1.The comparative study of MR diffusion-weighted imaging and MR perfusion-weighted imaging in diagnosing soft tissue tumors
Shaowu WANG ; Lina ZHANG ; Meiyu SUN ; Feige JIA ; Qingwei SONG
Chinese Journal of Radiology 2009;43(2):136-140
Objective To evaluate MR diffusion-weighted imaging (DWI)and MR perfusion-weighted imaging(PWI) in differentiating benign from malignant soft tissue tumors by comparing the related parameters. Methods Fifty patients with soft tissue tumors verified by pathology( benign 24, malignant 26) underwent DWI and dynamic contrast-enhanced T2 * -weighted PWI. DWI and PWI data of benign and malignant soft tissue tumors were acquired at the workstation and their difference was analyzed with t-test. The diagnostic accordance rate was verified with x2-test. Subjective overall performance of two techniques were evaluated with receiver operating characteristic (ROC) analysis. Results ADC values of benign and malignant tumors were (2. 03±0. 36) × 10-3 mm2/s, ( 1.52±0. 39) × 10-3 mm2/s,respectively. The signal intensity decrease of them during the first-pass perfusion (SIdecrease ) were ( 13.54 ± 3.37 )%, (47. 57 ± 5. 21 ) % ,respectively. The maximum linearity slope rate of TIC ( SSmax ) of them were ( 5.51 ± 2. 54 ) %, (7.94 ± 3. 33) %, respectively. There were significant differences between benign and malignant tumors of ADC value and SIdecrease ( t = 2. 515,2. 938 ;P < 0. 05 ), while there was no significant difference in SSmax (t = 1. 272,P >0. 05). When the threshold of ADC value was 1. 866 × mm2/s, sensitivity and specificity for determining malignant tumors were 84. 6% (22/26)and 83.3% (20/24). When the threshold of SIdecrease was 40. 33% ,sensitivity and specificity for determining malignant tumors were 88. 5% (23/26)and 75.0% (18/24). In type Ⅰa of TIC,the proportion of benign soft tissue tumor was 3/24 and malignant tumor was 20/26. In type Ⅰb , benign tumor was 14/24 and malignant tumor was 3/26. In type Ⅰc, malignant tumor was 3/26. In type Ⅱ ,benign tumor was 7/24. The diagnostic accordance rate of DWI and PWI were 84. 0% (42/50) and 82. 0% (41/50), respectively. There was no significant difference between them ( x2 = 0. 8, P >0. 05). The accuracies of them were 81.7% , 83. 6% respectively by the area under the ROC curve (AUC). The sensitivity of PWI in diagnosing malignant soft tissue tumors was higher. Conclusions ADC value and SIdecrease are Valllable diagnostic parameters in differentiating benign and malignant soft tissue tumors. The threshold of these parameters for diagnosing malignant soft tissue tumors are 1. 866 × 10-3 mm2/s and 40. 33%, respectively. The type of TIC can help to distinguish malignant tumors from benign tumors, while the SSmax can not. The accuracies of DWI and PWI in the diagnosis of malignant soft tissue tumors are moderate. Compared with DWI, PWI should be selected firstly because of its higher sensitivity in diagnosing malignant tumors.
2.Values of kinetic features measured by computer-aided diagnosis for breast MRI
Lina ZHANG ; Zuowei ZHAO ; Qingwei SONG ; Shaowu WANG ; Yanwei MIAO
Chinese Journal of Radiology 2012;(11):998-1001
Objective To investigate the value of kinetic features measured by computer-aided diagnosis (CAD)for breast MRI.Methods One hundred and sixty four lesions diagnosed pathologically by operation or biopsy comprised the analysis set.Automated lesion kinetic information from CADStream programs for breast MRI was identified.Three CAD variables were compared for benign and malignant lesions: initial phase peak enhancement (greatest percentage of signal intensity increase on first contrast enhanced sequence),delayed phase enhancement categorized by a single type of kinetics comprising the largest percentage of enhancement (washout,plateau,or persistent),and delayed phase enhancement categorized by single most suspicious type of kinetics (any washout > any plateau > any persistent).Morphological characteristics of breast lesions were described according to breast imaging and reporting data system (BI-RADS).Initial phase peak enhancement mean values between benign and malignant breast lesions were compared by using Wilcoxon rank-sum test,delayed phase enhancement categorized by a single type of kinetics comprising the largest percentage of enhancement or by single most suspicious type of kinetics between benign and malignant breast lesions were compared by using Chi-square test.Results There were 72 benign and 92 malignant breast lesions.A total of 123 (75.0%) mass lesions were identified,and the other 41 (25.0%) lesions showed no mass.Thirty lesions were BI-RADS-MRI 2,68 lesions were BI-RADS-MRI 3,43 lesions were BI-RADS-MRI 4,23 lesions were BI-RADS-MRI 5.Initial phase peak enhancement mean values of benign and malignant lesions were 237% (69% to 629%)and 336% (86% to 793%),respectively.There was no significant difference between benign and malignant lesions in initial peak enhancement mean value (Z =-1.626,P =0.104).Delayed phase enhancement categorized by single most suspicious type of kinetics (any washout > any plateau > any persistent) for benign and malignant lesions were 15,10,47 and 2,3,87 respectively.There was a significant difference between benign and malignant lesions (x2 =23.562,P =0.000).Initial peak enhancement value < 100% or ≥100% were 5 and 67 for benign lesions,3 and 89 for malignant lesions,respectively.There was no significant difference between benign and malignant lesions at 100% threshold (x2 =1.181,P =0.277).Delayed phase enhancement categorized by a single type of kinetics comprising the largest percentage of enhancement (washout,plateau,or persistent) for benign and malignant lesions were 48,6,18 and 47,15,30 respectively.There was no significant difference between benign and malignant lesions (x2 =4.496,P =0.106).Conclusions Of CAD kinetics analyzed,only delayed enhancement categorized by most suspicious type is helpful for the differentiation between benign and malignant lesions.However,there is significant overlap between initial peak enhancement at 100% threshold or delayed kinetics categorized by largest percentage enhancement types of benign and malignant lesions,so lesion morphologic features should be considered.
