1.Effect of octreotide combined with compound coenzyme on Th17/CD4+CD25+Foxp3+ Treg imbalance in patients with purulent appendicitis
Zifang TAN ; Wenli WU ; Jian WANG ; Shenghong JING
Chinese Journal of Biochemical Pharmaceutics 2015;37(7):95-97
Objective To analyse the effect of octreotide combined with compound coenzyme on Th17/CD4 +CD25 +Foxp3 + regulatory T cells imbalance in patients with purulent appendicitis.Methods 150 patients with acute suppurative appendicitis were randomly divided into experimental group and control group, 75 cases in each group.Patients of two groups underwent appendicitis laparoscopic resection, then the control group were given conventional treatment and experimental group were given subcutaneous injection of octreotide on the basis of conventional treatment.Th17, CD4 +CD25 +Foxp3 +regulation T cells ( Treg) , and Th17 /Treg balance were analyzed.ResuIts Compared with control group, Th17/Treg imbalance of experimental group recovered better: the proportion of Th17, Treg, and Th17/Treg ratio decreased (P<0.05); the expression levels of related transcription factor, RORγt and Foxp3 mRNA decreased (P<0.05); serum cytokine (IL-4, IL-6, IL-10, IL-17, IL-23 and TGF beta) and C-reactive protein (CRP) content decreased (P<0.05);serum interferon gamma (IFN-gamma) content increased (P<0.05).ConcIusion Octreotide combined with compound coenzyme could correct Th17/Treg imbalance in suppurative appendicitis patients, improve the immune function of patients and affect the prognosis of patients.
2.Study on the Protective Effect of Bilobalide on Ischemical Reperfusion Injury in Rats Undergoing Liver Transplantation
Shenghong JING ; Huicheng WANG ; Gang SU ; Yuzhuo FAN ; Binggang ZHOU
China Pharmacy 2007;0(33):-
OBJECTIVE:To observe the effect of Bilobalide on ischemical reperfusion injury in orthotopic liver transplantation model rats and to discuss its protective effect on ischemical reperfusion injury of rats undergoing liver transplantation.METHODS:The model of orthotopic liver transplantation was established by in rats by setting up the bridge between portal vein and left renal vein and building blood bypass by inlaying of canal in the inferior vena,after modeling,the rats were treated with Bilobalide,then levels of NO(active medium of blood vessel)and ET1 before ischemia and at 10 min,30min and 2h after reperfusion were determined by nitratase reduction method;serum levels of ALT,AST,ALP,LDH enzymology,ATP and MDA in the liver tissues were determined as well.The hepatic tissue sample was taken at 2h and fixed with formalin to be made into specimen for observation of the ultrastructure of liver cells and hepatic lobules under electron microscope.RESULTS:After being treated by Bilobalide,serum NO level was elevated and the pathological elevated levels of ALT,AST and LDH were brought down,the microcirculation in ischemical reperfusion injury rats was improved and the extent of damage in the ultrastructure of liver cells was lessened in rats.CONCLUSION:Bypass of portal vein and inferior vena cava prior liver transplantation is helpful for the prevention of ischemical reperfusion injury of liver.The balance of NO/ET1 is possibly a factor influencing the blood flow in the microcirculation of transplanted liver.Bilobalide was proved to be of protective effect on ischemical reperfusion injury in rats.
3.Survey on health status and utilization of health service among native herdsmen in the Karakorum-Kunlun Mountains high altitude border areas
Juncai ZHANG ; Chaoguang JIANG ; Shenghong YANG ; Yinhu WANG ; Haijun YANG ; Hongyun WANG ; Jing JIN ; Chuanrong WANG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(3):321-323
Objective To analyze health status and characters of health service demand and utilization among farmers and herdsmen in Kangxiwa and Saitula border areas from the altitude of 3700m to 4300m for offered scientific advices to the health management institutions for optimizing the allocation of health resources.Methods A total of 51 families in the Kangxiwa and Saitula areas in Karakorum-Kunlun Mountains were randomly chosen with stratified sampling method for the family health survey.Physical and biochemical examination were carried out for each herdsman.Results Among the participants,the most disease the two-week medical consultation rate was 1.74%.the hospitalization rate was 0.98%.The two-week medical consultation and hospitalization rate of herds were lower than the national and Xinjiang level for rural area.Herdsmen's doctor poor accessibility and low-income may be the main reasons for the underutilization of health services ;The difficult situation of herdsmen has been initially improved after participating in the new rural cooperative medical care.Conclusion The health status of native herdsmen in Kara Kunlun mountains was poor,and the provision of health services cannot meet the demand for health service of native herdsmen.It's necessary to improve efficiency of utilization of health service and optimize the allocation of health resources.
4.Components in melanoma cytoplasm might induce murine BMSCs transformation and expression of melan-A.
