To reveal the mechanism of Cu enrichment of alfalfa, in-situ micro-X-ray fluorescence spectrometry (μ-XRF) and fractional extraction were used to explore in-situ Cu distribution information in alfalfa seedlings and different combination forms of Cu in organs.The results showed that alfalfa roots were enriched with Cu up to 12.06 mg/g, which was 8 times of stem and 4.9 times of leaves.The in-situ μ-XRF result showed that the root of alfalfa was the main site of enrichment of Cu, and there was a barrier of Cu at the rhizome junction to alleviate the toxic effect of excess Cu on the shoots.Excess Cu (more than 50 μmol/L) also inhibited the uptake of Zn and Ca in alfalfa, enhanced the uptake of Fe in alfalfa, but had no obvious effect on the uptake of K and Mn.Plant fractional extraction showed that the Cu in root cells was mainly fixed in the form of insoluble residues (41%) and cell wall chelate (20%), while in the stem, Cu existed as four forms including hydrophobic protein binding, cell wall binding state, residual state and water-soluble state, which further reduce the excess transport of Cu to the leaves.In the leaves, excess Cu in the leaf cells was mainly in the vacuole and insoluble residue to achieve tolerance and detoxification to Cu.

Objective To explore the related factors that affect the incidence of uterine fibroids to provide a theoretical basis for the clinical diagnosis and prevention.Methods Based on the clinical data selected from June 2014 to July 2016 in pathology of our hospital,159 cases of patients were confirmed with uterine fibroids,a comparative study was employed and the single factor test and the logistic regression analysis were both used to analyze the related risk factors that affecting the incidence of uterine fibroids.Results There were all significant level (P ＜ 0.05) between case and control groups in 40 years to 50 years,the number of abortions (≥2) as well as gynecological diseases,which were the independent factors for the incidence of uterine fibroids.In the present,the regression coefficients of former two factors were 0.186 (P ＜ O.05),and 0.584 (P ＜ 0.05),respectively.The corresponding regression coefficients of breast hyperplasia,vaginitis,cervicitis,endometritis and pelvic inflammatory disease,and other gynecological diseases and gynecological diseases were 0.221 (P ＜ 0.05),O.363 (P ＜ 0.05),O.539 (P ＜ 0.05),0.361 (P ＜ 0.05),and 0338 (P ＜ 0.05),respectively.It suggests that these were independent factors affecting the incidence of uterine fibroids.Conclusions The more the increasing age and the number of abortions,kinds of gynecological diseases infected will increase the risk of patients suffering from uterine fibroids.

Objective To observe the short-term effect of alprostadil combined with torasemide in the treatment of intractable heart failure.Methods Thirty cases of patients with intractable heart failure and ineffective in routine therapy were given alprostadil and torasemide treatment,with alprostadil 20 mg in 5％ glucose or 0.9％ sodium chloride solution 100 ml intravenous infusion once a day and torasemide 20-40 mg intravenous twice a day 3 days later torasemide dosage was adjusted according to the disease,and the remaining conventional anti-heart failure therapy unchanged.Course was one week.The blood pressure,body weight,urine output,creatinine,electrolytes,N-terminal pro-brain natriuretic peptide (NT-proBNP)and left ventricular ejection fraction (LVEF) before and after treatment was observed.Results After treatment,clinical symptoms of dyspnea,edema,pulmonary rales,such as wet and dry significantly improved in all patients.During the course of the treatment,3 patients appeared mild hypokalemia,and potassium was promptly corrected.Body weight after treatment reduced compared with that before treatment [(63.8 ± 7.6) kg vs.(82.6 ± 10.7) kg],urine output increased after treatment compared with that before treatment [(2 328.3 ±367.8) ml/d vs.(568.7 ± 104.6) ml/d],and the differences were statistically significant (P ＜ 0.01).Systolic blood pressure,diastohc blood pressure difference was not statistically significant after treatment,compared with that before treatment (P ＞ 0.05).LVEF after treatment increased compared with that before treatment [(44.5 ± 8.3)％ vs.(31.9 ± 10.2)％],serum creatinine levels reduced [(97.8 ± 18.6) μmol/L vs.(143.8 ±21.7) μmol/L],and the difference was statistically significant (P＜ 0.05) ; NT-proBNP after treatment reduced compared with that before treatment [(567.4 ± 212.3) ng/L vs.(2 726.5 ± 525.3) ng/L],and the difference was statistically significant(P ＜ 0.01).Conclusion Alprostadil combined with torasemide treatment can quickly and effectively improve symptoms and help improve heart and kidney function in intractable heart failure,and has no significant adverse reactions.

