Objective To assess the prevalence and presentation of low urinary tract symptoms (LUTS) in Chinese men. Methods A random sample was obtained from men dwelling at communities,factories,institutions and colleges in a district in Wuhan City.By completion of the questionnaire and exclusion of individuals with operation and trauma history,2811 men (age range,20～82 years) were assigned into 6 groups according to 10-year age stratum.Frequency of symptoms and severity of discomfort were assessed using the International Prostate Symptom Score (IPSS). Results The 2811 men accounted for 71.7% of the surveyed population. The occurrence of LUTS was as follows.In 20～29 year group,only 5 (0.6%) of 798 men had mild LUTS (IPSS,1～7).In 30～39 year group,55 (12.1%) of 453 men had mild LUTS.In 40～49 year group,143 (26.6%) of 537 men had LUTS;of them 118 were of mild LUTS,20 of moderate (IPSS,8～19),5 of severe (IPSS,20～35).In 50～59 year group,244 (52.5%) of 465 men had LUTS;of them 109 were of mild,117 of moderate,18 of severe.In 60～69 year group,240 (65.6%) of 366 men had LUTS;of them 78 were of mild,138 of moderate,24 of severe.In≥70 year group,162 (84.4%) of 192 men had LUTS;of them 47 were of mild,68 of moderate,47 of severe.Differences between age groups in incidence rates of LUTS were significant (?2=998.385,P

Objective To investigate the position and distribution of the neurovascular bundle at the lateral border of the prostate in adult male. Methods The neurovascular bundle at the lateral border of the prostate was observed on the remaining 25 sides of the medially dissected 18 male formalin-preserved cadavers with an operative microscope.The neurovascular bundle at the lateral border of the prostate of an adult male cadaver was explored with hematoxylin and eosin staining and nitric oxide synthase (nNOS) immunohistochemistry staining. Results The neurovascular bundle,which stemed from the pelvic plexus,together with the corresponding vessels were formed and divided into 2 branches to pass the lateral border of the prostate.One of them traveled between the posterolateral prostate and anterolateral rectum.The other traveled between the bladder neck and the prostate to reach the lateral border of the puboprostatic ligament.After 2 nerve branches of the neurovascular bundles pierced the urogenital diaphragm, they met together to form a nerve bundle again.Two bundles and the urogenital diaphragm formed a triangle area at the lateral border of the prostate.There was no nerve and vessel in this triangle area.The nerve branches at the lateral border of the prostate contained nerve cell bodies and fibers that stained positive for nitric oxide synthase. Conclusions There are 2 neurovascular bundles at the lateral border of the prostate.One of them travels between the posterolateral prostate and anterolateral rectum.The other travels between the bladder neck and the prostate to reach the lateral border of the puboprostatic ligament.They contain nerve ganglia that stain positive for nitric oxide synthase.

OBJECTIVETo elucidate the immunomodulatory mechanism of phenylethanoid glycosides from the seeds of Plantago asiatica by testing its effects on the maturing of murine bone marrow derived dendritic cells (DCs).

METHODMonocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10% FBS, rmGM-CSF and rmIL-4.50 mg x L(-1) acteoside or isoacteoside was added to cells on day 6 of culture for 24 h. The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

RESULTBoth acteoside and isoacteoside could increase the expression of CD11c, CD86, MHC II and CD80 on DCs surface. The ability of unstimulated DCs to uptake FITC-dextran was higher than that of phenylethanoid glycosides or LPS treated DCs.

CONCLUSIONBoth acteoside and isoacteoside could induce maturation of murine dendritic cells.

Animals ; B7-1 Antigen ; immunology ; metabolism ; B7-2 Antigen ; genetics ; immunology ; Cells, Cultured ; Dendritic Cells ; drug effects ; immunology ; Gene Expression ; drug effects ; Glycosides ; pharmacology ; Male ; Mice ; Mice, Inbred BALB C ; Phagocytosis ; drug effects ; Plant Extracts ; pharmacology ; Plantago ; chemistry ; Seeds ; chemistry

Objective:to understand the current situation of academic research misconduct by analyzing the investigation results of a number of medical scientific research integrity cases, to strengthen the construction of scientific research integrity in medical institutions.Methods:Select the investigation and handling results of 310 medical scientific research integrity cases disclosed by the National Health Commission in 2021, and analyze the basic information of the case papers, the types and characteristics of misconduct behaviors involved, and the handling results of each institution.Results:This batch of problematic papers was widely distributed, including particular hospitals from a certain region or city level hospitals. Identified misconduct included fabrication of the research process, falsification of research data, purchasing, ghost-writing, third-party submission, and inappropriate authorship. Responsible persons were severely punished.Conclusions:Long-term mechanism for the prevention of academic misconduct should be established with collaboration among administrative department, medical institutions and research personnel, to deal with academic misconduct more systematically.

The number of investigator initiated research (IIR) is increasing. But the recognition and management of IIR in China is still in its infancy, and there is a lack of specific and operable guidance for the implementation process. Based on our practical experiences, previous literature reports, and current policy regulations, the authors took prospective IIR as an example to summarize the implementation process of IIR into 14 steps, which are as the following: study initiation, ethical review, study registration, study filing, case report form design, database establishment, standard operating procedure making, investigator training, informed consent, data collection, data entry, data verification, data locking and data archiving.