Jing, MA ; Junjuan, SHI ; Jianchao, WANG ; Jun, LIU ; Ke, WU ; Qilin, AO ; Zhuoya, LIU ; Xiaoli, WANG ; Shenghong, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(5):663-6
This study explored the possibility that the components in melanoma cytoplasm induce murine BMSCs transformation and expression of Melan-A by morphologically observing the changes of BMSCs and immunocytochemically detecting Melan-A in the cells after culturing BMSCs in medium containing melanoma cytoplasm components (MCC). MCC of B16 melanoma cells was prepared and BMSCs were cultured and induced by adding the MCC into culture medium. The cells were morphologically observed and Melan-A was immunohistochemically detected to confirm BMSCs transformation. MCC-induced BMSCs underwent morphological changes. A number of melanin granules appeared in the cytoplasm of the cells and some were released into surrounding areas. Several cells that might come from one cell formed a cluster, and their granules, together with those secreted by other induced BMSCs, formed a so-called "sphere-formed structure". The induced BMSCs expressed Melan-A. We are led to conclude that there might be some factors in the cytoplasm of melanoma cells that might induce BMSCs transformation toward melanogenic cell, or even melanoma.
5.Analysis common gene mutation spots of 127 non-syndromic deafness natients in Guangxi Drovince.
Shuixia LIU ; Liang XU ; Bowen CHEN ; Min LIU ; Shenghong QU ; Jianping LIANG ; Fengzhu TANG ; Min SHI ; Lu PENG ; Yan JING ; Fengti LI ; Youqiong LIANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(22):1954-1958
OBJECTIVE:
To investigate the mutation characteristics of common deafness gene from 127 non-syndromic hearing loss patients in Guangxi province.
METHOD:
Deafness-related gene mutations detection kit was used to detect 15 mutation sites in four deafness-associated genes, and a total of 127 hearing impaired patients were tested. The samples that could not be diagnosed with DNA microarray were subjected to PCR and sequenced to detect other mutations.
RESULT
Among the 127 patients with non-syndromic deafness, the total mutation rate is 8.66% (11/127), including GJB2 235delC homozygous in 3 cases (2.36%), 235delC single heterozygous mutation in 2 cases (1.57%), GJB2 235delC and 109 A > G mutations in 2 cases (1.57%); SLC26A4 1229C > T homozygous in 1 case (0.79%), IVS7-2A > G, IVS11 + 47T > C and 15448insC mutaion in 2 cases (1.57%); mitochondrial 12S rRNA gene mutations were not detected. The result indicates that GJB2 and SLC26A4 were the main genes in this study, and the mutation rate is significantly lower than the national average level. Three new mutations (SLC26A4 IVS11 + 47T > C,1548insC and GJB2 109A > G) were found. There may be rare mutations among sites or genes associated with deafness in Guangxi.
China
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Connexin 26
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Connexins
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genetics
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DNA Mutational Analysis
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Deafness
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genetics
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Heterozygote
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Homozygote
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Humans
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Membrane Transport Proteins
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genetics
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Mutation
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Oligonucleotide Array Sequence Analysis
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Polymerase Chain Reaction
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RNA, Ribosomal
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genetics
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Sulfate Transporters
6.Components in Melanoma Cytoplasm Might Induce Murine BMSCs Transformation and Expression of Melan-A
MA JING ; SHI JUNJUAN ; WANG JIANCHAO ; LIU JUN ; WU KE ; AO QILIN ; LIU ZHUOYA ; WANG XIAOLI ; LIU SHENGHONG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(5):663-666
This study explored the possibility that the components in melanoma cytoplasm induce murine BMSCs transformation and expression of Melan-A by morphologically observing the changes of BMSCs and immunocytochemically detecting Melan-A in the cells after culturing BMSCs in medium containing melanoma cytoplasm components (MCC).MCC of B16 melanoma cells was prepared and BMSCs were cultured and induced by adding the MCC into culture medium.The cells were morphologically observed and Melan-A was immunohistochemically detected to confirm BMSCs transformation.MCC-induced BMSCs underwent morphological changes.A number of melanin granules appeared in the cytoplasm of the cells and some were released into surrounding areas.Several cells that might come from one cell formed a cluster,and their granules,together with those secreted by other induced BMSCs,formed a so-called “sphere-formed structure”.The induced BMSCs expressed Melan-A.We are led to conclude that there might be some factors in the cytoplasm of melanoma cells that might induce BMSCs transformation toward melanogenic cell,or even melanoma.
7.High-affinity glutamate transporters and chronic pain
Hua ZHANG ; 甘肃省骨关节疾病研究重点实验室 ; Shenghong WANG ; Qiqi XIE ; Wenzhou LI ; Weidong SHI ; Jinglin MA ; Jing WANG
Journal of Central South University(Medical Sciences) 2017;42(9):1094-1099
Glutamate serves as a major excitatory neurotransmitter in the mammalian central nervous system and is stored in synaptic deft by an uptake system that is dependent on the high-affinity glutamate transporters (ETTAs),which locate in the plasma membrane of glial cells and neurons.ETTAs can rapidly terminate the action of glutamate and maintain its normal physiological functions.If the content or function of glutamate transporters is abnormal,it can result in many physiological dysfunctions.Studies have demonstrated that high-affinity glutamate transporters play an important role in the development of chronic pain,which might be a new therapeutic target for the pain.