Objective To investigate the effects of nitric oxide synthase inhibitor L-NAME on two tone fear extinction design in rats.Methods In AAA design,the rats received fear conditioning,extinction training and extinction test in the same context.In AAB design,the rats received fear conditioning and extinction training in context A,extinction test in context B.In each task,40 male rats were randomly divided into 4 groups (n =10 per group),and L-NAME(10,20 and 40 mg/kg) or saline was injected intraperitoneally (i.p) 30 min prior to extinction training.Percent freezing was used as an index for fear memory during extinction test phases.Further experiments were used to test state-dependency effects or nonspecific changes of locomotor activity that followed L-NAME injection.Results In AAA design,percent freezing was (27.42 ± 6.52) ％ in saline group,and (30.83 ±7.15) ％,(32.49 ± 8.55) ％,(38.94 ± 11.48) ％ in 10,20,40 mg/kg L-NAME group respectively.There was no significant difference in the level of percent freezing among the four groups (P＞0.05).In AAB design,percent freezing was (30.32 ± 6.15) ％ in saline group,and (32.83 ± 6.64) ％,(39.49 ± 8.74) ％,(49.94 ± 10.83) ％in 10,20,40 mg/kg L-NAME group respectively.Compared with saline,only rats with L-NAME 40 mg/kg showed more levels of freezing (P ＜0.01).In state-dependency effects test,compared with Sal-Sal group ((26.73 ±5.62) ％) which received both saline injections 30 min before extinction training and extinction test,respectively,both NAME-Sal group((48.44 ± 10.46) ％) and NAME-NAME group((61.25 ± 13.24) ％) showed more levels of freezing (P＜0.01,respectively).Conclusion These results show that L-NAME produces a task-dependent impairment of fear extinction,and implies that nitric oxide signaling is involved in memory process of certain extinction tasks.

1:64 positive ratio was 87 5%,and 91 3% in partial pressure of arterial oxygen (PaO 2)

Objective: To explore the clinical value of transvaginal ultrasonography in diagnosis of ruptured ovarian cyst. Methods:Retrospective analysis the 46 cases of ruptured ovarian cyst diagnosis by transvginal ultrasonography in our hospital which confirmed by surgery and pathology. Results: In the among 46 cases, 39 cases were the corpus luteum cyst, 14 cases were the endometriotic cyst, were all confirmed by surgery and pathology, the diagnostic accurate rate of corpus luteum cyst is 92.3%, the rate of endometriotic cyst is 57.1%. Conclusion:Transvaginal ultrasonography is accurate, rapid and convenient, provides the reliable basis for the diagnose and different diagnose acute abdominal disease.

OBJECTIVE: To optimize the extraction technology of thrombolytic components predominantly as corilagin from Phyllanthus urinaria.METHODS: The extraction technology was optimized by orthogonal experiment with solvent amount,extracting time and extracting times as factors,and with the comprehensive score of the yield of extract and corilagin content as indexe.The impurity-removing efficacy by centrifugation,acid/base method or ethanol precipitation was evaluated.RESULTS: The optimal extraction technology was as follows: using 8-fold volume of 30% ethanol as solvent for reflux extraction of 3 times(2 hours/ time).The best impurity-removing efficacy was achieved when 90% ethanol was used for precipitation.CONCLUSION: The extraction technology is simple and stable with high yield of active components,and it provides theoretic basis for the industrial production.

This paper discussed the ethical principles in the medical sci-tech journal editing work, namely, respect, fairness, equality, honesty, and combining with the particularity of medical journals, further discussed these principles during choosing manuscripts, inviting manuscripts, peer review, rejection, and so forth. This pa-per proposed that medical journal editors should adhere to in the work of ethical principles, and constantly improve their ethics quality.