3.The comparative study of MR perfusion-weighted imaging and 1 H-MR spectroscopy in diagnosing soft tissue tumors
Lina ZHANG ; Shaowu WANG ; Qingwei SONG ; Meiyu SUN
Chinese Journal of Radiology 2008;42(12):1298-1302
Objective To evaluate multiple magnetic resonance (MR) imaging techniques in the differentiation of benign and malignant soft tissue tumors by comparing different information from MR perfusion-weighted Imaging (MR-PWI) and 1 HMR spectroscopy (1 H-MRS).Methods Forty patients with soft tissue tumors underwent conventional MR imaging,dynamic contrast-enhanced T2*-weighted MR-PWI and 1 H proton MR spectroscopy.The differences of perfusion and 1 H-MRS parameters of benign and malignant tumors were analyzed with t test.Results There was significant difference between benign and malignant tumoral tissues of BF value and Cho/Cr ratio(t=2.531,2.927,P < 0.05),while BV,MTT,Cho,Cr or Lip peak value were not.TIC was different between benign group (Ib) and malignant group (Ia).When the threshold value of BF was 4.35 ml ·100 mg-1·min-1,sensitivity and specificity for determining malignant tumors were 81.8%(18/22),72.2%(13/18),respectively.When the threshold value of Cho/Cr ratio was 3.22,Sensitivity and specificity for determining malignant tumors were 86.4% (19/22),88.9% (16/18),respectively.The abnormal wave crest is detected at 2.0-2.1ppm in 5 malignant tumors (2 malignant schwannoma and 3 malignant fibrous histiocytoma),while the other 35 cases were not.Conclusion The BF value and Cho/Cr ratio were both valuable diagnostic parameters in differentiating benign and malignant soft tissue tumors.TIC was helpful to distinguish malignant tumors from benign tumors,while the sensitivity and specificity of 1 H-MRS in diagnosing malignant soft tissue tumors were both higher.
4.MRI characteristics of solid papillary carcinomas in situ of breast
Li'na ZHANG ; Weisheng ZHANG ; Qingwei SONG ; Ailian LIU ; Shaowu WANG ;
Chinese Journal of Interventional Imaging and Therapy 2017;14(9):539-542
Objective To evaluate MRI characteristics of solid papillary carcinomas (SPCs) in situ of the breast.Methods A retrospective study included 5 patients with pathologically confirmed SPC in situ was performed.MRI data before operation including conventional MRI,dynamic contrast enhanced MRI (DCE-MRI) and DWI were analyzed.Results All the lesions showed iso/hypointensity on T1 FSPGR sequence,iso/hyperintensity on FSE T2WI sequence and STIR sequence.Mass enhancements were observed for all lesions with oval or irregular shapes on DCE-MRI.The margin of lesions were circumscribed,and internal enhancements were homogeneous or heterogeneous.Time intensity curve appeared a rapid increase in initial contrast phases and platform or outflow types in delayed phases.All the lesions on DWI showed slightly hyperintensity with the ADC value range from 1.34 × 10-3 mm2/s to 1.96)× 10-3 mm2/s.Conclusion MRI manifestations of SPC are characteristics,which may provide valuable information to distinguish SPC in situ from other invasive breast carcinomas.
