Objective To analyze the relationship between the concentration of plasma insulin during oral glucose tolerance test (OGTT) and stent restenosis in non-diabetic patients, and determine whether hyperinsulinemia during OGTT is a predictor of the development of stent restenosis. Methods 49 non-diabetic patients with coronary artery disease underwent stent implantation were enrolled in this study. All patients were subjected to 75g OGTT before or after stent implantation, and coronary angiography was performed to observe the stent restenosis about 6 months of post-operation. Results The concentrations of plasma insulin at 0 5, 1, 2 and 3 hours after glucose loading were significantly higher in patients with stent restenosis than those in patients without stent restenosis(P≤0 05). Insulin area(IA) and insulin area/glucose area(IA/GA) were also significantly higher in patients with stent restenosis than those in patients without stent restenosis(P≤0 05). Conclusion Non-diabetic patients with hyperinsulinemia during OGTT had a higher risk for stent restenosis after stent implantation, and OGTT before stent implantation may be useful to predict stent restenosis.

Objective To investigate the protection of metformin on kidney in non-diabetic hypertension elderly.Methods The patients was divided into two groups;one given nifedipine ,another given nifedipine plus metformin. Three months Later,the changes of body mass index (BMI), plasma insulin, urine protein, blood urea nitrogen(BUN), blood and urine ? 2-microglobulin(? 2-MG) were examined. Results Above-markers were reduced more remarkably in nifedipine plus metformin group than that in only nifedipine group(P

Objective To investigate the clinical significance of vascular endothelial growth factor (VEGF) levels in the serum of patients with acute myocardial infarction (AMI),and also to explore the relationship between VEGF and creatine kinase (CK) levels in AMI.Methods 25 patients with AMI and 12 controls were used for this study. Serum was isolated from peripheral blood on day 1, 5, 10 and15 after the onset of AMI. VEGF levels were measured by enzyme-linked immunosorbant assay (ELISA).Results Serum VEGF levels elevated gradually after the onset of AMI and reached a peak on day 10, which was significantly higher than that of control group (178 9?48 9)pg/ml vs(64 9?16 7)pg/ml,P

Objective To observe the dynamic change of the intimal proliferation in balloon-injured rabbit carotid arteries to explore the mechanism of restenosis. Methods 40 healthy male New Zealand white rabbits were randomly divided into experiment groupsⅠ、Ⅱ、Ⅲ and control group, each group containing 10 rabbits. Right carotid artery was injured with PTCA balloon in the experiment groups. 10 rabbits in each experiment group were killed on 1, 2 and 4 weeks after injury. Morphological change of injured intimae was examined by light microscope and analyzed using a computerized imaging analysis system. Results In experiment group, neointimal areas were significantly more than those in control group(P

Objective To investigate IL-18 expression of monocyte cells and the inhibition effect of fluvastation on its expression in patients with acute coronary syndrome(ACS). Methods Isolated monocyte cells from peripheral blood of patients with ACS were incubated with different concentrations of fluvastatin, and IL-18 mRNA level of the monocyte cells was detected by reverse transcription polymerase chain reaction(RT-PCR). Results The monocyte cells of peripheral blood in patients with ACS expressed IL-18 mRNA. IL-18 mRNA decreased from 54 1?9 9 to 50 1?12 6(P

Objective The aim of this study was to examine plasma levels of CD40 ligands (CD40L) and pregnancy-associated plasma protein-A in patients with angiography type Ⅱ lesions (representing plaque rupture with or without thrombosis), and to evaluate the role of PAPP-A in the development of plaque disruption. Methods The morphology of the plaques in 68 patients was studied and analyed by means of coronary angiography. According to the morphology of the plaques, the patients were divided into type Ⅰ group (smooth borders, n=19), type Ⅱ group (irregular lesions, n=33), and type Ⅲ group (long lesions, n=16). The plasma level of CD40L, PAPP-A, creatine kinase (CK), and MB isoenzyme of CK (CK-MB) was measured in all the patients. Results Mean plasma CD40L [(3.21?2.08)mg/L] in type Ⅱ group was significantly higher than that in the type Ⅰ group [(1.03?0.98)mg/L, P

