Objective To synthesize a new-type thermosensitive liquid embolic material and to investigate the feasibility of using this material to occlude cerebral arteriovenous malformations (AVMs).Methods The copolymer was synthesized with N-isopropylacrylamide (NIPAM) and N-n-propylacrylamide (NNPAM),and it's physical and biological properties were estimated.The embolization of AVMs model in vitro by using this copolymer was conducted and the results were analyzed.Results The new-type copolymer possessed unique thermal behavior of lower critical soluble temperature (LCST),and it was water-soluble and non-adhesive with better biocompatibility.The successful embolization of AVMs model could be reliably obtained.Conclusion The copolymer synthesized by the authors is a new-type liquid embolic agent suitable for endovascular embolization of cerebral AVMs in vitro.Based on the results in experiment animals having been reported in medical literature,this copolymer can be further used in clinical research.

Objective To investigate the prevalence of trematode Aspidogastrea in freshwater mussels in Anhui Province, China. Methods The freshwater mussels living in different water areas within Anhui territory were harvested and dissected from April to May,2015. Then the flukes were collected from the pericardial cavities of the clams into a petri dish containing small amount of saline. After rinsing,the flukes were transferred into a bottle containing 70%ethanol for following identification by staining of the specimens prepared as previous protocol. Results A total of 3 007 calms in 12 species,including Unio doug?lasiae(603),Acuticosta chinensis(90),Lamprotula caveat(150),Lamprotula leai(250),Lanceolaria grayana(47),An?odonta pacifica(60),Anodonta woodiana(350),Cuneopsis heudei(100),Solenaia oleivora(150),Cristaria plicata (567),Hyriopsis cumingii(550)and Arconaia lanceolata(90)were dissected,in which 1 467 mussels were infected with the trematode Aspidogastrea,with an infection rate of 48.79%(1 467/3 007). Totally,7 306 flukes belonging to 3 genera under the subclass of Aspidogastrea were isolated. These trematodes were genera of Aspidogaster,Lophotaspis and Cotylaspis. The in?fection density was from 1 to 128 capita for individual positive clam,with a mean infectiosity of 4.98(7306/1467)for each mus?sel. Conclusion The freshwater bivalves living in different water areas in Anhui Province are infected with the trematode Aspi?dogastrea. These flukes were identified as Aspidogaster sp. Lophotaspis sp. and Cotylaspis sp. belonging to subclass Aspidogas?tridae of family Aspidogastrea.

We reported a case of adult Drosophila melanogaster parasitized in nasal cavity of a 81-year-old woman who was living in Xuancheng City, Anhui Province now. She was admitted for treatment of cerebral infarction and water accumulation in the lungs in 2014 June. The patient was also suffering from secretory otitis media, a history of hypertension and heart stents were placed in 2007. A foreign body was found in the left nasal cavity during the preoperative examination process, and then the part of the inflammatory tissue was removed through the nasal endoscopy, and sent to our department for identification. There are three adults of Drosophila in paraffin-embedded biopsy specimens. The parasites length is approximately 3mm, with huge red compound eyes. The end of the body is tip, with 5 ring lines in back, has no dark spots. The abdomen of the parasites have seven sections. Tarsus of foot I have no sex comb on base, and they are male adult of Drosophila melanogaster after identification. After a thorough reviewing of medical history, we knew the patient began to sneeze violently and frequently six years ago. But there was no clear or purulent nasal discharge flowing, therefore did not attract attention. After removing the parasites the sneezing symptoms were relieved, and had no abnormal symptoms in the follow-up 6 months.
Aged, 80 and over ; Animals ; Drosophila melanogaster ; growth & development ; Endoscopy ; Female ; Foreign Bodies ; diagnosis ; parasitology ; Humans ; Male ; Nasal Cavity ; parasitology

Objective To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City so as to provide the evidence for its prevention and control. Methods From March to June and September to De?cember in 2014 dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated identified and counted. Results Totally 360 samples were selected and 169 samples 46.94% had mite infestation. Pyroglyphidae accounted for the most in the population with the breeding rate of 45.00%. Totally 18 504 mites were found and Dermatophagoides farina and Dermatophagoides pteronyssinus were the dominant species with the composition ratios of 30.21%5 590 mites and 17.83% 3 300 mites respectively. The third?ranked kindergartens were in the most serious situation con?cerning with 81.67% 98/120 of the breeding rate of Acaroid mites. The differences were statistically significant among the kin?dergartens with different ranks F=6.048 χ2=73.523 both P<0.05 . There were no significant differences about the Aca?roid mite population among different grades in kindergartens F=0.132 χ2 =2.377 both P>0.05 . Conclusion Acaroid mites in the kindergartens in Wuhu City were in serious condition and the population and species composition of Acaroid mite community is influenced by the human behavior and indoors environments.

Objective To investigate the epidemic situation of Metorchis orientalis in Wuhu City Anhui Province. Meth?ods Three lakes Fengming Longwo and Kui lakes were selected in Wuhu City and the poultries around the lakes and fresh?water fishes in the lakes were captured to test the infection of M. orientalis and the infection rates were calculated. Results The adult worms of M. orientalis were found in gall bladder and hepatic duct of domestic ducks with infection rate of 16.7%. The metacercariae of M. orientalis were discovered in the muscle of Pseudorasbora parva and Pseudogobio rivularis with infection rates of 7.13%and 3.38%respectively. Conclusion M. orientalis is endemic in Wuhu City which should be paid enough at?tention to the animal husbandry aquaculture and medicine.

