Objective To study and observe the changes of pancreatic blood flow and blood miR indexes of patients with pancreatic cancer.Methods 70 patients with pancreatic cancer were selected as the observation group,70 health persons of the same age at the same period were selected as the control group, then the blood flow indexes and blood miR of observation group’s lesion and tumor adjacent tissue and control group’s healthy pancreas were detected,and the detection results of control group and observation group were compared,then the above indexes of observation group with different Hemrick stages were compared.Results The lesion blood flow indexes of observation group were all obviously worse than those of control group(P<0.05),blood miR indexes were all higher than those of control group(P <0.05),the lesion and tumor adjacent tissue blood flow indexes all had obvious differences(P<0.05),and the above indexes of observation group with different Hemrick stages all had significant differences(P<0.05).Conclusion The pancreatic blood flow and blood miR of patients with pancreatic cancer all show obvious abnormal state,and the differences of indexes of patients with different Hemrick stages are all prominent, so the pancreatic blood flow and blood miR indexes all have higher value for the patients with pancreatic cancer.

Objective To explore the common influencing factors on the severity of Reinke's edema of the vo-cal cord and to analyze the factors of recurrence.Methods To analyze the relationships among the degrees and smoking,voice overuse,and laryngopharyngeal reflux for 85 patients with Reinke's edema of the vocal cord,and an-alyze the risk factors of recurrence.ResuIts 84.7% of the patients were male with the mean age of 51.35 years. There were statistical differences among smoking history of more than 25 years (P<0.001),laryngopharyngeal re-flux (P<0.01),and voice overuse (P<0.01).Follow ups lasted 1 year and 7 patients showed recurrence.ConcIu-sion Reinke's edema was related to long - term smoking and common in senior male patients.The degree of Reinke's edema was positive correlation with smoking,laryngopharyngeal reflux and voice overuse.The most impor-tant factor is smoking history of more than 25 years.The longer smoking history,the more severe Reinke's edema.

Objective In order to carry out the research of the non-invasive core temperature measurement,the paper designs a portable sixteen channels temperature measurement system. Method Using MCU MSP430F149,this paper applies the technology of analog signal acquisition and digital signal process to establish a multi-channel temperature system. Result The system can store and send the temperature signals through wire and wireless communication mode. Conclusion The system is stable and reliable,and the precision of the system satisfies the clinical demand.

Objective To explore a reliable method to establish a rat model of hyperuricemia associated with abnormal uric acid excretion, and to lay the foundation for the study of pathogenesis of uric acid excretion disorder and the optimization of the treatment plan.Methods The models were established respectively by potasium oxonate(300 mg/kg) with pyrazinamide (300 mg/kg) or ethambutol(250 mg/kg).Continuous dosing for 1, 3 and 5 weeks, to determine the content of uric acid in rat blood, urine, and stool, the function of liver and kidney was detected and pathological examination was performed.Results The blood uric acid in the potasium oxonate and ethambutol group was increased first and then decreased, while in the potasium oxonate and pyrazinamide group were increased steadily and the excretion of uric acid in urine was stable during the continuous administration.The two methods showed no harmful effect on the liver and kidney function.Conclusions A stable rat model of hyperuricemia associated with uric acid excretion disorder can be effectively established by potassium oxonate and pyrazinamide, exhibiting similar manifestations of clinical hyperuricemia and uric acid excretion disorder.

Objective To determine CD+4CDnull28 T cells and their relationship with the microinflammatory state and left ventricular hypertrophy in end-stage renal disease (ESRD) patients.Methods One hundred and twenty ESRD patients were selected,including sixty non-dialytic stage 5 chronic kidney disease patients(CKD5 stage) and thirty maintenance dialysis patients(CKD5D stage).In addition,thirty healthy adults from the hospital medical center were selected as the control group.Flow cytometry was used to detect the frequencies of T lymphocyte subsets in peripheral blood.All enrolled subjects underwent Doppler echocardiography investigations.Results(1)Compared with the control group,the high sensitivity C-reactive protein and the frequency of CD+4CDnull28 T cells were significantly expanded in CKD5 and CKD5D paients (F=14.887,9.659,all P<0.05).(2)In ninety ESRD patients,the incidence of LVH increased in CKD5 and CKD5D patients (76.67% vs.86.67%).(3)Linear correlation in CKD5 patients showed that:LVMI was positively correlated to duration of hypertension,SBP,P3+,lg(CRP+1),lgiPTH,lg(CD+4CDnull28+1) (r=0.332,0.291,0.269,0.310,0.304,0.396,all P<0.05),as well as negatively correlated to Hb(r=-0.285,P<0.05).Multiple linear regression analysis showed that LVMI was independently associated with lg(CD+4CDnull28+1) and duration of hypertension(F=6.589,P<0.000).ConclusionThere is microinflammatory state in the ESRD patients regardless of whether or not they have dialysis treatment,and they have the high incidence of LVH and exhibit a substantially increased frequency of circulating CD+4CDnull28 T cells.The frequency of CD+4CDnull28 T cells and duration of hypertension are the independent predictors of LVH in non-dialytic stage 5 CKD patients,indicates that these cells may play a critical role in the development of cardiovascular disease.

