Objective To evaluate the clinical application value of influence of chylemia specimen before and after blood plasma exchange on the detection results of hematological analyzer.Methods 22 chylemia specimens were collected,then the hematological analyzer was used to test the chylemia specimens before and after blood plasma exchange,and the results were compared with those detected by the manual microscopic counting.Results The detection results of WBC,NEU,LY,MO,RBC,HGB and PLT in the non-chylemia specimens and the original blood specimens before blood plasma exchange had no statistically significant differences (P >0.05);in 22 cases of chylemia specimens,the WBC count and classification,and the mean value of HGB and PLT before and after blood plasma exchange in the chylemia specimens had statistically significant differences (P <0.05),the WBC count and clas-sification,and PLT mean value after blood plasma exchange in the chylemia specimens by the hematological analyzer had good corre-lation with those detectd by the microscopic counting method(r2 =0.936,r2 =0.918).In the triglyceride interference test,the WBC count and classification,and the mean value of HGB and PLT could be changed with the increase of the triglyceride concentration. Conclusion Adopting the blood plasma exchange method could obviously improve the influence of chylemia specimens on the detec-tion results by the hematological analyzer and provide the accurate results for clinic.