Cytokine induced killer (CIK) cells are the main immune cells for tumor immnuotherapy.Relevant basic researches show that CIK cells have significant antitumor effects for various hematologic tumor and solid tumors.Clinical studies confirm that CIK cells-based therapy have exact therapeutic effect and mild adverse reaction for tumor patients.So CIK cells-based therapy is a promising and safe method and it is important to establish standards for cultivating and using CIK cells.

Objective　To study the factors effecting the formation of the ventricular aneurysm post myocardial infarction (MI) and evaluate the diagnostic methods.Methods　23 MI patients with ventricular aneurysm confirmed by left ventricular angiography were observed for electrocardiogram (ECG), echocardiogram (ECHO), coronary disease and collateral vessels formation. Results　All patients had LAD disease, 65.2% patients had three coronary disease, and those with class Ⅲ collateral vessels circulation only 17.4%. Compared to ECHO by which only 4 patients were found aneurysm, the positive rate by ECG was 82.6%. Conclusion　Patients with multiple coronary disease and without enough collateral vessels are expected to form ventricular aneurysm. ECG is more sensitive than ECHO for diagnosis of ventricular aneurysm post MI

Low molecular weight heparin(LMWH)is a kind of anticoagulant used in both the prevention of clot formation in the blood vessels(thrombosis)and the treatment of conditions caused by clot formation or embolization.They are agents formulated from chemical or enzymatic depolymerization of unfractionated heparin.In the past decade,LMWH has been extensively evaluated in many clinical settings.The applications of LMWH in recent years are reviewed.

Objective To analyze the morbidity rate and clinical etiology distribution of secondary thrombocytosis(ST ) in chil‐dren in order to guide the clinical treatment .Methods The clinical data of 170 children patients with ST in hospitals were retro‐spectively analyzed for investigating its etiology distribution ,total morbidity and onset situation in different age periods . Results The primary etiologies of ST mainly included the infectious diseases(especially respiratory tract) ,anemia ,immune disea‐ses ,drugs ,surgical disease ,etc .Thrombocytosis caused by neonatal diseases also were common .The total morbidity rates of ST in children was about 3 .37% and which in newborns ,infants and children aged over 3 years were 2 .48% ,3 .78% and 1 .99% respec‐tively ,the difference was statistically significant(P<0 .5) .Conclusion ST is one of the common complications in children ,especial‐ly in children aged under 3 years old .The etiology of ST is various ,which are dominated by respiratory and digestive tract infec‐tions .Children aged under 3 years old ,especially infants and young children with respiratory tract infection are easier to suffer from complicating thrombocytosis .

Objective To investigate the roles of hypoxia regulated genes/protein in chemical hypoxia preconditioning in differentiated SH-SY5Y cells.Methods Differentiated SH-SY5Y cells were randomly divided into control group,chemical hypoxic preconditioning group(50 ?mol/L CoCl2 preconditioning for 3 h,normal culture for 1 h,then in 2%O2 for 28 h) and hypoxia group(in 2%O2 for 28 h).RT-PCR was used to examine the mRNA expressions of VEGF,GLUT-1,EPO,LDH-A.Further evaluation of the potential neuroprotective effect of VEGF was done by investigating the effect of recombinant human VEGF on subsequent hypoxia injury.Results The mRNA levels of GLUT-1,EPO increased in the preconditioning group as compared with those in the hypoxia group(P

China ; epidemiology ; Humans ; Pneumoconiosis ; epidemiology ; prevention & control ; Population Surveillance

Objective To investigate the changs of serum and urine vascular endothelial growth factor(VEGF) in children with steroid-sensitive nephrotic syndrome(SSNS) at their active and remission stage.Methods Serum and morning urine levels of VEGF were assayed by Liquichip in 30 patients with SSNS at the active and remission stage and 30 normal age-and sex-matched controls.Results Serum levels of VEGF in patients at the active stage [(186.62?106.21) ng/L] were significantly higher those that at the remission stage [(118.75?73.08) ng/L] and than those in control group [(108.64?54.75) ng/L](P0.05).Morning urine levels of VEGF in patients at the active stage [(201.66?100.46) ng/L] were significantly higher than those at the remission stage [(116.35?55.99) ng/L] and than those in control group [(99.94?42.07) ng/L](P0.05).Conclusions Serum and morning urine levels of VEGF are significantly elevated in patients with SSNS,however they are significantly lower after the treatment of steroid.There is no obvious difference between the patients at the remission stage and healthy controls.VEGF involves pathogenetically and physiologically in SSNS during its onset and growth.

Objective To investigate the quality control of the next-generation sequencing data by analyzing some im-portant problems with quality control in order to provide high-quality data for the downstream data analysis .Method The software Fastq_clean was developed to clean the data and to statistically analyze the quality of the data.Result Using a Chrysanthemum transcriptome dataset , the function of the Fastq_clean software was illustrated .Conclusion Based on the Bioconducter package , the Fastq_clean software can accurately remove low quality nucleotides and Ns , adapter contamina-tion, possible rRNA and virus contamination .Moreover, it can keep the largest possible amount of high-quality data reads.

ObjectiveTo compare the mRNA and protein expression levels ofγ-aminobutyric acid receptor B2 ( GABABR2 ) in brain regions of male rats with high level aggressive behaviors and low level aggressive behaviors respectively,and provide clues for exploring mechanism of GABA in aggressive behaviors.MethodsWistar male rats were randomly divided into two groups:the normal group and the aggressive behavior group.Then social isolation and resident intruder stresses were used to establish high level and low level aggressive behavior in the aggressive behavior group.The mRNA and protein level of GABABR2 in parietal cortex,prefrontal cortex,hypothalamus and hippocampus of the three groups rats ( n=10 in each group) were detected using RT-PCR and Western blot respectively.ResultsThe GABABRB22 RT-PCR/Western blot relative integrated optical density of parietal cortex,prefrontal cortex,hypothalamus and hippocampus in the normal group rats respectively were.Those of the above four brain regions in high aggressive behavior group rats respectively were ( 0.507 ± 0.049/0.626 ±0.038 ),(0.609 ± 0.049/0.652 ± 0.010 ),( 0.359 ± 0.030/0.731 ± 0.044 ) and ( 0.296 ± 0.054/0.452 ±0.079) were significantly lower (P＜0.05) compared with the normal group rats.In the low aggressive behavior group rats,the GABABRB2 RT-PCR/Western blot relative integrated optical density of parietal cortex and hippocampus increased statistically(P＜ 0.05 ),while those of prefrontal cortex and hypothalamus decreased obviously (P ＜ 0.05 ).all in comparison with the normal group rats.Conclusion Different expression levels of GABABR2 in parietal cortex,prefrontal cortex,hypothalamus and hippocampus are relative to aggressive behaviors,which might be one of the mechanism for GABA in aggressive behaviors.