【Objective】To investigate the effect of serum containing Yifa Compound(YC) on the growth of hair papilla cells.【Methods】Hair papilla cells were cultured in vitro.After subculture for 4～6 generations,hair papilla cells at logarithmic growth phase were cultured with YC-containing serum(at the concentrations of 1.25,2.5 and 5.0?g/L respectively) and blank serum respectively.After the culturing,the proliferation curve of hair papilla cells was drafted by methylthiazolyltetrazolium(MTT) assay and their growth was observed under scanning electron microscope.【Results】Optical density of hair papilla cells was higher at logarithmic growth phase while lower at growth lag phase in high-dose YC-containing serum group than that in blank serum group.Moderate-and high-dose YC-containing serum promoted the agglutinating radial growth.【Conclusion】Serum containing YC can promote the proliferation of hair papilla cells.

Recently diverse magnetic resonance imaging technology is used in glioma grading,which based on the theory of Gaussian distribution.Diffusion kurtosis imaging emerges as an extension of DWI and DTI,which could reflect the real and actual hydron non-Gaussion distribution of tumor and peritumoral microenvironment.Displaying the correlation with immunohistochemical label Ki-67,DKI convinces more scholars inland and abroad with its practicability.

Objective Study of the feasibility that the former changing trend of tumour after the first BAI can forecast the later curative effect on tumour.Methods Bronchus artery and relational body arteries opacification and BAI have been introduced in 82 primary pulmonary carcinoma cases.In the statistic analysis,SPSS 13.0 for Windows Stat.Software Package has been used.At the meanwhile chi square test,one-sample t-test,GLM-univariate analysis have been adopted as well to study the relationship between the former changing trend of tumour and Later Curative Effect after BAI in mid-advanced stage pulmonary carcinoma.Result There are significance statistical significances between the former changing trend of tumour and the later curative effect after the first BAI in mid-advanced stage pulmonary carcinoma.Conclusion The former changing trend of tumour after the first BAI is the factor influencing the later curative effect.

Objective To study later eurative effects after BAI with single factor analysis.MethodsBronchus artery and relational body arteries opacification and BAI had been introduced in 82 primary pulmonary carcinoma cases.In the statistic analysis,SPSS 13.0 had been used.ResultsThe tumour blood supply types and the times of effective BAI treatment were the factors influencing the later treatment period.Tumour pathologic types,the branches of tumour blood supply,tumour clinic cTNM stages,chems project influenced faintly later curative effect.ConclusionThe blood supply types of primary pulmonary carcinoma and the times of effective BAI treatment are the factors influencing the later treatment period.

Objective To identify the cell death type and investigate the potential mechanism of ionizing radiation-induced neural cell death in the neonatal rat cerebral cortex.Methods The neonatal Wistar rats were given a single dose of 2.0 Gy γ-irradiation.The cell death type and characterization in cerebral cortex were identified using DNA electrophoresis,TUNEL and HE staining.The P53-and iNOS-positive cells were analyzed quantitatively using immunohistochemistry.Results The DNA and morphological characterization of death cells indicated that 2.0 Gy γ-irradiation induced apoptosis of the neural cells in neonatal rat cerebral cortex.The apoptosis indices in different cortex regions were significantly increased 4 h after irradiation,and reached the peak value at 12 h post-irradiation.The apoptosis index of neoconex was much higher than that of hippocampus(archicortex)and paleocortex,while paleocortex had lower apoptosis index than hippocampus.The quantitative immunohistoehemistry suggested that the numbers of P53 and iNOS-positive cells were not different between these three cortex regions at the same time-point after irradiation.Conclusion 2.0 Gy γ-rays induced apoptosis of the neural cells in neonatal rat cerebral codex.The response of cells to the damage effects of ionizing radiation was similar in different cortex regions;however,the apoptosis indices were different significantly.These findings imply that the developing phase or type of neural cells may play a pivotal role in the apoptosis process induced by ionizing radiation.

Objective To explore the therapeutic effects of human glial cell line-derived neurotrophic factor (GDNF)-engineered rat neural stem cell (NSC) transplantation in rat model of Parkinson's disease ( PD) . Methods SD rats received a single injection of 24 μg of 6-hydroxydopamine (6-OHDA) at two sites in right striatum. Then 10 days after surgery, the successful animal models of PD were divided into 3 groups: PD model group ( 2 μl transplantation media was injected in right striatum), NSC group (transplanted were 2×10~5 NSCs infected by bare lentivirus) and GDNF group (transplanted were 2×10~5 GDNF-engineered NSCs). The rotation scores were assessed 5 weeks, 7 weeks and 9 weeks after transplantation. The dopaminergic neurons in substantia nigra ( SN ) were analyzed quantitatively using immunohistochemistry for tyrosine hydroxylase (TH), and the dopamine and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were analyzed 9 weeks after transplantation by high performance liquid chromatography ( HPLC) . Results GDNF-engineered NSC transplantation could effectively improve the behavioral performance in rats. At the 5th week after cell transplantation, the rotation turns within 90 min were (993. 9±159. 1) turns, (956. 7±136. 3) turns and (433. 6±100. 9) turns in PD model group, NSC group and GDNF group respectively (F=95. 694, P = 0. 000). At the 7th week, the rotation turns within 90 min were (964. 2 ± 152.0) turns, (909. 2 ± 136. 3) turns and (399. 4±84. 4) turns in PD model group, NSC group and GDNF group respectively (F = 106. 134, P=0. 000). At the 9th week, the rotation turns within 90 min were (909. 5±152. 2) turns, (865. 5± 129. 1) turns and (312. 2±63. 7) turns in PD model group, NSC group and GDNF group respectively (F= 151. 100, P = 0.000). GDNF-engineered NSC transplantation could significantly increase the levels of dopamine and its metabolites in injured striatum. The concentrations of dopamine in injured striatum was higher in GDNF group than that in PD model group and NSC group C(7. 5±0. 8) ng/mg vs. (3.3±0.3) ng/mg and (3. 7±1. 3) ng/mg, F=59. 543, P = 0. 0003. The level of DOPAC was higher in GDNF group than that in PD model group and NSC group C(0. 9±0. 1) ng/mg vs. (0. 5± 0. 1) ng/mg and (0. 6±0. 2) ng/mg, F= 17. 293, P=0. 000]. The concentration of HVA in injured striatum was higher in GDNF group than that in PD model group and NSC group [(0. 9±0. 1) ng/mg vs. (0.5±0. 1) ng/mg and (0. 6±0. 2) ng/mg, F=35.175, P = 0.000]. Conclusions engineered NSC transplantation improves the function of dopamine system in SN and striatum, and GDNF gene therapy has potential clinical value.

