Objective To investigate the pathogens,clinical characteristic and therapeutic method of acute kidney injury(AKI) Methods The morbidity,composition of pathogeny,staging and prognosis of 624 cases with AKI recruited by our department from January 1999 to December 2009,to evaluate the impacts of possible predisposing factors.Results The mortality rate of AKI was 19.6％(122/624),The mortality in the staging of exhaustion,damage,risk were 37.8％,33.4％ and 6.7％,respectively.Of the 624 patients there were 21 cases (3.4％) discharged due to exacerbation,29 cases (4.7％) maintained the status quo,and 452 cases (72.4％) cured or improved;Among 138 patients with blood purification there vere 116 cascs(84.1 ％)cured or improved and 22 cases (90.9％) with long term blood purification cured or improved.The pathogenyof the non-elderly group (＜60 years old)were original diseases and collapse,and the pathogeny of the elderly group (≥60 years old)were original diseases.Conclusions AKI is serious with high incidence and mortality in hospitalized patients.The efficacy of long-term blood purification is better than common treatment.Early prevention,early diagnosis and active intervention will help to increase survival rate of AKI aud improve prognosis.

OBJECTIVETo investigate the effect of human serum albumin (HSA) on cell attachment of human gingival epithelial cells (HGE).

METHODSHGE were primary cultured with keratinocyte serum-free medium (KSFM) and dispase. The cultured cells were immunohistochemically stained by monoclonal anti-pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed.

RESULTSThe results showed significant decrease in cell numbers within 8 hours after HGE were inoculated, in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference.

CONCLUSIONSHSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.

Cell Adhesion ; drug effects ; Cell Count ; Cell Division ; drug effects ; Cells, Cultured ; Epithelial Cells ; cytology ; drug effects ; Gingiva ; cytology ; drug effects ; Humans ; Polystyrenes ; Serum Albumin ; pharmacology