Objective To analyze the relationship between the concentration of plasma insulin during oral glucose tolerance test (OGTT) and stent restenosis in non-diabetic patients, and determine whether hyperinsulinemia during OGTT is a predictor of the development of stent restenosis. Methods 49 non-diabetic patients with coronary artery disease underwent stent implantation were enrolled in this study. All patients were subjected to 75g OGTT before or after stent implantation, and coronary angiography was performed to observe the stent restenosis about 6 months of post-operation. Results The concentrations of plasma insulin at 0 5, 1, 2 and 3 hours after glucose loading were significantly higher in patients with stent restenosis than those in patients without stent restenosis(P≤0 05). Insulin area(IA) and insulin area/glucose area(IA/GA) were also significantly higher in patients with stent restenosis than those in patients without stent restenosis(P≤0 05). Conclusion Non-diabetic patients with hyperinsulinemia during OGTT had a higher risk for stent restenosis after stent implantation, and OGTT before stent implantation may be useful to predict stent restenosis.

BACKGROUND:Regulation of neural stem cells differentiation hampers its application,and the regulation mechanism remains poorly understood.Anterior subventricular zone(SVZa)is one of the rich zones of neural stem cells.The neural stem cells at SVZa can immigrate for a long distance with undifferentiated states and finally differentiate into intemeurons at the olfactory bulbs.Bone morphogenetic protein-2(BMP2)and DLX5 play a significant role in immigration and differentiation of neural stem cells at SVZa towards olfactory bulb.OBJECTIVE:To study the feature of construction and immigration of neural stem cells at SVZa,and to review the role of BMP2 and DLX5 in neural stem cells differentiation,in addition,to explore the regulation mechanisms of neural stern cells differentiation.METHODS:Literatures from PubMed database(http://www.ncbi.nlm.nih.gov/PubMed),CNKI database(www.cnki.net/index.htm)and WANFANG database(http://www.wanfangdata.com.cn)published between January 1997 and January 2009 were searched with the key words of"neural stem cells,SVZa,BMP2,and DLX5".The repetitive and obsolete studies were excluded.RESULTS AND CONCLUSION:A total of 121 literatures were selected and primarily collected,including 86 Chinese literatures and 35 English literatures.Finally,according to standardization,28 literatures were further analyzed.Neural stem cells of SVZa have unique construction and can immigrate for a long distance.BMP2 and DLX5 play a significant role in neuronal immigration and differentiation of the NSCs of SVZa,but the detailed mechanism of BMP2 and DLX5 in SVZa is not clear.

BACKGROUND:At present,there are two main methods of isolating and purifying bone marrow mesenchymal stem cells(BMSCs):density gradient centrifugation and the whole bone marrow adherent method.The former has complicated procedures,and the latter has simple operation,but the purified outcomes are not ideal.OBJECTIVE:To establish a rat BMSCs isolated and cultured in vitro purification methods on the basis of the whole bone marrowadherence and isolation of BMSCs in combination of differential passage digestion method.METHODS:By whole bone marrow adherent culturing to isolate and differential digestion and passage of rat BMSCs,the speed ofMSCs in the process of digestion and passage was quicker than other bone marrow cells,as well as the characteristics of adherentspeed,instead of density gradient centrifugation to separate and purify MSCs,and their morphological characteristics wereobserved.Cell growth and proliferation of two kinds of culture method were compared with the density gradient centdfugationseparation.Alkaline phosphatase and oil red staining results were observed to verify BMSC differentiation capacity,to detect thecall surface markers,to validate immune properties and to test its purity.RESULTS AND CONCLUSION:The whole bone marrow adherent culture method isolated and differentially subcultured ratBMSCs.Flow cytometry and osteogenic and adipogenic culture results displayed cytoirnmunity property,purity and differentialcapacity,which have no significant difference compared with density gradient centrifugation.However,cell viability andreproductive activity are obviously elevated.

Objective Interferon regulatory factors 3 (IRF-3) is a key transcription factor to regulate gene expression of interferon after virus infection. This study aims to look for new spliced isoforms of IRF-3 and to investigate their structures and functions. Methods RNA extracts from human embryonic kidney 293 cells were amplified by RACE and RT-PCR. New sequences were compared with published sequences of IRF-3 and murine EST database using bioinformatics method. A new sequence, IRF-3c, was subcloned into pcDNA3.1-flag. The IRF-3c/pcDNA3.1-flag plasmid was transfected in HEK 293 cells. Whole cell extract was analysed by Western blot and then probed with monoclonal Flag antibody. Luciferase assay was carried out by co-transfection with reporter plasmid, pGL2B with interferon ? promoter, and IRF-3c cDNA expression plasmid. At 16 hours posttransfection, cells were infected with Sendai virus for 8 hours. Cells were collected and assayed for luciferase activity. Results A novel spliced isoform of IRF-3, named IRF-3c was discovered. The new isoform is almost the same as IRF-3, except for the utilization of the 180 bp bases in intron 6 adjacent to exon 6. The first 2,3 and 4 bases are a stop codon, which may produce a protein with a truncated C-terminal stoped at amino acids 327. Western blot analysis confirmed an expected 44 kDa strong band. The new inserted bases can be found in murine EST database, suggesting a conservative function in evolution. The functional luciferase assay showed that IRF-3c inhibited the IFN? promoter activity to (around) 40%～50% as that of control after Sendai virus infection. Conclusions The discovery of a new isoform of IRF-3 provides a new insight into the functional regulation of IRF-3 family. It is a dominant-negative inhibitor for interferon ? promoter activity in the virus infection pathway, provides a mechanism for the fine-tuning of the virus-induced activation of the interferon response, and prevents interferon ? from its overexpression and its toxic effects. It is worthwhile to explore the role of IRF-3c in the pathogenesis of human diseases using IRF-3c’s specific sequence.

