1.Correlations of Snail and RKIP expressions with Tumor Invasion and Metastasis in Non-Small Cell Lung Cancer
Zhan QI ; Dayun YANG ; Shaowei GAO
Tianjin Medical Journal 2014;(6):585-587
Objective To detect Snail and Raf kinase inhibitor protein (RKIP) expressions in NSCLC (Non-Small Cell Lung Cancer) and their relationship with the pathological characteristics of tumor. Methods Immunohistochemistry was used to detect Snail and RKIP expression in 124 NSCLC samples and 67 paraneoplastic normal lung tissue samples. Snail and RKIP positive expression rates were compared upon clinicopathologic characteristics. Correlation of Snail expres-sion with RKIP expression was also analyzed. Results Positive expression rate of Snail was 79.03%in lung cancer, which is higher than that in normal lung tissue 19.40%(χ2=63.538, P<0.01);positive expression rate of RKIP was 36.29%in lung cancer which is lower than that in normal lung tissue 77.61%(χ2=29.716,P<0.01), Snail and RKIP expressions are corre-lated with tumor differentiation, TNM stage, lymph node metastasis and postoperative survival time ( P<0.05, respectively) . Snail protein expression is negatively correlated with RKIP expression in NSCLC (P<0.05). Conclusion High Snail ex-pression and the low RKIP expression might be important biological markers for invasion and metastasis of NSCLC, which might be used as important prognostic indicators in NSCLC.
2.The basic structure of heavy-ion tumor therapy facility.
Tong WANG ; Ping XIAO ; Shaowei JIA ; Kehong YUAN ; Hongjie YANG
Chinese Journal of Medical Instrumentation 2014;38(6):427-438
Heavy-ions have the similar characteristic of depth-dose distribution with protons, but exhibit enhanced physical and radiobiological benefits. With increasing development in technical and clinical research, more facilities are being installed in the world. At the same time, many critical techniques of heavy-ion therapy facility were optimized and completed. This paper classified and reviewed the basic structure of heavy-ion system equipments, especially the accelerator, gantry, nozzle , TPS.
Cancer Care Facilities
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Heavy Ion Radiotherapy
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instrumentation
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Humans
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Neoplasms
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therapy
3.Location and expression of vitamin D receptor in the duodenum of C57BL/6 mice at different developmental stages
Jia CHENG ; Shaowei LIU ; Wanying CAO ; Yiqi YANG ; Wang YAO
Acta Laboratorium Animalis Scientia Sinica 2017;25(3):320-324
Objective To investigate the relationship of vitamin D with the intestinal development and study the expression of vitamin D receptor (VDR) in the duodenum of C57BL/6 mice at different developmental stages.Methods Quantitative PCR (qPCR),histology using H&E staining and immunofluorescence staining,and Western blotting (WB) were performed to elucidate the expression of VDR in mice intestine at different growth and developmental stages.Results The peak of VDR mRNA expression reached on 21 d.The pathological result showed that VDR mainly distributed in the cytoplasm of epithelial cells and smooth muscle cells in the mouse duodenum.WB result indicated that there was no nuclear translocation of VDR protein in the mouse duodenum.Conclusions This study demonstrates the regularity of expression of VDR in the mouse duodenum during its development,and contributes to understanding the function of VDR in the intestines.
4.Regional distribution and resource allocation of intraocular lens diopter in cataracts on Lifeline Express
Ling WAN ; Fengren DU ; Qing CAO ; Shaowei YANG
Recent Advances in Ophthalmology 2017;37(3):255-258
Objective To investigate the better medical resource allocation scheme by analyzing the overall situation and regional characteristics of intraocular lens diopter in cataract patients on Lifeline Express.Methods The total of 27 663 patients data from seven administrative regions of China on cataract surgery in China Lifeline Express from 2014 to 2016 were collected.All diopter values of IOL were recorded to grasp the overall distribution of IOL diopter in cataract patients,and the distributive difference among administrative regions was analyzed.Results The distribution of IOL diopter in 27 663 cataract patients presented a skewed trend,with peak value in 21-<22 D.The greater frequency of IOL diopter was mainly concentrated in 19-<24 D,accounting for 72.48% of the total.The IOL diopter peak was present in 22-< 23 D in Northeast China,North China,Central China,East China,and the overall waveform offset to a large degree direction.The IOL diopter peak was present in 21-< 22 D in Northwest,Southwest,South China,and the overall waveform showed the trend of small degree direction.The occurrence frequency of IOL with diopter < 10 D was significantly more obvious in Northwest,Northeast China,North China and East China.At the same time,the occurrence frequency of IOL with diopter ≥25 D was significantly more obvious in Central China,Northeast China,East China,North China.Conclusion The IOL diopter values of cataract patients in different administrative regions present a skewed distribution and there are subtle differences between different regions.The results can provide the basis for resource allocation for large scale cataract surgery in each area.
