , the expression of p-P38 MAPK and p-JNK signifi-cantly increased ( P<0.01) .Compared with IL-31 group, the expression of p-P38 MAPK significantly decreased in IL-31 combined with SB203580 or SB203580 group ( P <0.01 ) , while the expression of p-JNK markedly decreased in IL-31 combined with SP600125 or SP600125 group( P<0.01) .Compared with IL-31 group, the expression of VEGF was significantly decreased in IL-31 combined with SB203580 or SP600125 group ( P <0.01 ) , while the expression of EGF and EGFR was markedly declined in IL-31 combined with SB203580 group ( P<0.01 ) .Conclusions_IL-31 may up-regulate the expression of VEGF through activating P38 MAPK and JNK signaling pathways and up-regulate the expression of EGF and EGFR through activating P38 MAPK signaling path-way in16 HBE cells.

[Objective] To investigate the effect of aromatic herbs for resuscitation such as Moschus and Borneol on nitric oxide (NO) content and expression of endothelial NO synthetase (eNOS) and inducible NO synthetase (iNOS) in brain tissue of rats with cerebral ischemia and reperfusion injury. [Methods] Forty rats were allocated to 4 groups: mimic operation group (Group A), model group (Group B), herbs treatment group (Group C) and nimodipine treatment group (Group D). Rat model of cerebral ischemia and reperfusion injury was established by Pulsinelli 4VO method. NO content in brain tissue was determined by nitrate reductase method. Expression of eNOS and iNOS in micrangium and iNOS in neurons was measured by semi-quantitative analysis. [Results] NO content was decreased and approached normal level in Group C and Group D (P 0.05 as compared with Group A). Aromatic herbs with action of inducing resuscitation increased the expression of eNOS in micrangium and decreased the expression of iNOS in micrangium and neurons, the differences being significant as compared with those in Group B ( P

To investigate the effect of treatment based on syndrome differentiation combined with resuscitation inducing aromatic herbs for acute ischemic stroke (AIS).Forty four cases of AIS were randomly allocated to two groups. Twenty cases of Group A were treated with treatment based on syndrome differentiation combined with Tongqiao capsule(each capsule containing Moschus 0 04?g and Borneolum 0 1?g)and twenty four of Group B with treatment based on syndrome differentiation. Therapeutic effect were observed in the second week after medication. The total effective rate was 95% in Group A and 91 6% in Group B, the difference being not significant. However, the difference of markedly effective rate (80 0% in Group A and 54 2% in Group B) was significant (P