Objective:To compare the regulatory effect of EF and two Kidney-tonifying recipes as well as Spleen-invigorating recipe on expression of T lymphocytes apoptosis related genes in Corticosterone-treated rats.Methods:To quantify and compare the T lymphocytes apoptosis percentage and the expression the pro-apoptotic and anti-apoptotic genes related to T cell apoptosis in every experimental groups using TUNEL method and flow cytometry assay, as well as the fluorescence real time PCR technology.Results:The percentage of T cell apoptosis and the expression of apoptosis related genes were significantly regulated by EF and two Kidney-tonifying recipes, while the Spleen-invigorating recipe had no significant effect with respect to these parameters.Conclusion:EF can be used to represent the effect of Kidney-tonifying recipes in antagonizing the inhibiting effect of exo-corticosterone with respect to T lymphocytes.

Objective To explore the issues of everyday expenses list used in the cadre wards and find out countermeasures for the purpose of reducing medical disputes and improving the patients’satisfaction.Method Seventy-four patients admitted in our department from July to September 2012 were set as control group,where an investigation was conducted to find out the issues in the practical use of every expenses list and corresponding measures were worked out and used in observation group,which included 78 patients admitted in the department from October to December 2012.The two groups were compared in terms of self-paid articles, re-recording of missed expenses,failure to timely refunding,charging by other means,disagreement on curative nursing items and unclear items for charging.Result The observation group was significantly better than the control in self-paid articles,re-recording of missed expenses and failure to timely refunding(all P<0.05).Conclusions There are some issues in the use of everyday expenses list in the cadre wards.We should take corresponding countermeasures to ensure the interests of the hospital and the patients and the enhanced degree of patients’comfort.

ObjectiveTo evaluate Fuji intelligent color enhancement (FICE) chromoendoscopy for diagnosis of early gastric cancer. MethodsFrom February 2010 to March 2011 ,a total of 67 patients with suspected gastric mucosal lesions were enrolled in this study. The lesions were observed with magnifying endoscopy, FICE, magnifying chromoendoscopy and indigo-carmine-magnifying-chromoendoscopy.Suspected gastric mucosal pit patterns and microvascular morphology were compared. Targeted biopsy was performed on suspected locations. Sensitivity, specificity and pathological consistency were compared between the 3 procedures. ResultsOut of 67 patients, 17 were diagnosed as having early gastric cancer.There was no difference among magnifying endoscopy, FICE magnifying chromoendoscopy and indigo-carmine-magnifying-chromoendoscopy in pit pattern findings,however, FICE magnifying chromoendoscopy was superior to other 2 procedures in displaying capillary loop. The sensibility, specificity and pathological accordance rate of FICE magnifying chromoendoscopy were 94. 1％ ( 16/17 ), 98. 0％ (49/50) and 97.0％(65/67), which were higher than those of the magnifying endoscopy [58.8％ ( 10/17), 84％ (42/50)and 77.6％ ( 52/67 )] ( P ＜ 0. 05 ), and were not different from those of indigo-carmine-magnifying-chromoendoscopy [88.2％ (15/17), 96％ (48/50) and 94.0％ (63/67)] (P ＞0.05). Conclusion FICE magnifying chromoendoscopy provides rather clear images of gastric mucosa and intrapapillary capillary, thereby improving the accuracy of endoscopic biopsy and then imporving the diagnosis rate of early gastric cancer.