Objective To study the antitumor activity of extract from Salvia plebeia and investigate whether the extract induce apoptosis of K562 cells. Methods The aqueous, petroleum ether, dichloromethane (CH2Cl2), ethyl acetate, and butanol extracts were prepared from the aerial parts of 5. plebeia. Taking fluorouracil as reference, the cytotoxic activities of these extracts on HeLa, A549, SGC-7901, HCT-116, K562, LoVo, DU-145, and HepG2 cells were evaluated. To clarify the apoptosis of K562 cells induced by CH2Cl2 extract, the methods of Hoechst 33258 staining, flow cytometry assay, and DNA ladder assay were investigated. Results The CH2Cl2 extract showed the most potent cytotoxic effect against K562 cells, with an IC50 < 15 μg/mL for 3 d treatment. The characteristic apoptotic symptoms such as DNA fragmentation and chromatin condensation were also observed in the K562 cells. Conclusion The CH2Cl2 extract from S. plebeia may inhibit the cancer cell proliferation by inducing cell apoptosis.

Objective To establish an objective health literacy evaluation index system of dementia care for scientific assessment.Methods Literature reviews,in-depth interviews,participatory rapid appraisal,and focus group discussion were adopted to developed a scale and checked its items.Then the scale was utilized to investigate 280 residents,and its reliability and validity were examined.Results The health literacy evaluation index system of dementia care included 56 items which belong to four dimensions of knowledge,attitude,behavior and skills.The total Cronbach α was 0.818,split-half reliability was 0.881,the correlation between the total score and each dimension were significant (r =0.417-0.747).KMO statistic in factor analysis was 0.739 and 8 factors predicted 46.305％ of total variance.What's more,the correlation between each items score and dimension were significant (r =0.221-0.717).Conclusions The health literacy scale presents acceptable reliability and validity in the surveyed sample,which can be used as a valid measure of health literacy in community health service centers and as the basis of health education.

Objective? To explore the status quo of nurses' dementia care-related literacy, caring behavior and career identity, to discuss the effects of caring behavior and career identity on dementia care-related health literacy, and to provide a basis for nursing management and professional training. Methods? In this cross-sectional study, totally 650 nurses from 10 Class Ⅲ Grade A hospitals in Beijing who had provided care in departments of geriatrics or neurology were selected by convenient sampling between April and November 2017, and investigated with the General Information Questionnaire, Caring Dimensions Inventory(CDI), Career Identity Rating Scale and Dementia Care-related Health Literacy Scale. Totally 650 questionnaires were distributed, and 602 valid questionnaires were collected, with a recovery rate of 92.6%. Results? The 602 nurses' total scores of dementia care-related health literacy, caring behavior and career identity were (74.79±7.96), (88.87±5.02) and (84.35±9.14), respectively. Correlation analysis showed that nurses' caring behavior and career were positively correlated with dementia care-related health literacy, respectively (P＜ 0.01). Hierarchical regression analysis showed that general information, caring behavior and career identity accounted for 74.5% of total variances of dementia care-related health literacy in which caring behavior could account for 33.2% and career identity 34.2%. Conclusions? Nurses' career identity and caring behavior were supplementary to their dementia care-related health literacy, which can enhance their career identity, standardize their caring behavior and improve their dementia care-related health literacy, thus providing better care services for patients with dementia.

ObjectiveTo construct a comprehensive and simple risk assessment model for Alzheimer's disease (AD), which is suitable for the promotion of the elderly population, to predict the future risk of AD among the elderly and to intervene in the early stage. MethodsIn June 2018, risk factors were screened by literature review and brainstorming, and then combined with Meta-analysis reappraisal and structured expert consultation to screen risk factors and OR values into the model. Individual relative risk and absolute risk in the next five years were calculated according to the Harvard Cancer Index formula. ResultsThe model finally included 24 indicators: female, no spouse, education level, personality, family history of dementia, herpes virus infection, middle-aged hypertension, diabetes, stroke, brain injury, hearing impairment, periodontitis, depression, smoking, drinking alcohol, coffee/tea, deep-sea fish, vegetables and fruits, exercise, sleep, social life, waist circumference, serum homocysteine and serum thyroxine. ConclusionsThe model of individual AD risk established by modified Harvard Cancer Risk Index can predict the relative and absolute risk of Alzheimer's disease in the future, which provides the basis and guidance for screening and intervention of high-risk population.

