Object To research and choose the best technology c onditions for extracting ursolic acid from Sambucus chinensis Li ndl. Methods According to physicochemical character of ursolic aci d, orthogonal design and tests of extract technology for ursolic acid were ca rried on, and four factors were chosen such as concentration, qu an tity of ethanol, extracting time, concentration of clearing agent, and three lev els of each factor were used for orthogonal design and test. Results That was the best technology condition that ursolic acid was extracted by means of ethyl alcohol (90%) of 7 times as much as raw materials, and it is heat ed 2 times (each 1 h), and the concentration of clearing agent was 3%. Conclusion That is a better technology for industrial producti on because it is advanced and rational, practical and feasible.

Objective To explore the clinical efficacy and safety of umbilical cord derived mesenchymal stem cells transplantation(UC-MSCT)for patients with refractory systemic lupus erythematosus (SLE).Methods Twelve patients with refractory SLE were enrolled in this study.UC-MSCs(≥106/kg cell number)were infused intravenously for each patient. The clinical manifestations and laboratory parameters were compared before and after MSCT. Results The twelve patients were followed up for one to twenty-six months after MSCT.The systemic lupus erythematosus disease activity index(SLEDAI)score decreased from 18±4 to 10±4 one month after MSCT(n=12,P＜0.01)and then decreased to 7±4 at three month follow-up.Nine patients showed improvement of 24 h proteinuria[(2103±749)mg vs(3359±1248)mg,P＜0.01]one month after MSCT.Further improvement of 24 h proteinuria was observed in eight patients[(1427±616)mg vs(3342±1333)mg,P＜0.01]at three months post MSCT.Serum creatinine of five patients decreased significantly and ten patients showed an increase of serum albumin. Serum complement C3 increased in three patients and four patients showed obvious amelioration of hematological abnormalities. There was no transplantation related complications for all the patients. Conclusion UC-MSCT is effective and safe for refractory SLE,but further observation is required to evaluate its long term efficacy.