Objective:To observe oncosis in human peripheral blood T lymphocytes.Methods:Peripheral blood T lymphocytes of healthy adult was separated with Percoll(1.073 g/ml)and harvested by using nylon column.The cultured cells were divided into control and dexamethasone(DXM)group,and cell morphology was observed through light microscope,electron microscope and fluorescent microscope.And incidence rate of oncosis was analyzed by flow cytometry.Results:①Oncosis could be observed in cultured T lymphocyte after 96h.②In different concentrations of DXM group(1?10-6,1?10-5,1?10-4,1?10-3mol/L),The incidence of oncosis T lymphocytes was(3.49?0.42)%,(5.17?0.48)%,(8.44?0.72)%,(17.93?1.50)%.③During different cultured period(48,72,96,120h),The oncositic rate of T lymphocytes in DXM group was(0.53?0.10)%,(6.36?0.80)%,(20.60?1.59)%,(25.56?1.76)%.Conclusion:Oncosis can be seen in human peripheral blood T lymphocytes,and DXM could induce oncosis.

Acute kidney injury(AKI) has emerged as a major public health problem that leads to decreased survival.In AKI,cell hyperplasia,hypertrophy and apoptosis are related to the cell cycle control.Two supervisory restriction points,G1/S and G2/M checkpoints,are responsible for cell-cycle control.Furthermore,cell cycle regulatory proteins are also involved in the regulation of the cell cycle in AKI.This review focuses on the cell cycle regulation mechanism of AKI.

Objective: To deeply investigate the influential factors of mental health in medical students by structural equation modeling.Methods: 480 medical students were tested with SCL-90,adolescent self-rating life events check list and simplified coping style questionnaire,then theoretical model were established and confirmed for the relationships of life events,satisfaction degree with their profession,coping styles and mental health.Results: Study stress,human relations,satisfaction degree towards their profession and coping styles closely correlated with mental health status,and the structural equation modeling fitted the data very well,GFI and AGFI were both larger than 0.90.Conclusion: Use of structural equation modeling has more advantages than the traditional statistical methods,but it should combine with professional knowledge when it is used.

Glucokinase (GK) is considered to be the key regulatory enzyme in glucose metabolism, whose activity changing will lead to glucose metabolic disturbance and diabetes. Recently, people have realized that there is a good extensive perspective in diabetes research by means of glucokinase investigation. In this paper, we review the mechanism of diabetes resulting from abnormality of glucokinase activity and the status of glucokinase activators, propose the viewpoint that glucokinase is a powerful target for diabetes therapy and research, and illuminate that searching of glucokinase activators is the guidance in the future.

Objectives:To observe the clinical features,related factors,prevention and treauments of complications on early enteral nutrition(EN) in severe head injured patients.Methods:360 patients with severe head injuries were enrolled in this study.GCS score was used to assess the patient's situation.After nasogastric(jejunal) tube was inserted,enteral nutrition was started from 48 hours after injury.The maximal EN volume(mL/d) in the first week after injury,time used for reaching to TEN(d),number of patients that achieved TEN in the first week following injury,blood glucose,serum albumin and ALT were observed.The complications due to enteral nutrition were analyzed. Results:Features of complications with early EN were that the abdominal distension,vomiting and aspiration were found in the first week and diarrhea occurred in the following weeks.A positive correlation between abdominal distension,vomiting and nasogastric tube was noted,while diarrhea had a negative correlation to GCS score and albumin level. Conclusions:The complications related to early enteral nutrition after severe head trauma correlated inversely to the degree of injury and serum albumin level.Utilization of nasojejunal tube could decrease the incidences of abdominal distension and vomiting.

New healthcare reform has been achieved remarkable effects and had a lot of acceptance by people at home and abroad. As the reason of the historical and medical system problem, high Medicine Price still exists in the most area of China. It is necessary to establish a reasonable mechanism of drugpricing and improve financial efficiencies of medical reform to guarantee the smooth and healthy development of healthcare reform.

Acute kidney injury (AKI) has emerged as a major public health problem that leads to decreased survival. Renal tubular epithelial cells are mainly damaged cells in AKI. Renal tubular epithelial cell remodeling plays a vital role in AKI. The regulatory mechanism of renal tubule remodeling is not yet clear. This review is written to address the renal tubule remodeling regulation mechanism of AKI.

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Objective To investigate the phacoemulsification combined with trabeculectomy in glaucoma complicated with cataract treatment clinical curative effect.Methods 72 cases of glaucoma patients with cataract were randomly divided into the combined group and control group with 36 cases in each group,the combined group using phacoemulsification and trabeculectomy in the treatment,while the control group was treated with trabeculecto-my,combined group and control differences in visual acuity,intraocular pressure,anterior chamber depth and postoper-ative complications of patients were compared.Results After treatment,the two groups of intraocular pressure ( list the concrete data ) , anterior chamber depth ( list the concrete data ) were ( list the concrete data ) significantly improved (t=2.98,3.06,15.21,5.17,all P<0.05).In addition,the intraocular pressure after the operation of the combination group (10.65 ±1.58) mmHg,anterior chamber depth (2.41 ±0.81) mm compared with the control group after obvious difference (t=4.98,3.22,all P<0.05).In 1 weeks after operation,1 months and 3 months com-bined group's vision is significantly superior to the control group (t=2.87,3.38,4.01,all P<0.05).The incidence of complications of operation united group ( 11.11%) was significantly lower than the control group ( 25.00%) (χ2 =3.98,P<0.05).Conclusion Compared with the traditional method,phacoemulsification and trabeculectomy for glaucoma and cataract treatment has obvious curative effect,low incidence of adverse reactions,postoperative IOP control satisfaction,faster visual recovery characteristics,deserves generalization and application.

Long non-coding RNAs (lncRNAs) have gradually become a research hotspot in recent years. The abnormal expression of some lncRNAs has been proven to promote tumorigenesis and progression. Among the lncRNAs, metastasis-associated lung adenocar-cinoma transcript 1 (MALAT-1) has attracted considerable attention in various tumor studies, particularly lung cancer. Therefore, the mechanism in which MALAT-1 regulates the cellular activities of tumors should be analyzed. This review mainly discusses the molecu-lar structure, biological function, and role of MALAT-1 in regulating cell proliferation, tumor metastasis, cell cycle, apoptosis, and so on. Furthermore, the role of MALAT-1 in epigenetic regulation is appropriately illustrated to provide a novel perspective in literature on the prevention and treatment of tumors.