Objective? To compare the predictive and applicable power of 3 dementia risk assessment tools for Alzheimer's disease in Chinese elders. Methods? In this case-control study, totally 80 elderly patients with Alzheimer's disease hospitalized in 5 hospitals in Beijing from May 2013 to August 2018 were selected by convenient sampling as the case group, while 80 elders with normal cognitive function were randomly selected as the control group from the same region. Clinical data of both groups were collected, and the subjects' morbidity risk of Alzheimer's disease was assessed using Barnes Model, Australian National University Alzheimer's Disease Risk Index (ANU-ADRI) and Chinese Alzheimer's Disease Risk Assessment Model (C-ADRA). The predictive power of different assessment tools was thus compared. Results? There was significant difference in family history of dementia, craniocerebral injury, hearing disorder, depression, lifestyle as well as some physiological and biochemical criteria between case and control groups. The accuracy, sensitivity and specificity of C-ADRA in predicting Alzheimer's disease in Chinese elders was better than that of Barnes and ANU-ADRI models. Conclusions? C-ADRA has a better predictive power for Alzheimer's disease in Chinese elders. Targeted prevention may be provided for patients according to the results of risk assessment, thus promising a guarantee for the prevention and control of Alzheimer's disease in clinical nursing and prevention.

Objective To investigate the prevalence of genes conferring aminoglycoside resistance in multidrug-resistant strains of Acinetobacter baumannii (MDR-ABA).Methods Multidrug-resistant A.baumannii strains were isolated during the period from August to November 2012 from patients in the affiliated hospital of Jiangsu University and the First Hospital of Zhen-jiang.Kirby-Bauer diffusion method was used to determine the susceptibility of these strains to antimicrobial agents.PCR was performed to detect the aminoglycoside resistance genes.Results The 36 MDR-ABA strains showed high resistance rates to most antimicrobial agents except cefoperazone-sulbactam.The prevalence of the genes conferring aminoglycoside resistance, aac (3)-I,aac (6’)-Ib,aph (3’)-I and armA,was 72.2% (26/36),72.2% (26/36),80.6% (29/36)and 80.6% (29/36), respectively.Conclusions The MDR-ABA strains in this study are highly resistant to antimicrobial agents,which is closely as-sociated with the genes conferring aminoglycoside resistance.

Objective To explore the efficacy of nystatin vaginal effervescent tablets , nifuratel tablet combined with recombinant human interferon alpha 2b suppository in the treatment of candida vaginitis .Methods 124 patients with candida vaginitis were selected as the research subjects .The patients were randomly divided into two groups according to different treatment .The control group was treated with nystatin vaginal effervescent tablets +nifuratel tablet,the study group was given recombinant human interferon alpha 2b suppository on the basis of the control group.The symptoms,clinical curative effect,quality of life score,adverse reaction rate were observed and compared in the two groups .Results There were no statistically significant differences between the two groups in symptom and physical signs before treatment (all P>0.05).After treatment,the vulvar itching,burning pain,abnormal leucorrhea,redness symptoms and signs of the study group were (0.95 ±0.44)points,(1.02 ±0.51)points,(1.13 ± 0.62) points,(1.09 ±0.56) points,which were significantly lower than those of the control group [(1.42 ± 0.98)points,(1.28 ±1.02)points,(1.38 ±1.17)points,(1.37 ±1.12)points,t=2.529,2.512,2.505,2.618,all P<0.05].The total effective rate of the study group was 95.2％,which was significantly higher than 74.2％ of the control group (Z=-2.839,P<0.05).After treatment,the quality of life scores of physiological function ,bodily pain,mental health,emotional function and social function in the study group were (80.16 ±9.23)points,(84.22 ± 9.95)points,(87.49 ±10.46) points,(89.27 ±12.35) points,(88.21 ±11.74) points,which were significantly higher than those in the control group[(67.58 ±5.34)points,(71.49 ±7.28)points,(73.52 ±7.61)points,(76.83 ±8.32)points,(75.51 ±8.16) points,t=9.728,8.577,9.036,7.039,7.537,all P<0.05].The incidence rate of adverse reactions of the study group was significantly lower than that of the control group (χ2 =10.978,P<0.05). Conclusion Nystatin vaginal effervescent tablets ,nifuratel tablet combined with recombinant human interferon alpha 2b suppository in the treatment of candida vaginitis can significantly improve clinical symptoms ,improve the therapeutic effect,and improve the quality of life of patients with significant clinical value ,it is worthy of reference .