Objective To study the effect of G-CSF and SCF on ventricular remodeling after acute myocardial infarction in rats. Methods Left anterior descending coronary artery ligation was performed on 78 rats and after 6 hours there were 60 rats survived. The 60 rats were randomly divided into 2 groups: 30 rats received the treatment of G-CSF and SCF while the other 30 rats served as the control. The treatment group was injected subcutaneously with G-CSF and SCF and the control group was given saline. The treatment and control groups were randomly divided into 3 subsections and were killed in the 1~(st), 2~(nd) and 4~(th) week respectively. Comparison between the two groups was made in terms of ratio of ventricular weight to body weight, percentage of the infarction area to the total surface area of the LV as well as HE and immunohistological staining of the myocardium. Results (1) In the 4~(th) week, the ratio of ventricular weight to body weight in the treatment group was smaller (P

BACKGROUND:Regulation of neural stem cells differentiation hampers its application,and the regulation mechanism remains poorly understood.Anterior subventricular zone(SVZa)is one of the rich zones of neural stem cells.The neural stem cells at SVZa can immigrate for a long distance with undifferentiated states and finally differentiate into intemeurons at the olfactory bulbs.Bone morphogenetic protein-2(BMP2)and DLX5 play a significant role in immigration and differentiation of neural stem cells at SVZa towards olfactory bulb.OBJECTIVE:To study the feature of construction and immigration of neural stem cells at SVZa,and to review the role of BMP2 and DLX5 in neural stem cells differentiation,in addition,to explore the regulation mechanisms of neural stern cells differentiation.METHODS:Literatures from PubMed database(http://www.ncbi.nlm.nih.gov/PubMed),CNKI database(www.cnki.net/index.htm)and WANFANG database(http://www.wanfangdata.com.cn)published between January 1997 and January 2009 were searched with the key words of"neural stem cells,SVZa,BMP2,and DLX5".The repetitive and obsolete studies were excluded.RESULTS AND CONCLUSION:A total of 121 literatures were selected and primarily collected,including 86 Chinese literatures and 35 English literatures.Finally,according to standardization,28 literatures were further analyzed.Neural stem cells of SVZa have unique construction and can immigrate for a long distance.BMP2 and DLX5 play a significant role in neuronal immigration and differentiation of the NSCs of SVZa,but the detailed mechanism of BMP2 and DLX5 in SVZa is not clear.

BACKGROUND:At present,there are two main methods of isolating and purifying bone marrow mesenchymal stem cells(BMSCs):density gradient centrifugation and the whole bone marrow adherent method.The former has complicated procedures,and the latter has simple operation,but the purified outcomes are not ideal.OBJECTIVE:To establish a rat BMSCs isolated and cultured in vitro purification methods on the basis of the whole bone marrowadherence and isolation of BMSCs in combination of differential passage digestion method.METHODS:By whole bone marrow adherent culturing to isolate and differential digestion and passage of rat BMSCs,the speed ofMSCs in the process of digestion and passage was quicker than other bone marrow cells,as well as the characteristics of adherentspeed,instead of density gradient centrifugation to separate and purify MSCs,and their morphological characteristics wereobserved.Cell growth and proliferation of two kinds of culture method were compared with the density gradient centdfugationseparation.Alkaline phosphatase and oil red staining results were observed to verify BMSC differentiation capacity,to detect thecall surface markers,to validate immune properties and to test its purity.RESULTS AND CONCLUSION:The whole bone marrow adherent culture method isolated and differentially subcultured ratBMSCs.Flow cytometry and osteogenic and adipogenic culture results displayed cytoirnmunity property,purity and differentialcapacity,which have no significant difference compared with density gradient centrifugation.However,cell viability andreproductive activity are obviously elevated.

Objective To observe the effect of anxiety and depression on cell adhesion molecules and fibrolytic activity in cardiac-pacing patients. Methods The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 were measured in 39 cardiac-pacing patients with anxiety or depression as rersearch group, 32 cases cardiac-pacing patients without any psychological problems as observation control group and 20 healthy subjects as normal control group. Result The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 in cardiac-pacing patients were significantly higher than those in normal control group, and their plasma levels in the patients with anxiety or depression were significantly higher than those in the patients without psychological problems. Conclusion There were significantly elevated cell adhesion molecules level and decreased fibrolytic activity in cardiac-pacing patients. The anxety and depression status could aggregate this condition.