1.Asymptomatic Leishmania Infection in Human Population of Wenxian County,Gansu Province
Junyun WANG ; Yu FENG ; Chunhua GAO ; Changfa JIN ; Shengbang CHEN ; Chouji ZHANG ; Jinping HE ; Chenming YANG ; Yuetao YANG ; Yifang BAO
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(01):-
Objective To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County,Gansu Province,and to evaluate the tests used.Methods Blood samples were tested by PCR using two pairs of primers,RV1-RV2 and K13A-K13B,for detecting Leishmania-specific DNA.ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies.Results The positive rate of PCR,ELISA and rK39-dipstick was 30.9%(83/269),24.2%(65/269) and 0(0/269) respectively.Conclusion The prevalence of asymptomatic infection of L.infantum in humans is high in the area.PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.
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