Objective To study the distribution of drug resistance mutations and genotypes in the patients with chronic hepatitis B(CHB)in Taicang area.Methods 350 cases of CHB were selected and detect the drug resistance mutations and genotypes before using nucleoside(acid)anti-viral drugs.The correlation between the drug resistance mutations with genotypes in CHB patients was analyzed.Results Among 350 cases of CHB,the distribution of genotypes was genotype B in 172 cases,accounting for 49.14%, genotype C in 164 cases,accounting for 46.85%,genotype D in 9 cases,accounting for 2.57%,mixed genotype of B and C in 5 ca-ses,accounting for 1.42%,no other genotypes were found.In this study,the drug resistance mutations were detected in 58 cases,in-cluding 22 cases of genotype B,35 cases of genotype C and 1 case of genotype D,no drug resistance mutations were found in mixed genotype B and C.The drug resistance mutations sites:lamivudine resistance-associated mutations in 36 cases(10.28%),and adefo-vir resistance-associated mutations in 16 cases(4.57%)and entecavir associated resistance mutations in 6 cases(1.71%).Conclusion The genotype of CHB patients in Taicang area was dominated by genotype B and C,which accounting for more than 90% and the proportion of these two kinds of genotype is similar.The proportion of drug resistance mutation occurrence in the patients with gen-otype C is higher than that with genotype B.The lamivudine resistance-associated mutation has the highest proportion of drug re-sistance occurrence.It is suggested that the CHB patients with undefined medication history and recurrence treatment should be per-formed the drug resistance detection for selecting the targeted therapeutic schemes before accepting nucleoside(acid)drug therapy.

Constant direct current stimulation has facilitation for cellular growth of many histiocytes.This paper primarily introduces the systemic and detailed design of the software as well as its achievement.The system can accomplish controlling action of peripheral circuitry through computer interface.In this way,it can run stably,harmoniously and safely.

BACKGROUND: 1200009K10 gene is from the lung cDNA pool of adult mouse and announced in January 2002, but its function is still not known.The predicted protein has five domains: Kelch motif, Kelch domain, BTB/POZ domain, BTB domain and β propeller domain, all of which are involved in protein-protein interactions and some enzymatic activities.OBJECTIVE: To explore the correlation between the expression of 1200009K10 gene and retinitis pigmentosa in mice.DESIGN: Observational and comparative trial.SETTING: Institute of Zhongshan Ophthalmic Center of Sun Yat-sen University.MATERIALS: The rd mice, rds mice and passage mice from C3B mice as normal control were purchased from Jackson Laboratory (Bar Harbor,Maine 04609, USA), and raised in second class unit of Experimental Animal Center of Sun Yat-sen University.METHODS: The retinal RNA of mice was extracted and isolated and 1200009K10 gene was amplified by rapid amplification of eDNA ends method (RACE). The gene expressions in the retina of rds, rd and C3B mice were analyzed, respectively by gene-specific real-time quantitative PCR.MAIN OUTCOME MEASURES: The expression contents and patterns of 1200009K10 gene in retina of three kinds of mice.RESULTS: One clone was obtained after RACE of retinal RNA extracted from retina of mice, which had an almost identical sequence with the 1200009K10 gene. Expression of 1200009K10 gene was classified into 6stages: 7, 12, 25, 37, 50 (near sex maturity) and 150 days after born. The expressions of 1200009K10 gene in the retina of rds, rd and C3B mice were low at postnatal day 7 (P7), higher between P7-12, stable between P12-50, and finally increased a little. The expression trend and level of 1200009K10 gene of C3B mice was nearly the same with that of the rds and rd mice, and higher than that of rds and rd mice at P12. Although there were differences in expression levels of 1200009K10 gene among the three kinds of mice, the expression patterns were almost the same.CONCLUSION: 1200009K10 gene may not participate in the development of retinal degeneration.

