Objective To realize the expression changes and patterns of lipid metabolism and transport-related genes in rat liver regeneration(LR) at transcriptional level. Methods Genes involved in lipid metabolism and transport were obtained by retrieving the databases data and the related papers.The gene expression changes during LR was checked by Rat Genome 230 2.0 array,and LR-associated genes were identified by comparing gene expression difference between partial hepatectomy(PH) and sham operation(SO) groups. Results One hundred ninety three genes were found to be LR-associated.The number of initially and totally expressed genes occurring in forepart(0.5-4 hours after PH) of LR,prophase(6-12 hours after PH),metaphase(12-66 hours after PH),and anaphase(72-168 hours after PH) was 113,20,66,1 and 250,205,796,293,respectively.The number of their up-regulation and down-regulation was 852 and 630 times,and their expression profiles were classified into 27 types.The subdued transcription levels occur to the bile acid metabolism-involved genes in forepart and prophase of LR;the elevated transcription levels occur to the glucocorticoid catabolism-involved genes in forepart and anaphase;phospholipids synthesis-involved genes were up-regulated in prophase and metaphase,while phospholipids catabolism-involved genes were down-expressed;fatty acid,leukotriene and glycosphingolipid synthesis-involved genes,triglyceride and phosphatidylinositol metabolism-involved genes were up-regulated in metaphase,while glycosphingolipid catabolism-involved genes were down-expressed;the elevated transcription levels occur to the prostaglandin synthesis and fatty acid catabolism-involved genes in metaphase and anaphase;during almost the whole period of LR,genes participating in sex hormone,glucocorticoid and progesterone synthesis,sphingomyelin metabolism and lipid transport were up-regulated,while cholesterol metabolism-involved genes were down-expressed.Conclusion Lipid metabolism and transport during LR were diverse,and they were tightly related with LR.

Objective To explore the effect of nursing intervention on the oral hygiene of vagrant patients with mental problems.Methods Toally 80 vagrant patients with mental problems were saved and treated with oral health care intervention for a period of three months.The pre-intervention effects were prepared with the post-intervention ones in terms of tooth brushing,oral health and periodontal disease related symptoms intervention.Results Before intervention,the rate of tooth brushing was significantly higher and the rate of periodontal disease related symptoms significantly lower than that before intervention (P＜0.05).The scores on physical function limitation,pain and discomfort,psychological discomfort,weakened ability in independent living and the total score after intervention were all significantly lowered as compared to the pre-intervention.Conclusion Oral nursing intervention can improve oral hygiene and oral health of vagrant patients with mental diseases.

Objective Parainfluenza virus is an important pathogen of lower respiratory tract infections in infants and young children.This study was to search for a method for rapid culture and identification of human parainfluenza viruses from nasal swabs. Methods Nasal swab specimens were collected from 0-5 years old children with acute respiratory tract infection.The specimens were inoculated onto 96 plates with prefabricated LLC-MK2 cells and then centrifuged for 1 hour at 3000 r/min and also inoculated using the traditional culture method, followed by addition of virus mainte-nance medium containing 4 μg/mL TPCK trypsin.The cytopathic effect was observed daily, and hemagglutination and blood absorption tests were done at 2, 5, and 8 days after inoculation.In case of posi-tive result of either test, the specimen was subjected to immunofluo-rescence staining. Results Six strains of parainfluenza virus were isolated from the 83 nasal swab specimens, with a positive rate of 7.2%.There was a significant difference in the rate of separation be-tween the rapid and traditional culture methods after 2 days of culturing (7.2%vs 0%, P<0.05).The infected cells produced a cy-topathic effect that characterized by syncytium and crush formation.Hemagglutination and blood adsorption tests were positive at 4℃and negative at the room temperature.Immunofluorescence staining exhibited specific apple green fluorescence. Conclusion The method for rapid culture and identification of human parainfluenza viruses in nasal swab specimens was successfully established, which can be used to obtain and identify parainfluenza viruses with virulence and biological activity in 2 days.

Objective: To investigate the history, risk factors for prognosis of malignant biliary stricture (MBS) patients receiving conservative therapy after endoscopic retrograde cholangiopancreatography(ERCP) and to set up a predictive model for overall survival (OS). Methods: MBS patients who underwent ERCP and conservative therapy in Xijing Hospital and PLA No.451 Hospital from January 2009 to December 2013 were enrolled to the present study. Predictive factors associated with OS were identified in the training cohort by stepwise multivariate Cox regression analysis. A predictive model was then developed and externally validated in the validation cohort. Results: Between January 2009 and December 2013, 152 and 149 patients were eligible to the training and validation cohort respectively. In the training cohort, tumors were mainly originated from bile duct (33.6%), pancreas (23.5%) or ampulla (20.4%). 76.3% (116/152) patients died during the observation period. The median OS for the training population was 5.0 months (3.9-6.2 months). CA19-9≥1 000 U/mL, non-ampulla tumor, metastasis, pre-ERCP total bilirubin≥7 mg/dL and hilar stricture were identified as independent predictive factors of poor OS (all P<0.05). Based on these factors, the COMTH predictive model was developed. The median OS of patients with COMTH>8 in the training and validation cohorts were both 3.0 months, which were significantly shorter than those with COMTH≤8 (10.0 and 6.9 months in the training and validation cohorts respectively, both P<0.001). Conclusion The prognosis of MBS patients undergoing ERCP is poor. The survival chance of patients with COMTH>8 is even more dismal.