Objective To investigate the function of HOXa-10 gene in the course of mouse blastocyst implantation. Methods Real-time fluorescence quantitative PCR was applied to detect the expression of HOXa-10 gene in normal mice and day1,day2,day3 day4,day5 and day7 pregnant mice.Plasmid containing the HOXa-10 cDNA fragment with liposome and plasmid containing HOXa-10 antisense oligonucleotides with liposome were transfected respectively into the uterine horns of day 2 pregnant mice,and the number of blastocyst-implanted was counted and compared with that of normal day 2 pregnant mouse. Results HOXa-10 gene expression in the pregnant group is higher than that of the non-pregnant group,and the results showed a gradual increasing trend as days went by: HOXa-10 gene expression was the highest on pregnancy d4,began descending on pregnancy d5 and was close to that of non-pregnancy on pregnancy d7.The number of blastocyst increased in the uterine horns which were injected with plasmid containing the HOXa-10 cDNA fragment with liposome.On the contrary,the number of blastocyst decreased in the uterine horns which were injected with plasmid containing HOXa-10 antisense oligonucleotides with liposome(P

Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) enables non-invasive imaging characterization of tissue vascularity with small molecular weight gadolinium chelates. Depending on this technique, tissue blood perfusion, microvessel permeability and extracellular leakage space can be obtained. The basic principles of two dynamic MRI techniques (T2*W and T1W DCE-MRI) and their applications in prostate cancer of DCE-MRI including diagnosis, differential diagnosis, formulation of treatment plan, evaluation of therapeutic reaction, detection of lesion recurrent were reviewed in this article.

To analyze the sex and STDs, premarital sexual status and attitudes of students of a medical university with survey data collected.Methods: 1850 questionnaires were issued and 1850 valid responsive sheets were collected. Results: Of all the respondents, 16.8% with premarital sexual behavior, 46.56% once took some contraceptive measures, 25.57% adopted outside ejaculation, 50.49% thought that masturbation was harmful, 15.68% of them couldn't answer some questions correctly about sex and reproductive health. The result shows that taking sex and reproductive health education for college students are necessary. Parents and schools should recognize and take the responsibility of sex education for the younger generation.

Objective: To explore the biological characteristics of synovial fluid-derived mesenchymal stem cells (SF-MSCs) cultured in serum-free medium and the ability of in vitro reconstruction of three-dimensional cartilage combined with scaffold material. Methods: Human SF-MSCs were cultured in serum medium and mesenchymal stem cells medium-serum free (MSCM-sf) respectively, then the proliferative ability and morphology of SF-MSCs were compared; The third passage SF-MSCs cultured in MSCM-sf were identified by flow cytometry, three-way(chondrogenic, osteogenic, adipogenic)differentiation assay and induced for chondrogenic differentiation when combined with polyglycolic acid/polylactic acid (PGA/PLA). Results: SF-MSCs cultured in MSCM-sf had better morphology and proliferative ability than that cultured in serum medium. The expression levels of positive markers of the third passage SF-MSCs cultured in MSCM-sf, such as CD73 (99.5%), CD90 (98.9%) and CD105 (96.5%), were more than 95%. However, the overall negative markers (CD34, HLA-DR and CD11b) expressed less than 2%. Three-way differentiation staining was positive. The combination of SF-MSCs and PGA / PLA can be induced into cartilage in vitro. Conclusions SF-MSCs cultured in MSCM-sf can be amplified under the condition of maintaining the stem cell characteristics, and can be combined with PGA/PLA scaffold to construct three-dimensional cartilage in vitro.

Objective To develop a new method to determine the contents of rutaecarpine in Fuzhengpingxiao capsule by HPLC method.Methods Samples were handled by ethanol and extraction with ethyl acetate.The separation was achieved on an Agilent TC-C18column using a mobile phase system of acetonitrile-water(2% Tetrahydrofuran and 0.2 % formic acid)at a flow rate of 1.0 ml/min.The temperature of column was 40 ℃ and the detection wavelength was 240 nm.Results The cali-bration curves of rutaecarpine showed good linearity in the ranges of 1.18-118 μg/ml,r=0.999 9.The results of intra-day and inter-day precisions were both within 2%,the average additional recovery rate was 94.20%.Conclusion The HPLC method was accurate,specific,sensitive and reproducible,which could be used for quality control of rutaecarpine in the preparation of Fuzhengpingxiao capsule.

Objective To establish a UPLC-MS/MS method for the determination of dexmedetomidine in human plasma and investigate the effect of obstructive jaundice on pharmacokinetics of dexmedetomidine in vivo. Methods Samples were obtained by liquid-liquid extraction. Agilent Eclipse Plus C₁₈ column was used for chromatograph with methanol and 0.1% formic acid-water solution as mobile phase. Flow rate was 0.2 ml/min. The column temperature was 35 ℃, and the MS detection was selected in MRM mode. Results The calibration curves of dexmedetomidine showed good linearity in the ranges of 0.01−10.00 ng/ml. The results of intra and inter-day precisions were both within 15%. The recovery rate was 85.5%−93.1%. Matrix effect was 91.2%−95.6%. Samples remained stable during analysis. Compared with the control group, c_max、AUC_(0−t)、AUC_(0−∞) and V_z of dexmedetomidine in the patients with obstructive jaundice were increased by 63.4%, 78.9, 66.4%, 82.5%, respectively (P<0.01). CLz was decreased by 42.1%. Conclusion This method is accurate, sensitive and reproducible. It is suitable for dexmedetomidine assay in human plasma. The elimination rate of dexmedetomidine is slower in obstructive jaundice.