5.Evaluation of the therapeutic efficacy for methamphetamine dependence through visual digital neural molecular imaging techniques
Hongjie YANG ; Shaowei JIA ; Shu HU ; Zhengqin ZHAO ; Zhou GAO
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(4):328-333
Objective To evaluate the therapeutic effects of methamphetamine (MA) dependence and the repairment of DA neuronal function by SPECT corpus striatum DAT visual digital neural molecular imaging techniques.Methods 25 MA dependent patients (BPRS score ≥ 35) were treated by self-designed treatment program for more than 6 months.The clinical therapeutic effects were scored with reducing rate of BPRS.MA dependent patients were examined by SPECT corpus striatum DAT imaging before and after treatment,while healthy volunteers were examined only once.The SPECT corpus striatum DAT images were analyzed visually and quantitatively.Results The reducing rate of BPRS showed that the total effective rate was 80.0%.Visual analysis of SPECT corpus striatum DAT images showed that the distribution of DAT in the corpus striatum was regionally reduced or defected in various degrees before treatment,and was significantly increased after treatment.Quantitative analysis showed that the bilateral striatal V ((19.26 ± 2.85) cm3),m((20.22±2.99) g) and Ra(4.78±0.79) %) of MA dependent patients were significantly lower compared with those of the healthy volunteers(respectively (35.39±4.42) cm3,(37.16±4.64) g and (7.93± 0.86) %) (all P< 0.01) before treatment and were significantly improved (P< 0.01) after treatment (V:(22.80±4.28) cm3,m:(23.93± 4.49) g and Ra:(5.64 ± 0.99) %) with a 76.0% corpus striatum DAT improvement rate.However,the bilateral striatal V,m and Ra of MA dependent patients after treatment were still lower than those of the healthy volunteers (P<0.01).There was no significant difference between the striatal DAT improvement rate and the BPRS reduction rate (P> 0.05).Conclusion SPECT corpus striatum DAT visual digital neural molecular imaging techniques are reliable in the evaluation of the treatment programs for MA dependence and the repair of DA neuronal function.
6.Effects of MAGEA3 inhibition by shRNA on apoptosis in human hepatocellular cancer cells
Wenyu LI ; Ailin GUO ; Suqing YANG ; Shaowei HU ; Jianming WEN
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To investigate the inhibitory effect of vector-based RNA interference(RNAi) on the expression of melanoma associated antigen A3(MAGEA3) protein in hepatocellular carcinoma cells and on apotposis of hepatocellular carcinoma cells.METHODS: A vector for transcribing specific small hairpin RNA(shRNA) targeting MAGEA3 gene was constructed,introduced into hepatocellular carcinoma MEL-ED1 cells by Lipofectamine 2000.The MAGEA3 protein and mRNA expression levels of MEL-ED1 cells were detected by Western blotting and RT-PCR, respectively.The cell apoptosis was studied by DNA fragmentation,electron microscopy,TUNEL assay,and annexin V/PI staining.RESULTS: The vector of RNA interference was successfully constructed and MAGEA3 expression was descreased significantly in MEL-ED1 cells.After the shRNA expression vector was transfected into the MEL-ED1 cells,the expression of MAGEA3 gene was inhibited significantly(by 90%).DNA fragmentation,electron microscopy and TUNEL assay showed classic apoptosis characters in the MEL-ED1 cells transfected with pSilencer-MAGEA3 plasmid with an apoptosis rate of 21.41% ?1.98%,significantly higher than those in the negative control group transfected with pSilencer-neo and in the non-transfected group(both P
7.Study of realgar induced apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs
Jide XU ; Zhixiang HUANG ; Shaowei YANG ; Zhonghui WEN ; Ruifang ZENG
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(09):-
AIM: To observe the realgar induced the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pigs and investigate the mechanism that realgar treated asthma.METHODS: The morphology of apoptosis of eosinophils was observed by Giemsa staining and electron microscope.The rate of apoptosis of eosinophils was assayed by the flow cytometry.RESULTS: The characteristic changes of the apoptosis in both light microscope and electron microscope were shown after 6 hours treatment of realgar.Flow cytometry showed that the rate of apoptosis of the eosinophils was increased with both increasing realgar concentration and prolonging realgar action time to the cells.CONCLUSION: Realgar promotes the apoptosis of eosinophils from bronchoalveolar lavage fluid in asthmatic guinea pig.Realgar induced the apoptosis of eosinophils is one of the causeses for asthmatic treatment.