Objective To investigate whether there is Blomia tropicalis breeding in the flour warehouse of food factory in a civil aviation airport,and,if there is,to observe the morphological structure of the mite. Methods The flour samples were col-lected from the flour warehouse of food factory in a civil aviation airport. The breeding mites were isolated from the samples by di-rect microscopy and made into specimens to observe with a light microscope. Results Female Blomia tropicalis was found in the collected flour samples. Under the microscope,the mite was nearly spherical,and it had no tergum and claw. Its apodemeⅠwas developed and connected in the midline. The reproductive hole was located between the foot Ⅲand IV level and was cov-ered with a pair of oblique genital folds. The footⅢandⅣhad no solenoids. The copulatory pouch was a long,slightly curved tube,extending from the end of the mite. Conclusions Blomia tropicalis has been found in the flour warehouse of a food factory in the civil aviation airport. The main distinguishing feature of this mite is that it has no tergum,its feet have no pectinate inferi-or tarsus scales or claw,the feetⅠgenu has only one solenidia,and feetⅢandⅣhave no solenidias.

Objective To investigate the breeding quantity and average breeding density of Gohieria fuscus in the grand dust flour of a flourmill and explore the prevention and control measures. Methods A certain amount of grand dust flour was collect-ed from a flourmill,and it was sieved. The powder was placed into the glass pan,and the mites were sought out under an optical microscope and made of specimens. The mites were identified on the basis of literature. Results In this survey,400 g samples were collected from 4 habitats. The average breeding density of mites was 3516/g. The mite was identified as Gohieria fuscus. Conclusions Gohieria fuscus is one of the widely distributed stored mites. It impacts the stored food and reduces the quality of food. In addition,the mite affects human health. Therefore,the preventive measures should be taken.

AIM: To investigate the changes on immunological function in antigen-sensitized mice injected by dermatophagoides farinaeliposomes (DF) subcutaneously. METHODS: BALB/c mice were sensitized with DF antigen injected subcutaneously. After injected by DF liposomes with 1, 3, and 9 ?g?g -1 dosage respectively, the total IgE, total IgG, DF specific IgE(sIgE), DF specific IgG (sIgG), IL-4, and IFN-? were detected by ELISA. RESULTS: The total IgE, sIgE, total IgG, and sIgG in the serum of sensitized mice increased, the level of IFN-? reduced and IL-4 increased. After injected subcutaneously by DF liposomes, the total IgE, sIgE and IL-4 in the serum of mice of all the treatment groups reduced, and IFN-? raised, and the total IgG, sIgG raised in the serum of mice administrated with small and medium dosage, but the low dosage was more obvious. CONCLUSION: DF liposomes have some effects on the immunological function in mite-sensitized mice in the low dosage.

Objective To prepare biodegradable microspheres of Schistosoma japonicum recombinant glutathione-S-transferase. Methods By using the constructed expression plasmid pET28a(+)-SjGST, fusion protein of Schistosoma japonicum recombinant glutathione-S-transferases were expressed in LB by E.coli BL21,rSjGST fusion protein was purified through column of resin,and then the fusion protein concentration was detected; by using an oil-in-oil emulsion-solvent extraction method, the rSjGST biodegradable microspheres were prepared in PLGA (75/25).The properties of microspheres including shape、diameter、diameter’s distribution and span were assayed under microscope. Results The concentration of protein after purification was 8.323 mg/ml, and the microspheres were spherical and showed normal distribution.The diameter was (7.3?1.2) ?m,the span was 0.52,the load of the rSjGST was 7.62%. Conclusion Biodegradable microspheres of Schistosoma japonicum recombinant glutathione-S-transferase are successfully prepared, which provides the basis for further studies on protective immunity of mice.

Objective To study the suitable dose of intracoronary adenosine (AD) on fractional flow reserve (FFR) measurement in Chinese patients with angiographic coronary artery disease. Methods FFR was measured in 32 patients with 40 moderate coronary stenosis. Boluses of intracoronary AD at increasing doses of 60μg (A1), 80μg (A2), 100μg (A3) and 120μg (A4) were randomly administered. FFR values, symptoms, systemic effects and development of atrioventricular block were recorded. Results FFR value decreased significantly by 8.99%(A1), 11.24%(A2), 13.48%(A3) and 13.48%(A4) compared with the baseline distal coronary pressure/aortic pressure (0.891±0.044, all P<0.001). A3 and A4 showed significantly lower FFR values than A1 (t=6.331、6.343, all P < 0.001),A2 (t=2.974、3.058, P=0.005、0.004). Positive rates of an FFR of<0.75 were 30.0%(n=12), 32.5%(n=13), 35.0%(n=14) and 35.0%(n=14) in A1, A2, A3 and A4. A total of 13 patients(40.6%) reported at least one side effects. Conclusions This study suggests a dose-response relationship for intracoronary AD on the measurement of FFR in Chinese patients with angiographic coronary artery disease. The suitable dose of bolus of intracoronary AD is 100μg.