Objective To explore the clinical effect of ethinyl estradiol cyproterone tablets ( Diane -35 ) combined with ovulation induction in the treatment of polycystic ovary syndrome infertility .Methods 70 patients with polycystic ovary syndrome were enrolled , and they were randomly divided into two groups according to the random number table of the computer ,35 cases in each group .The control group was treated with Diane -35,the observation group was given ovulation induction .The clinical parameters ,vaginal ultrasound results ,cycle ovulation rate ,pregnancy rate,adverse reaction rate and quality of life were compared between the two groups .Results There were no statistically significant differences in uterine volume , intima thickness , bilateral ovarian volume and bilateral follicle number between the two groups after treatment (t=0.055,0.308,0.991,0.435,0.473,0.248,all P>0.05).The cycle ovulation rate of the observation group was significantly higher than that of the control group (80.88％ vs. 54.05％,χ2 =8.451,P<0.01).The shape,color,thought and energy score of the observation group were significantly higher than those of the control group (t=4.819,3.519,4.735,6.804,all P<0.01).The pregnancy rate of the observation group was significantly higher than that of the control group (25.71％vs.5.71％,χ2 =5.285,P<0.05). Conclusion Diane-35 combined with ovulation induction in the treatment of polycystic ovary syndrome infertility has significant clinical efficacy .

Objective: To identify the stakeholders of prevention and control of obesity among middle school students and analyze their motivations and interest demands for participation, in order to provide constructive recommendations for facilitating their effective engagement of stakeholders in prevention and control of obesity among middle school students. Methods: Stakeholders and their types were identified through a combination of literature analysis and consultation with 17 experts in children and adolescents for prevention and control of obesity. From December 2023 to January 2024, by applying convenience sampling, face to face semi structured interviews were conducted with 11 individuals engaged in prevention and control of obesity for middle school students and focus group interviews were conducted with 48 students and parents. Thematic analysis was employed to obtain motivations and interest demands for stakeholder involvement in prevention and control of obesity. Results: A total of 29 subcategories within 7 major categories of stakeholders were identified, including government and relevant functional departments, non profit organizations and communities, forprofit organizations and individuals, schools, professional and technical personnel, families and individuals, and scientific research institutions, with 28 subcategories being confirmed stakeholders. Among these stakeholders, there were 3 types of cooperative relationships:management, cooperation, and service. There were some factors hindering cooperation among stakeholders including imperfections in specialized policies and lack of long term cooperative mechanisms, flaws and interest conflicts in the evaluation system, insufficiency of material resources, and poor health values. In response to these challenges, interviewees proposed to convene a working group for the prevention and control of adolescent obesity. They also suggested making a contract of responsibility, strengthening supervision over for profit organizations, enhancing advocating efforts for prevention and control of obesity, and increasing financial support. Conclusions The prevention and control of obesity for middle school students can be facilitated by forming a collaborative alliance based on the roles and relationships of stakeholders identified in the present study. Perceived conflicts of interest among stakeholders can be reconciled by employing strategies of interest integration, goal alignment, and spiritual integration, to enhance the feasibility, participation and sustainability of obesity intervention.

Objective? To review the meta-analysis of lifestyle risk factors for Alzheimer disease in order to find out the quality of the current articles and to provide evidence for preventing Alzheimer disease. Methods? CNKI,Wanfang,CBM,PubMed、EMBASE、ProQuest、Cochrane Library were searched on computer for Meta-analyses in lifestyle risk factors of Alzheimer disease. Two authors independently selected the articles,extracted data,and used JBI to evaluate the quality of literature system evaluation and the evaluation method of meta-analysis evidence grade for methodological and evidence quality evaluation. Results? A total of 34 articles were included which involved smoking,alcohol consumption,exercise,social activities,sleep, diet,coffee/tea. Among them, the effects of smoking and drinking alcohol and coffee/tea need further support by evidence. Conclusions? Intervention on lifestyle can be effective in preventing Alzheimer disease,however, more high quality original studies are needed to explore more risk factors of Alzheimer disease.