8.Study on HPLC Fingerprint of Total Flavonoids from Pollen ofBrassica campestrisL.
Chengcheng FENG ; Sude YANG ; Shaowei YUAN ; Zhenzhong WANG ; Yuan BI ; Siyang FAN ; Yifang YANG ; Wei XIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):591-595
In this article, HPLC fingerprint analysis method for total flavonoids from pollen of Brassica campestris L.was established., The HPLC fingerprint was performed on Waters C18 column (250 mm × 4.6 mm, 5 μm), eluted gradiently with the mixture of acetonitrile and 0.4% phosphoric acid aqueous solution at a flow rate of 0.8 mL·min-1. The column temperature was 40℃. The detection wavelength was 320 nm. The HPLC standard fingerprint of total flavonoids from pollen of Brassica campestrisL. was established, and 16 common peaks were calibrated. The method was simple, stable, and reproducible. It could be applied for quality control of total flavonoids from pollen of Brassica campestris L.
9.Application value of SPECT-CT fusion imaging in moyamoya disease revascularization:a clinical study
Hui QI ; Lei ZHAO ; Guangyuan WU ; Siyang ZHENG ; Da HUANG ; Wei YIN ; Hongjie YANG ; Shaowei JIA
Chinese Journal of Cerebrovascular Diseases 2014;(10):516-521
Objective To investigate the application value of identification of the scalp surface locations of cerebral ischemia lesions before direct revascularization for moyamoya disease and to design surgical approaches according to this by using the fusion of single photon emission computed tomography ( SPECT) cerebral perfusion imaging with CT imaging. Methods The clinical data of 13 adult patients with ischemic-type moyamoya disease underwent superficial temporal artery-middle cerebral artery bypass surgery were analyzed retrospectively. SPECT cerebral perfusion imaging was fused with CT imaging of the same machine before procedure. The lesions of ischemia were located on the cortical surface. The surgical approaches were designed at the center of the ischemic lesions. The patients were followed up for 6 to 12 months after procedure. The improvement of clinical symptoms and cerebral perfusion of the patients were observed after operation. Results One patient had perioperative cerebral hyperperfusion syndrome,and the others did not have any perioperative complications. At one-month follow-up, the improvement of symptoms in 4 patients were excellent,in 5 were good,in 4 were fair,and none was poor. At 6 to 12 month follow-up,the improvement of symptoms in 9 patients were excellent,in 4 were good,and none was poor. The postoperative visual SPECT cerebral perfusion imaging analysis suggested that the cerebral perfusion was improved significantly as compared with before procedure in all patients. Quantitative analysis:There was significant difference in target ischemic lesions between preoperative Fb and postoperative Fb ([2. 13±1. 06]% vs. [4. 13±2. 09]%;P<0. 05). There was significant difference between preoperative Fb and Fe ([2. 46±1. 97]% vs. [2. 13±1. 06]%;P<0. 05). The postoperative BFCR was [67. 57±3. 78]%( >50%) , which indicated that the efficacy of the procedure was remarkable. The superficial temporal arteries fed to brain of the patients were observed after procedure by using the head CT angiography. The postoperative head MRI reexamination showed no new infarcts occurred at 6 months. Conclusion Combine SPECT cerebral perfusion imaging with CT imaging to design surgical approach for superficial temporal artery-middle cerebral artery bypass surgery may improve the efficacy and reduce the risks of operation.
10.Influence of different brushing time in oral hygiene in adolescent orthodontic patients and their clinical significances
Te WANG ; Xingfu BAO ; Junxing YANG ; Shaowei LIU ; Hang YU ; Min HU
Journal of Jilin University(Medicine Edition) 2017;43(2):375-380
Objective:To study the changes of oral health indexes in adolescent orthodontic patients under different brushing time, and to provide basis for the orthodontists to give effective dental health promotion in clinic.Methods:A total of 123 adolescent orthodontic patients were included, and they were divided into 1-3min, 3-5 min, 5-7 min and 7-10 min groups based on the brushing time recorded in the period of orthodontic treatment.The relationship between different brushing time and oral health indexes was evaluated 1,3,and 6 months after experiment, such as sulcus bleeding index(SBI),plaque index(PLI), debris index-simplified(DI-S) and enamel demineralization index(EDI).Results:Compared with 1-3 min and 3-5 min groups, the SBI, PLI, and DI-S of the patients in 5-7 min group were significantly reduced(P<0.05).There was no significant differences of SBI, PLI, DI-S , and EDI between 5-7 min group and 7-10 min group (P>0.05).Conclusion:Brushing teeth more than 5 min can improve the oral hygiene significantly and the best time is 5-7 min;the orthodontists should require the patients to brush teeth at least for 5 min.