[Objective] Ursolic acid and oleanolic acid in Acantopanax senticosus (Rupr. et Maxim.) Harms were extracted with the ultrasonic technology. A determination method of ursolic acid and oleanolic acid was established with the high performance liquid chromatograph and photodiode array detector. [Method]The chromatographic column, Nova-Pak C18 (3.9?300 mm, 4 ?m), methanol-water (88∶12,V/V), mobile phase with 0.8 mL/min flow rate, the detected wave length (210nm),and the column temperature(25 ℃) were adopted. [Results] The calibration curve of ursolic acid was linear under the content of 0.136 ~1.224 ?g and the correlation coefficient was 0.9995. The calibration curve of oleanolic acid was linear under the content of 0.052 ~0.468 ?g and the correlation coefficient was 0.9994. The average recovery of ursolic acid was 98.1 % and RSD was 1.1 % (n=5). The average recovery of oleanolic acid was 97.5 % and RSD was 1.0 % (n=5). [Conclusion]The content determination of HPLC is accurate and reliable, and can be used to analyse quantificationally ursolic acid and oleanolic acid in Acantopanax senticosus (Rupr. et Maxim.) Harms.

OBJECTIVE:To further exploit the active components from Loquat leaf and its gruffs.METHODS:The chief components in loquat leaf and its gruffs were extracted by ultrasound extraction and Soxhlet extraction,respectively,the sepa?ration of ursolic acid and other constituents was performed by HPLC,and the relative amounts of which were determined and compared.RESULTS:Soxhlet extraction is superior to ultrasound extraction in the extraction of loquat leaf and8components in gruffs.Under the same extraction method,the content of ursolic acid in loquat leaves is equivalent to that of gruffs.Besides,the contents of6components in gruffs were above those in loquat leaves.CONCLUSION:The essential component-triterpenic acid in loquat leaves suffered no loss after extraction with water,the active components in gruffs can be further exploited.

AIM:To observe the contents of ursolic acid and oleanolic acid in Liuwei Dihuang Pill(Radix Rehmanniae praeparata,Rhizoma Dioscoreae,Fructus Corni,Rhizoma Alismatis,etc.) from different manufacturers and explore their effects on quality of Liuwei Dihuang Pill.METHODS:Ursolic acid and oleanolic acid in Liuwei Dihuang Pill were determined by HPLC-PAD method using Kromasil C_ 18 column(4.6 mm?250 mm,5 ?m) as chromatographic column,methanol-water-phosphate(88∶12∶0.02) as the mobile phase at the rate of 1.0 mL/min.RESULTS:6.462-0.718 ?g of ursolic acid and 3.132-0.348 ?g of oleanolic acid presented a good linear relationship.The average recoveres were 99.73% and 97.59%,RSD were 1.5% and 1.7%,respectively.There were significant differences in the contents of ursolic acid and oleanolic acid in Liuwei Dihuang Pill from four different manufacturers.CONCLUSION:The method is simple,rapid and feasible to set up determination of ursolic acid and oleanolic acid in Liuwei Dihuang Pill by HPLC-PAD.Although the contents of ursolic acid and oleanolic acid have significant differences in different manufactures,the results show good consistency in the content variation of the two acids.

Object To research and choose the best technology c onditions for extracting ursolic acid from Sambucus chinensis Li ndl. Methods According to physicochemical character of ursolic aci d, orthogonal design and tests of extract technology for ursolic acid were ca rried on, and four factors were chosen such as concentration, qu an tity of ethanol, extracting time, concentration of clearing agent, and three lev els of each factor were used for orthogonal design and test. Results That was the best technology condition that ursolic acid was extracted by means of ethyl alcohol (90%) of 7 times as much as raw materials, and it is heat ed 2 times (each 1 h), and the concentration of clearing agent was 3%. Conclusion That is a better technology for industrial producti on because it is advanced and rational, practical and feasible.