Objective To investigate the expression and clinical significance of fibroblast growth factor-2 (FGF-2) and human teclomerase reverse transcriptase (hTERT) in non-small cell lung cancer (NSCLC) tissues and analyze the correlation between them.Methods The expression of FGF-2 and hTERT were detected by immunohistochemistry in 90 NSCLC tissues and 20 non-lung carcinoma tissues.The correlation between the expression and clinicopathological features was also analyzed.Results The positive expressions of FGF-2(66.7％ ) and hTERT (75.6％) in NSCLC tissues were significantly higher than that in nonlung carcinoma tissues (15％ and 20％,respectively),P ＜0.01.The expression of FGF-2 and hTERT were significantly related to TNM stages and lymph node metastasis (P ＜ 0.01 ),but not to histological types,sex and age of NSCLC patients ( P ＞ 0.05 ).A positive correlation was found between the expressions of FGF-2 and hTERT in NSCLC(P ＜ 0.01 ).Conclusion The expression of FGF-2 and hTERT in NSCLC,which can be regarded as important references for prognosis of NSCLC,play important roles in the initiation and development of NSCLC.

Chimera

Objective To observe age-dependent feature of damage of hippocampus to different maturational stages rats after kindling repeated seizures.Methods The effects of 5 daily pentylenetetrazol-induced convulsions in different rats beginning at postnatal day 10,20,60(P10,P20,P60)were evaluated.In the 3 groups,Thionin staining method was utilized to observe morphological changes and cell counting of dentate granule cells,CA3,CA1,and hilar neurons.Timm's method of silver sulfide staining was adopted to observe the mossy fiber sprouting.Results 1.Cell counting of CA1,CA3 and hilar neurons in P10 and P20 groups demonstrated no differences from controls in rats,whereas P60 with daily seizures had a significant decrease in CA1,CA3 neurons(8.22?1.88,5.62?1.68 vs 6.31?1.50,3.62?1.40)(t=2.246,2.587 Pa

Objective To fully assess the status of cancer chemotherapy quality of secondary and tertiary hospitals in Shanghai City in order to improve the quality control system of cancer chemotherapy.Method According to the supervision plan of Shanghai Cancer Chemotherapy Quality Control Center,all of the 103 secondary and tertiary hospitals in Shanghai City which administrated chemotherapy to cancer patients have been inspected in 2015.SPSS13.0 was used for data aggregation and analysis.Result The cancer chemotherapy quality was significantly different between the secondary and tertiary hospitals,as well as between the oncoiogy and non-oncology departments,the main problems were as follow.:the informed consent content was incomplete (33.04％);pathological diagnosis was incomplete or missing (15.50％);tumor stage was not standardized or no staging (56.27％);the chemotherapy purposes (42.44％) and the chemotherapy indications (37.47％) and contraindications (12.21％) were incomplete or missing;the evaluation of chemotherapy efficacy was incomplete or not evaluated (24.10％);the mid-term assessment was incomplete or not evaluated (27.72％);admission history (16.28％) and records of the chemotherapy day (32.04％) were incomplete or missing.Conclusion The quality of medical records of cancer chem0therapy in Shanghai City is significantly related to hospital level and specialty.The hospital authorities should learn the quality control standards based on their own problems.

To explore the mode of matrix and cell injury of articular cartilage subjected to high stress mechanical loads with a low rate of loading. Loads characterized by a constant stress rate of 0.26MPa/s to a peak stress between 2MPa and 11MPa being applied to the adult bovine articular cartilage explants was designated as the test groups. No mechanical loads were applied to the control group. All the explants were maintained in culture for the next few days after loads. The spatial patterns and severity of chondrocyte injury were determined using fluorescein diacetate and propidium iodide. Matrix damages were assessed by inspection of the microstructure of the collagen fiber network under SEM. When the peak stress was 5MPa and higher, the rate of cell death in the explants of test groups was significantly greater compared with controls, and the greater peak stress applied, the higher percentage of dead versus total cells was documented, and a more diffuse distribution of dead cells was observed. When the peak stress reached 9MPa, cell death involving full depth of the cartilage was observed in test groups . Damages to the collagen fiber network were not observed both in test and control groups. The results indicated that high stress mechanical loads with a low rate of loading resulted in more severe chonrocytes damage rather than collapse of collagen fiber network.

This paper reports the perioperative care of a patient undergoing the bilateral posterior ventral globus pallidus deep brain stimulation treatment for neuroacanthocytosis. The nursing points mainly included psychological care of the patient before surgery,preoperative preparation,postoperative care and special nursing before and after deep brain stimulation opening. The patient's dance-like involuntary movements and compulsive behavior reduced significantly after the treatment with no complications.

