Objective To set up a new method for fast determining and identifying Xintong Oral Liquid by acousto-optic tunable filter-near infrared(AOTF-NIR)spectroscopy.Methods Identifying method by NIR spectroscopy combined with principal component analysis(PCA),and determining model of qualitative and quantitative analyses based on PLS1 algorithm.Results The XT-C identifying model could be used to identify Xintong Oral Liquid,the determining model of qualitative and quantitative analyses with better accuracy,RMSEP of the models for puerarin was 0.137 1,the determination coefficients was R2=0.984 5.The correlation coefficient of the true value and predication value from validation was r2=0.996 4.The average recovery of the predication set was 101.9%.RSD for precision was 1.98%,RSD for stability was 1.57%.Conclusion The method is a quick and simple assay technique with low cost,which is able to be used in the qualitative and quantitive analyses on Xintong Oral Liquid.

AIM:To establish a new method of rapid analysis of puerarin in Xintong Oral Liquid by acousto-optic tunable filter-nearinfrared spectroscopy. METHODS: HPLC was used as a reference method to determine puerarin content in Xintong Oral Liquid. Calibration model based on partial least squares(PLS1) algorithm was developed to correlate the spectra and the corresponding values determined by the reference methods. RESULTS: The root mean square error of prediction(RMSEP) of the model for puerarin was 0.137 1. The determination coefficient was R～2=0.984 5. The correlation coefficient of the true value and predication value from validation was r～2=0.996 4. CONCLUSION: The method is a quick, simple and low cost assay and able to be used in quantitative analysis of Xintong Oral Liquid.