Objective To study the relationship of age and high risk behaviors (HRBs) related to HIV/AIDS among men who have sex with men (MSM) in China. Methods One thousand one hundred and nine anonymous questionnaires were collected from MSM in 1999. Univariate analyses were applied to clarify the relation of the age and HRBs. Results The numbers of accumulative partners and oral intercourse behavior were higher in 35 ~ 44 age group (P

Objective:To analyze the clinical feature and treatment of head-neck malignant fibrous histiocytoma (MFH).Method:A retrospective analysis on the clinical data of 28 cases of MFH were carried out, of which 9 were in the maxilla, 3 in the nasal cavity, 5 in the larynx, 3 in the parotid, 3 in the temporal bone, 1 in the hypothyroid, and 4 in the head and neck region. All of the cases underwent immune histochemistry: 4 cases of surgery alone, 23 cases of surgery followed by radiotherapy, and 1 case of radiotherapy alone.Result:Except 5 cases lost follow-up after 1 year, all the other cases were followed-up over 3 years, the survival rate for 1 or 3 years was respectively 96.4%(27/28) and 57.1%(16/28); 23 cases were followed up for 5 years, the survival rate was 26.1%(6/23). The recurrent rate in 3 years was 60.7%(17/28) ,with 1 to 7 times recurrence at a mean interval of 5.6 months. Twelve recurrent cases were adopted expanded resection of non-defined operation except 1 case with radiotherapy.Conclusion:The diagnosis of MFH depends on the technology of immune histochemistry. Early diagnosis, expanded resection, and integrated therapy could reduce the recurrence and increase the survival rate; the recurrence could adopt expanded resection of non-defined operation to prolong the life.

Objective To explore the causal influences between activated regions in executive control network when participants were performing attention network test (ANT)under passive hyperthermia using Granger causality analysis.Methods Thirty male healthy participants which were randomly divided into control group (n=1 5)and experimental group (n=1 5)performed ANT task during two thermal conditions:hyperthermic condition (50℃)and normothermic condition (20℃)during functional magnetic reso-nance imaging.The data processing was performed using statistical parametric mapping 8 (SPM8).Granger causality analysis was performed on the resulted activated regions,as well as the correlations of the GC values with the reaction time in both groups.Results Between-group comparisons on Granger causality analysis revealed significantly enhanced influences of anterior cingulate gyrus and right inferior parietal lobule on dorsolateral prefrontal cortex,and bidirectional influences between dorsolateral prefrontal cortex and right superior parietal lobule,but decreased influence between right superior parietal lobule and right inferior parietal lobule.The correlation analysis showed significant correlation between GC value of right superior parietal lobule to dorsolateral prefrontal cortex and the reaction time in control group (r=-0.558,P =0.031),but yet there was no significant correlation in experimental group (r=0.355,P =0.257).Conclusion Heat stress affected the causal influences within the executive control network,which subse-quently disrupted the executive control performance during passive hyperthermia.

Objective: To explore clinical features of acute myocardial infarction (AMI) caused by left main (LM) coronary artery lesions and to study the effect of percutaneous coronary intervention (PCI) in relevant patients. Methods: A total of 3514 AMI patients received coronary angiography (CAG) in our hospital from 2000-01 to 2015-12 were studied, those including 36 of infarct-related artery (IRA) as LM. There were 28/36 patients received PCI and 8 received CABG. The clinical features and outcomes in 28 LM disease patients were investigated. Results: The patients included 5 female and 23 male at the mean age of (66.5±8.32) years. There were 16 patients with ST-segment elevation myocardial infarction (STEMI) and 12 with NSTEMI; 21 received primary PCI and 7 had elective PCI; there were 16 patients suffered from cardiac shock at admission. The procedural success rate was 82.1% and the in-hospital mortality was 35.7% (10/28). During (66.1±35.2) months follow-up period, 3 patients had re-NSTEMI and 1 of them received PCI again, 3 patients died. No event survival rate was 66.7%. Conclusion: PCI is feasible for treating AMI patients caused by LM lesions, the in-hospital survival rate was 64.3%; while the MACE occurrence rate during long-term follow-up period has been high.

Objective To explore the feasibility and safety of laparoscopic total colectomy for familial adenomatous polyposis. Methods All 9 patients with familial adenomatous polyposis received laparoscopic total colectomy were analyzed. Results Laparoscopic total colecto-my was successfully performed in all the 9 patients. The operative time,blood loss,flatus and hospitalization were respectively 230~310 min, 80~210 mL,2~3 days and 12~14 days. All patients were followed up for 6~36 months. The postoperative recovery was good. Conclusion Total colectomy can be successfully perfomed with laparoscopic assisted in treating familial adenomatous poliposis. It is a feasible and safe surgical procedure and can significantly improve the quality life.