Objective To investigate the effects of nutritional support on the hypermetabolism of patients with digestive tract fistula complicated with severe abdominal infection. Methods Twenty-nine patients with digestive tract fistula complicated with severe abdominal infection were administered parenteral nutrition (PN) for 4 to 8 days, and then followed by enteral nutrition (EN) or EN + PN. The body weight, serum albumin, transferrin, prealbumin, fibronectin were monitored before and on the 10th and 28th day after nutritional support. Results The levels of serum prealbumin and fibronectin were increased significantly on the 10th day after nutritional support (t = 3.72, 3.52, P <0.01). The body weight, the levels of serum albumin, transferrin, prealbumin and fibronectin were significantly higher than those before nutritional support (t=3.97, 6.57, 7.09, 3.51, 3.58, P<0.01). Conclusion Nutritional support is effective in alleviating hypermetabolism of patients with digestive tract fistula complicated with severe abdominal infection.

Oral fluid resuscitation in early period of hypovolemic shock is an important measure in the treatment of casualties in the battlefield as well as in mass casualties in lieu of the means of establishing a venous line. The main tasks in the study of oral rehydration resuscitation are the proposition of appropriate prescriptions of the liquids to be given and methods of administration on the basis of a clear elucidation of the mechanism underlying the function of the digestive system in regard to transportation and absorption of the given ingredients. The aim of the study is to fully replenish maximal amount of fluid containing glucose and electrolytes in a convenient, expeditious, and effective way in a minimal span of time, in order to increase circulating blood volume. At the same time, ischemia and tolerance to oral fluids of the gastro-intestinal tract should be improved, electrolyte imbalance and incidence of secondary infection should be alleviated, and finally hypovolemic shock is corrected, so that the victim is kept alive and prepared for further definitive surgical intervention.

Objective To analyse the pathologic changes and MRI appearances of mitrial atresia (MA). Methods MRI appearances of 5 cases with MA confirmed by operation were reviewed and compared with angiocardiography (CAG). Results MRI demonstrated the pathologic characteristics of three types of MA according to the traditional classification:biventricular hearts with imperforate mitral valve (1 case),univentricular heart of right ventricular type with absent left atrioventricular connection (3 cases) and univentricular heart of left ventricular type with imperforate mitral valve (1 case).MRI still provided anatomic information of associated anomalies including mainly aneurysms of atrial septum (1 case),total anomalous pulmonary venous drainge into superior vena cava (1 case),absent left pulmonary artery (1 case) and pulmonary stenosis (4 cases). The right atrial and(or) ventricular angiocardiograms in the 5 cases could not display the anatomic morphology of the malformation,with exception of one case only. Conclusion MRI is valuable in diagnosis of MA.

Purpose:To evaluate the feasibility of irradiat ion to the metastatic retroperitoneal lymph nodes using three-dimensional confo rmal radiotherapy (3-DCRT) and to assess the irradiation side effects. Methods:From Jun. 1997 to Sep. 2000, 52 patients of metastatic retroperitoneal lymph nodes were treated with 3-DCRT.These patients had receive d radical therapy for their primary tumors. 50 of them had been proved by pathol ogical examination. Radiotherapy was designed by Elekta company’s 3-D treatmen t plan. Dose volume histography (DVH) was used to verify and optimize the radiat ion plan. Using 5～6 conformal portals,the total irradiation dose was 40 Gy an d delivered at 4～5Gy per fraction every other day,to a total of 8～10 fractions in 16～20 days. The immediate responses and side effects were evaluated accordi ng to the WHO and RTOG criterion system. Results:According to Karnofsky criterion system the median scor e was raised 21.3. Three months after irradiation, the complete ,partial and tot al response rates were 38.6%(27/70), 54.3%(38/70)and 92.9%(65/70),respect ively. The early response was related to the target volume and pathological clas sification. The smaller the target volume, the better the response(? 2 = 1 5.211,P=0.004).The anaplastic carcinoma cases had better results than the squamous carcinomas and adenocarcinomas(? 2 =13.455,P=0.009).The 1- ,2- and 3- year survival rates were 59.6%(31/52)、23.1%(12/52)and 9.6%(5/ 52),respectively. Conclusions:Three dimensional conformal radiotherapy can be con sidered as an effective and feasible approach for the treatment of metastatic r etroperitoneal lymph nodes,and no serious complications were observed.