5.Serum Free Fatty Acids Composition, Waist/Hip Ratio and insulin activity in Patients with Essential Hypertension
Shun WANG ; Aiqun MA ; Shaowu SONG ; Qinghai QUAN ; Xiaohui ZHENG ; Xinfeng ZHAO
Chinese Journal of Hypertension 2006;0(08):-
Objective To investigate the relationship of fasting serum FFA composition with central obesity, hypertension, insulin resistance, levels of serum zinc and age, gender-related differences. Methods Serum FFA were determined by high performance liquid chromatogram in 232 commuity|based subjects aged between 35 and 55 years: 109 hypertensives, 123 normotensives. Results Multivariate stepwise linear regression models and correlation analysis showed that serum FFA composition (including levels of n3PUFA, P/S ratio, C20:5/C18:3, C22:6/C20:5 and C20:4/C18:2 ratio) were linearly correlated with waist/ hip ratio, insulin sensitivity index, levels of serum zinc and related to the habit of diet (all P
6.Mechanism Study on Active Ingredients of Danggui Shaoyao San for Treating Alzheimer's Disease Based on Biological Network
Zhenyan SONG ; Yixuan CHEN ; Jingping YU ; Shaowu CHENG
World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(6):834-846
Objective: To study the mechanism of action of Danggui Shaoyao San (DSS) in the treatment of Alzheimer'sdisease (AD) from a systematic network level using network pharmacology. Methods: The active ingredients of DSS intreating AD was screened from the Traditional Chinese Medicine System Pharmacology Database (TCSMP), and thedrugs-targets-disease network was built. Target organ localization network, GO analysis and Pathway enrichmentanalysis were used for bioinformatics analysis. Results: 51 kinds of DSS active ingredients were screened by networkpharmacology, showing 377 predicted targets of active compounds. Among them, 197 targets were associated with AD, and the pharmacodynamic targets of DSS active compounds were highly enriched with AD-related signals. The pathwaywas closely related to the reduction of Aβ aggregation and inhibition of tau hyperphosphorylation and biological processessuch as anti-inflammatory and anti-apoptosis, and the mRNA expression of the targeted gene was mainly enriched in theliver, heart, and brain. Conclusion: DSS active compounds interact with multiple targets in multiple ways in a synergisticmanner, mainly to produce important therapeutic effects on AD with anti-inflammatory, anti-apoptotic, enhancingmetabolism and immune system.
7.Effect of Danggui Shaoyaosan on Mitochondrial Homeostasis and AMPK/SIRT1/PGC-1α Signaling Pathway in Rats Model of Alzheimer's Disease
Miao YANG ; Wenjing YU ; Chunxiang HE ; Yijie JIN ; Ze LI ; Ping LI ; Sisi DENG ; Yaqiao YI ; Shaowu CHENG ; Zhenyan SONG
Chinese Journal of Experimental Traditional Medical Formulae 2023;29(3):9-16
ObjectiveTo investigate the effect of Danggui Shaoyaosan (DSS) on the morphology and function of mitochondria in rats model of Alzheimer's disease (AD) and its possible mechanism. MethodRats model of AD was established by injection of streptozocin (STZ) into bilateral ventricles of SD rats. The 40 rats were randomly divided into sham group, model group, low, medium and high dosages of Danggui Shaoyaosan (12,24,36 g·kg-1·d-1) groups,observed the morphological changes of mitochondria in hippocampus of rats by electron microscopy after 14 days of continuous gavage. In situ end labeling(TUNEL) staining used to detect apoptosis and immunofluorescencereactive used to observe the expression of reactive oxygen species (ROS) and peroxisome proliferators-activated receptor γ coactivator lalpha (PGC-1α),quantitative Real-time polymerase chain reaction(Real-time PCR)detected the mRNA expression of dynamin-related protein 1 (Drp1),mitochondrial fusion protein 2 (MFN2) ,cytochrome C oxidase subunit Ⅳ (COX Ⅳ) and PGC-1α. Western blot detected the protein expression of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK),phosphorylation(p)-AMPK,recombinant Sirtuin 1 (SIRT1) and PGC-1α. ResultCompared with the sham group,the results of model group showed that the damage of mitochondria in hippocampus was more obvious,accelerated the ROS production and apoptosis rate (P<0.01),decreased the mRNA level of MFN2,COX Ⅳ,PGC-1α,increased the mRNA level of Drp1,and descended the protein of p-AMPK/AMPK,SIRT1,PGC-1α (P<0.05,P<0.01).Compared with the model group,the medium and high dose of DSS group notably improved the damage of mitochondria,reduced the production of ROS and apoptosis rate (P<0.01),promoted the mRNA expression of MFN2,COX Ⅳ,PGC-1α,inhibited the mRNA expression of Drp1,and up-regulated the protein of p-AMPK/AMPK,SIRT1,PGC-1α (P<0.01). ResultDSS can significantly ameliorate the mitochondrial homeostasis imbalance in AD rats.