Carcinoma, Hepatocellular ; therapy ; Humans ; Liver Neoplasms ; therapy

Combined Modality Therapy ; Humans ; Liver Neoplasms ; therapy

Objective To explore the influence of cirrhotic portal hypertension and its complications on liver transplantation. Methods The literatures of the recent years on influence of hepatic cirrhotic portal hypertension on liver transplantation were reviewed. Results Splenomegaly, hypersplenism, portal vein thrombosis, portosystemic shunt and collateral flow in cirrhotic patients will increase the difficulty of liver transplantation and lead to more postoperative complications. Appropriate handling of these conditions can achieve a higher success rate of liver transplantation. Conclusion Correct management of end-stage cirrhotic portal hypertension and its complications can expand the indications of liver transplantation and improve long-term survival rates.

Carcinoma, Hepatocellular ; therapy ; Chemoembolization, Therapeutic ; Humans ; Liver Neoplasms ; therapy

Carcinoma, Hepatocellular ; therapy ; Drug Delivery Systems ; methods ; trends ; Genetic Therapy ; Humans ; Liver Neoplasms ; therapy ; Receptor, Epidermal Growth Factor ; administration & dosage

Antineoplastic Agents ; therapeutic use ; Carcinoma, Hepatocellular ; pathology ; surgery ; therapy ; Catheter Ablation ; methods ; Chemoembolization, Therapeutic ; Combined Modality Therapy ; Hepatectomy ; methods ; Hepatic Artery ; Humans ; Lung Neoplasms ; pathology ; surgery ; therapy ; Neoplasm Staging ; Radiotherapy, Conformal

Objective To observe the effect of asarone injection on terms of airway inflammation factor in asthmatic children.(Metho)ds Sixty asthmatic children were chosen and divided randomly into methotrexate group and control group.In the methotrexate group,intravenous injection with 2 times per day was given.The concentration of the injection solution was 0.01%,which was mixed by(0.5 mg/kg) asarone injection and 10% glucose injection.In control group,intravenous injection once per day was given.The solution consisted of 4-6 mg/kg aminophyline injection and 100 mL 5% glucose injection.Every 7 continuous days was taken as one process stage.Two process stages were observed during the experiments.Results After two weeks,the eosinophils and the macrophage were changed significantly(P

Anastomosis, Surgical ; methods ; Humans ; Organ Transplantation ; adverse effects ; methods ; Postoperative Complications ; etiology ; surgery ; Vascular Surgical Procedures ; methods

To further analyze immunoglobulin (Ig) properties of panel reactive antibodies (PRA) and their potential harmful effects on allogeneic renal transplants. Methods Since dithiothreitol (DTT) is able to crack disulphuric bond of Ig and to depoly-merize IgM with heavy molecular weight, PRA study was performed with treatment of DTT. Thus, a DTT-ERA method was established, which can identify immunoglobulin class in sera of high PRA patients. Results Among 701 recipients, whose sera were positive by the standard PRA, the positive rate of DTT-PRA was 33.2% . Besides, positive PRA patients could be divided into three groups according to their sera's sensitivity to DTT: Group 1 consisted of patients whose sera contained exclusively IgM antibodies; Group 2 consisted of patients whose sera contained of IgG antibodies only; Group 3 consisted of patients whose sera were found to contain both IgM class antibodies and IgG antibodies. Conclusion IgM class antibodies are not associated with posttrans-plant rejection, while IgG antibodies alone and mixture of IgG and IgM antibodies may mediate acute, or even hyperacute rejection.

Objective To investigate the effects of passaging on the proliferation,differentiation and apoptosis of neural stem cells.Methods Neural stem cells both from prime culture(P0) and twice-passaged culture(P2) were tested by enzyme immunoassay for proliferation and fluorescent activated cell sorting for apoptosis by Annexin V-FITC and propidium iodide.After being enduced to differentiation for 7 days,they were detected by the neuronal marker MAP2 and the astrocyte marker GFAP with nuclei staining by Hoechst 33342 additionally.The ratios of MAP2+/Hoechst+ and GFAP+/Hoechst+ were calculated.Results The absorbance of NSCs from P0 and P2 is 1.1899?0.0485(n=13)and 0.3526?0.1053(n=11)(P=0.000).Analyses of apoptosis using cytoflurometry are 40.91%?12.74% for P0(n=8)and 24.31%?13.01% for P2(n=9,P=0.018).The ratios of MAP2+/Hoechst+ and GFAP+/Hoechst+ of NSCs from P0 were 58.81%?9.69%(n=10)和22.85%?4.36%(n=10) while the ones for P2 were 27.84%?5.70%(n=10)and 49.32%?6.95%(n=10,P=0.000).Conclusions By passaging the proliferation and apoptosis of NSCs decreases and they differentiate into less neurons and more astrocytes.