Objective A lot of drugs have side effects on the central nerves system. Especially in children. In vivo neurotoxicity tests are time-consuming and expensive. The neural differentiation of human embryonic stem cells and amniotic fluid stem cells provides all ideal in vitro system that Can be applied to evaluate neurotoxicity of drugs. This study was to try to establish such a system. The kainie acid was selected to test the neurotoxicity. Methods The human embryonic stem cells and amniotic fluid stem cells were indueed to differentiate into neural cells by a chemically defined neural induction medium. The induced neural cells were propagated in the presence of basic fibroblast growth factor. Immunocytochemical staining Was applied to confirm these cells' neural identity. The induced cells were propagated under different concentration of kainic acid, then the gosh curve were made based on the cell numbers. Results Both of the human embryonic stem cells and amniotic fluid stem cells could be efficiently induced to be differentiated into neural cells. The neural differentiation efficiency of human embryonic stem cells is higher than that of human amniotic fluid stem cells. The kainic acid has neurotoxieity to the indueed neural cells. Conclusions The neural differentiation of human embryonic stem cells and amniotic fluid stem cells were proved to provide a rapid and convenient approach for estimating the neurotoxlcity of drugs.

Objective To investigate the diagnostic values of color ultrasound to the stenosis of extracranial carotid arteries.Methods Color ultrasound examination and digital subtraction angiography(DSA)were performed in 145 patients with ischemic cerebravascular disease.The sensitivity and specificity of color ultrasound for the stenosis of extracranial carotid arteries were analyzed according to the golden standard of DSA results.Results The sensitivity and specificity of color ultrasound for stenosis of carotid bifurcation,subclavicular artery and vertebral artery were 96.8%,80.6%,60.0% and 98.7%,33.3%,89.0%.The accuracy of color ultrasound for stenosis of carotid bifurcation,subclavicular artery and vertebral artery were 93.0%,92.8% and 82.1%.Conclusion Color ultrasound examination are effective for the diagnosis of carotid bifurcation sterosis,and less effective for the diagnosis of the subclavicular and vertebral artery stenosis because of the poor sensitivity.

Objective To identify the clinical features of patients with brucellosis in Liaoning Province in recent 5 years, and to improve the diagnostic level of the disease.Methods The clinical data including epidemiology, clinical features, laboratory data and diagnosis of 82 hospitalized patients who were diagnosed with brucellosis in the First Affiliated Hospital of China Medical University from 2011 to 2015 were collected and reviewed retrospectively.Results The majority of the 82 patients were middle-aged (45-59 years old) (47.6%) and male gender (63.4%).Fever (90.2%), muscle and joint pain (61.0%), hepatosplenomegaly (36.6%), lymphadenopathy (24.4%) and weight lose (32.9%) were the main complaints.The peripheral leucocyte counts were usually normal (70.7%).C-reactive protein (81.3%), procalcitonin (81.8%) and erythrocyte sedimentation rate (64.2%) increased in most cases.The results of the serum agglutination test in 67 patients were all positive (100.0%).The pathogen isolation was conducted in 60 patients and 49 patients were positive for Brucella species.Among them, 35(71.3%) isolates were identified as Brucella melitensis.A total of 90.2%(74/82) cases were firstly diagnosed with fever of unknown origin and were hospitalized.Misdiagnosis accounted for 52.4(43/82)%.Conclusions Contact history with cow and sheep should be inquired thoroughly in patients with clinical manifestations of fever, muscle and joint pain and hepatosplenomegaly.Pathogen isolation, agglutination test and other specific tests should be performed as soon as possible to diagnose the disease early.

Objective To investigate the clinical effect of mesocaval shunt and selective coronary vein ligation for portal hypertension caused by cavernous transformation of the portal vein in adults.Methods The clinical data of 26 adult patients with portal hypertensive variceal bleeding caused by cavernous transformation of the portal vein treated by mesocaval shunt plus splenic artery and coronary vein ligation were analyzed retrospectively from 2001 to 2011.Results There was no operative deaths,free portal vein pressure decreased postoperatively,differences were significant before and after shunt (34.4 ± 4.7) cm H2O vs.(24.8 ±2.7) cm H2O,t =12.30,P ＜0.01.Postoperative complications included slight hepatic encephalopathy in 1 case,stress ulcer bleeding in 1 case,which were cured by conservative treatment.23 cases were followed up from 6 months to 4.5 years,and there was no bleeding case.Conclusious Mesocaval shunt plus selective vascular ligation for portal hypertensive variceal bleeding caused by cavernous transformation of the portal vein in adults is safe and effective.