Objective To investigate the efficacy of MSCs infused intravenously on the regeneration of injured spinal cord and rehabilitation of its neurological function. Methods 32 SD rats, male or female, weight about 300 g for each one. MSCs were separated, cultured and purified in vitro. Surface marker of MSCs, such as CD34, CD45, CD29 and CD90 were detected by flow cytometry. The rat spinal cord injury model was prepared according to the modified Allen method. After exposure of T10 spinal cord, the T10 segment of spinal cord was injuried by a 10 g weight falling down from 5 cm high place upon a round thin copper pad which was placed on the surface of T10 segment of spinal cord. The diameter of the copper pad is 3 mm. There are 24 rats in the injuried group and 8 in the non-injuried group. The injuried group was then divided into experiment group with 14 rats and control group with 10 rats at 24 hours after preparation of models. The rats in the injuried group and non-injuried group were infused with MSCs marked by Brdu through tail vein, and the rats in control group were infused with PBS. The neurological functions of rats were evaluated at 24 hours after injury and 1, 3, 5 weeks post-infusion respectively. At the same time, the immigration, survival and differentiation of MSCs were observed. Results The MSCs were uniformly CD29, CD90 positive and CD34, CD45 negative. In vivo experiment, transplanted MSCs survived and were localized to the injured spinal cord, and a few cells expressed NSE, MAP2 post transplantation 3 to 5 weeks. Significant improvement in functional outcome in rats treated with MSCs transplantation compared to control rats. The score of BBB in the treated group was higher than that in the control group (P

Objective To investigate the way to accurately delineate gross tumor volume (GTV) of high grade gliomas(HGG) for intensity modulated radiation therapy (IMRT) by using computed tomography (CT) and magnetic resonance imaging (MRI) image fusion technique. Methods CT and MRI images were fused from 19 patients. The GTV of each patient were independently delineated by one chief doctor and one resident doctor on CT and MRI image. The GTV contoured on CT (GTVCT), MRI (GTVMRI) were measured, and composite volumes (GTVCT+MRI) were the sum of CT-defined GTV and MRI-defined GTV. The differences of these volumes were compared. Results Whether chief or resident doctors delineated, all were GTVMRI ＞GTVCT(P ＜0.050). The percentages of GTVMRI on GTVCT+MRI were (98.57±7.00)% by chief doctors, and (97.84±10.00)% by resident doctors. Compared the difference between GTVCT and GTVMRI in postoperative patients and preoperative patients, P =0.046, and the difference between chief doctors and resident doctors was statistically significant for GTV defined by CT (P =0.020), but not by MRI and composite image (P ＞0.050).Conclusion The GTV of HGG patients must be delineated on both CT image and MRI image, including using CT and MRI image fusion. But the composite volumes(GTVCT+MRI) should be the sum of CT-defined GTV and MRI-defined GTV. Especially for the postoperative patients,delineating GTV should be taken more attention. And the GTV should be delineated by doctors with full experiences.