PurposeTo determine the correlation between expression of MHC classⅠon ovarian tumor cells and T-cell infiltration in vivo on ovarian tumor micro-environment.MethodsThirty three samples of primary epithelial ovarian carcers were analysed with flow cytometry for MHC class Ⅰ expression. Immunohistochemical analysis was performed using monoclonal antibodies that recognize leukocyte differentiation antigens ( LCA+ , CD3 + ) on cryostat sections of primary epithelial ovarian carcinoma. The results were compared with the expression of MHC class Ⅰ expression. ResultsClose correlation were revealed bewteen the expression of MHC class Ⅰ molecules and the numbers of infiltrating LCA+ positive cells ( r = 0. 846, P = 0. 000 1) and CD3 + cells ( r = 0. 738, P = 0. 000 1 ).ConclusionsExpression of MHC class Ⅰ molecules may influence the status of human immunosurveillance in tumor micro-environment on ovarian carcinoma and the assay may be helpful in selection patients for adoptive immnotherapy.

Salidroside antagonizes hypoxia,H2O2,hyper-glucose,homocysteine,smoking,orweightlessness-induced endothelial cell injury,and antagonizes the proliferation of vascular smooth muscle cells and vascular adventitial fibroblasts induced by hypoxia,hyper-glucose,noradrenaline,platelet-derived growth factor,or angiotensin Ⅱ,thus produces vascular protection and improves vascular function.Salidroside plays a dual role of vasoconstriction and vasodilation in regulating resistant blood vessel.The vasodilatory effect of salidroside is endothelium-dependent and endothelium-independent.Salidroside antagonizes vascular contraction and injury induced by KCl,CaCl2,noradrenaline,phenylephrine,homocysteine,hyper-glucose,plateau hypoxia,H2O2,and chlorine.Salidroside relieves cerebral vasospasm induced by subarachnoid hemorrhage,and pulmonary hypertension and pulmonary arterial remodeling induced by hypoxia or monocrotaline in rat,attenuates several experimental atherosclerosis,and enhances plaque stability.

Salidroside antagonizes hypoxia,H2O2,hyper-glucose,homocysteine,smoking,orweightlessness-induced endothelial cell injury,and antagonizes the proliferation of vascular smooth muscle cells and vascular adventitial fibroblasts induced by hypoxia,hyper-glucose,noradrenaline,platelet-derived growth factor,or angiotensin Ⅱ,thus produces vascular protection and improves vascular function.Salidroside plays a dual role of vasoconstriction and vasodilation in regulating resistant blood vessel.The vasodilatory effect of salidroside is endothelium-dependent and endothelium-independent.Salidroside antagonizes vascular contraction and injury induced by KC1,CaC12,noradrenaline,phenylephrine,homocysteine,hyper-glucose,plateau hypoxia,H2O2,and chlorine.Salidroside relieves cerebral vasospasm induced by subarachnoid hemorrhage,and pulmonary hypertension and pulmonary arterial remodeling induced by hypoxia or monocrotaline in rat,attenuates several experimental atherosclerosis,and enhances plaque stability.

Objective To explore the effect of cognitive intervention based on conception of knowledge and belief on cognition level and negative moods in the lung cancer patients undergoing radiotherapy. Methods Toally 84 lung cancer patients hospitalized in our hospital from March 2014 to November 2015 were equally divided into the study group and the control group by a random digit table. The control group received the traditional model of nursing, while the study group was treated with cognition intervention based on conception of knowledge and belief as well as the same routine care as in the control group for 4 weeks. Before and after 4 weeks intervention, the two groups were compared in terms of their knowledge, belief, behaviors, self care ability, depression and anxiety. Results Before intervention, there were no significant differences between the two groups in terms of their knowledge, belief, behaviors, self care ability, depression and anxiety (all P>0.05). After the intervention, the scores on knowledge, belief, behavior and self care ability in the study group were all significantly higher than those of the control group and the scores on anxiety and depression were both statistically significantly lower than those of the control group (all P<0.05). Conclusion The cognition intervention based on conception of knowledge and belief on cognition level and negative moods to the lung cancer patients is effective in improvement of their knowledge, belief, behavior as well as in alleviation of their anxiety and depression.