ObjectiveTo cultivate high-qualified talents with innovation and comprehensive clinical skills under the guidance of Chinese standard of undergraduate medical education.Methods Courses of clinical thinking and misdiagnosis,medical record writing,communication between patients and doctors were provided.Objective structured clinical examination (OSCE) with standard patients (SP) was employed.Results Satisfactory scores were obtained among relevant tests,the passing rates of specialist qualification examination and practicing doctors clinical skills test were increased from 44.61％ and 59.18％ to 66.66％ and 95.45％.ConclusionThe implementation of correlative courses and OSCE can comprehensively promote the medical students' clinical competency and pave the way for their future work and practicing doctors' qualification exam.

Objective To investigate the proliferation and invasion of human pancreatic cancer cells after Stat1 gene silenced or enhanced and to explore the possible mechanisms.Methods BxPC-3 cells were divided to three groups: Stat1 group transfected with specific Stat1 plasmid, Si Stat1 group transfected with specific Stat1 small interfering RNA (siRNA), and control group.Western blot was used to detect the expression of Stat1, VEGF, MMP-2 and MMP-9 in BxPC-3 cells.MTT method was used to detect the proliferation of BxPC-3 cells.Transwell was used to detect the invasion of BxPC-3 cells.Results The proliferation and invasion were significantly enhanced in pancreatic cancer cells after Stat1 gene silenced, and the expression of VEGF, MMP-2 and MMP-9 was increased.The proliferation and invasion were significantly decreased in pancreatic cancer cells after Stat1 gene enhanced.Conclusions Stat1 inhibits the proliferation and invasion of pancreatic cancer cells.VEGF,MMP-2 and MMP-9 possibly play a role in this process.

Objective To investigate the expression and clinical significance of transcriptional activator (STAT) 1 and matrix metalloproteinases (MMP)-2 in pancreatic cancer (PC). Methods The expressions of STAT1 and MMP-2 were
detected by S-P immunohistochemical methods in 115 PC patients and whose age, tumor size, lymph node metastasis, TNM stage, differentiation and survival time were also collected. Results STAT1 protein was high expressed in 52 cases and MMP-2 was high expressed in 56 cases, which were significantly related with different clinical stages, tumor differentiation and lymph node metastasis in patients with PC (P<0.05). STAT1 expression was negatively correlated with MMP-2 expression (rs =-0.50, P<0.01). Lymph node metastasis, high level of clinical stage, low expression of STAT1 showed reduced postoperative survival time in patients with PC. Conclusion The lower expression of STAT1 and higher expression of MMP-2 play an important role in the progression of PC. The expression of STAT1 has practical value in the evaluation of prognosis in patients with PC .

Objective To evaluate the relationship between thyroid nodules combined with Hashimoto's thyroiditis(HT) and the risk of thyroid cancer.Methods This retrospective study included 1212 patients undergoing thyroidectomy in the Second Hospital of Tianjin Medical University from Jan.2010 to Jul.2017.The incidence of thyroid cancer in those thyroid nodules with and without HT based on the postoperative pathological results was compared.Results Among 843 patients of thyroid benign nodules,15.3％ cases were complicated with HT.Of all the patients with malignant tumors,365 cases were PTC,accounting for 96.5％ of all malignant tumors,and among whom,10.8％ were complicated with HT.Of the 169 cases of thyroid nodules combined with HT,23.7％ patients had PTC.For patients with thyroid nodules without HT,30.3％ had PTC.The difference had no statistical significance (x2=3.08,P＞0.05).Conclusion Thyroid nodules complicated with HT doesn't increase the incidence of thyroid cancer.