Objective To explore the inhibitory effect of mutant influenza A viruses to the activation of interferon regulatory factor 3 (IRF-3). Methods HEK293 cells were infected with A/FM/1/47,A/HK/1/68, A/HK/1/68-MA20, A/HK/1/68-MA20C and positive control Sendai virus (SV). Whether the slowly moved phosphorylation form Ⅲ and Ⅳ of IRF-3 appeared or not was compared by Western blot in cells infected with these viruses. Wild type of NS1 from A/HK/1/68 and mutant NS1 from A/HK/1/68-MA20 were subcloned into pcDNA3.1-flag respectively. They were transfected in HEK 293 cells respectively. At 16 hours posttransfection, cells were infected with Sendai virus for 8 hours. Whole cell extracts were analyzed by Western blot and then probed with monoclonal flag antibody to check the expression of NS1, or with anti-IRF-3 to observe the inhibitory effects of the wild and mutant NS1 to the activated IRF-3. Luciferase assay was carried out by co-transfection with reporter plasmid, pGL2B with interferon ? promoter, and wild or mutant NS1 cDNA expression plasmid. SV was used to infect these cells after the co-transfection. Results Only less virulent A/HK/1/68-MA20 and positive control SV can activate IRF-3. Activated form Ⅲ and Ⅳ of IRF-3 began to appear 9 hours post infection (h.p.i), and most significant activated IRF-3 appeared 23 and 26 h.p.i. Sequence analysis of NS1 of MA20 revealed that nucleotide position number 94 is mutated from T to C, and amino acid at position number 23 is changed from valine to alanine. Co-transfected with wild type NS1 made form Ⅲ and Ⅳ of IRF-3 almost disappear, but not mutant NS1. In the luciferase functional analysis, wild type NS1 can inhibit the luciferase activity of IFN-? promoter, which was induced by SV, to around 1/10. Again no inhibitory effects was observed of mutant NS1 in the luciferase assay. Conclusion The mechanism that A/HK/1/68-MA20 can activate IRF-3 is that point mutant NS1 abolished the inhibitory function of NS1.

Objective To investigate the protection of metformin on kidney in non-diabetic hypertension elderly.Methods The patients was divided into two groups;one given nifedipine ,another given nifedipine plus metformin. Three months Later,the changes of body mass index (BMI), plasma insulin, urine protein, blood urea nitrogen(BUN), blood and urine ? 2-microglobulin(? 2-MG) were examined. Results Above-markers were reduced more remarkably in nifedipine plus metformin group than that in only nifedipine group(P

Objective To investigate the clinical significance of vascular endothelial growth factor (VEGF) levels in the serum of patients with acute myocardial infarction (AMI),and also to explore the relationship between VEGF and creatine kinase (CK) levels in AMI.Methods 25 patients with AMI and 12 controls were used for this study. Serum was isolated from peripheral blood on day 1, 5, 10 and15 after the onset of AMI. VEGF levels were measured by enzyme-linked immunosorbant assay (ELISA).Results Serum VEGF levels elevated gradually after the onset of AMI and reached a peak on day 10, which was significantly higher than that of control group (178 9?48 9)pg/ml vs(64 9?16 7)pg/ml,P

Objective To observe the dynamic change of the intimal proliferation in balloon-injured rabbit carotid arteries to explore the mechanism of restenosis. Methods 40 healthy male New Zealand white rabbits were randomly divided into experiment groupsⅠ、Ⅱ、Ⅲ and control group, each group containing 10 rabbits. Right carotid artery was injured with PTCA balloon in the experiment groups. 10 rabbits in each experiment group were killed on 1, 2 and 4 weeks after injury. Morphological change of injured intimae was examined by light microscope and analyzed using a computerized imaging analysis system. Results In experiment group, neointimal areas were significantly more than those in control group(P

Objective To investigate IL-18 expression of monocyte cells and the inhibition effect of fluvastation on its expression in patients with acute coronary syndrome(ACS). Methods Isolated monocyte cells from peripheral blood of patients with ACS were incubated with different concentrations of fluvastatin, and IL-18 mRNA level of the monocyte cells was detected by reverse transcription polymerase chain reaction(RT-PCR). Results The monocyte cells of peripheral blood in patients with ACS expressed IL-18 mRNA. IL-18 mRNA decreased from 54 1?9 9 to 50 1?12 6(P

Objective To observe the effect of anxiety and depression on cell adhesion molecules and fibrolytic activity in cardiac-pacing patients. Methods The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 were measured in 39 cardiac-pacing patients with anxiety or depression as rersearch group, 32 cases cardiac-pacing patients without any psychological problems as observation control group and 20 healthy subjects as normal control group. Result The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 in cardiac-pacing patients were significantly higher than those in normal control group, and their plasma levels in the patients with anxiety or depression were significantly higher than those in the patients without psychological problems. Conclusion There were significantly elevated cell adhesion molecules level and decreased fibrolytic activity in cardiac-pacing patients. The anxety and depression status could aggregate this condition.