Objective To observe the effect of Weiyanyin on Substance P(SP) in gastric mucosa of experiment bile reflux gastritis rats. Methods The model of experiment bile reflux gastritis was established by gavage of reflux liquid. Compared with Domperidone, the effect of Weiyanyin on pathological histological features and the mucosal SP content of bile reflux gastritis rats was observed. Results The gastric mucosa of rats in model group was damaged, and alopecia of gastric mucosa, and infiltration of a large number of inflammatory cells could be seen.In most of the rats in the model group, atypical hyperplasia and intestinal metaplasia were present. Meanwhile, the content of SP was decreased( P

The duck circovirus (DuCV) infection in sick ducks from Fujian Province was investigated. The liver samples of 43 sick Muscovy ducks with infectious serositis were collected from 12 duck farms in Fujian Province.Based on the published sequences of DuCV, two primers were designed for the detection of DuCV and four pairs of primers were designed to amplify four overlapping fragments that cover the complete genome of DuCV. The specific PCR products were amplified from positive samples. The fragments were then cloned into pMD18-T vector and sequenced, and the full length genomic sequence of the FJ0601 isolate of DuCV was obtained. PCR analysis showed that the proportion of ducks which were positive for circovirus was 79% and 10 out of the 12 farms were positive. Sequence analysis showed that the complete genome of DuCV-FJ0601 was 1988 bp and possessed features common to the family Circoviridae which included a stem-loop structure and the Rep protein motifs. Homology analysis showed that FJ0601 isolate of DuCV had 97.3%～97.5% nucleotide sequence identity to all the four Taiwan isolates (TC1/2002, TC2/2002, TC3/2002, TC4/2002), 82.9% identity to the America (33753-52) isolate and 82.3% identity to the Germany isolate. Phylogenetic analysis with Clustal W, however,showed that FJ0601 isolate of DuCV was on a common branch with Taiwan isolates, and Germany and America isolates belonged to the other branch.

Objective To investigate the application value of general anesthesia combined with epidural anesthesia in patients with cervical cancer after laparoscopic hysterectomy.Methods From January 2014 to November 2016,64 patients with cervical cancer in Beijing Ditan Hospital were selected and randomly divided into control group and study group,with 32 cases in each group.Both two groups were treated with laparoscopic hysterectomy,and the control group was treated with general anesthesia,the study group was treated with general anesthesia combined with epidural anesthesia.The amount of general anesthesia,the time of surgery and postoperative recovery time,the incidence of adverse reactions,preoperative and postoperative oxidative stress－related indicators[metabolic enzyme monoammine oxidase A (MAOA),enzyme tyrosine hydroxylase (TH),glutathione peroxidase (GSH－Px),5 －hydroxytryptamine (5 －HT)]were statistically compared between the two groups.Results The operative time,postoperative recovery time and doage of atracurium,remifentanil,propofol in the study group were less than those in the control group (t＝6.928,11.205,16.966,7.798,16.839,all P＜0.01).There were no statistically significant differences in the MAOA,TH,GSH－Px and 5 －HT levels between the two groups before treatment (all P＞0.05),but after operation, the MAOA,TH and 5 －HT levels in the study group were significantly lower than those in the control group,the GSH－Px level was significantly higher than that of the control group,the differences were statistically significant (t＝28.585,26.346,10.850,21.827,all P＜0.01).The incidence rates of cardiac tachycardia[18.75%(6/32)],vomiting and nausea[15.63%(5/32)],and restless[9.38%(3/32)]in the study group were lower than those in the control group[43.75%(14/32),37.50%(12/32),40.63%(13/32)],the differences were statistically significant (χ2＝4.655,3.925,8.333,all P＜0.05).Conclusion General hysterectomy combined with epidural anesthesia in patients with cervical cancer after laparoscopic hysterectomy can effectively reduce the postoperative oxidative stress,reduce the dosage of narcotic drugs and the time of operation and wake up,and the incidence rate of adverse reactions is low.

OBJECTIVE: To assess the value of non-invasive prenatal testing (NIPT) for the detection of fetal chromosomal aneuploidies in women with twin pregnancy. METHODS: A total of 2473 women with twin pregnancy underwent the NIPT test to assess the risk for fetal chromosomal aneuploidies from January 2016 to September 2019. Those with a high risk by NIPT were confirmed by amniocentesis or chorionic villus sampling. All cases were followed up to evaluate the positive prediction value of NIPT for twin pregnancies. RESULTS: Among the 2473 women, the NIPT test has identified 31 cases (1.25%) with a high risk for fetal chromosomal aneuploidies, which included 5 cases of trisomy 21, 1 case of chromosome 21 deletion, 4 cases of trisomy 18, 7 cases of sex chromosome abnormality and 14 cases of microdeletion and microduplication. By invasive prenatal diagnosis or chromosomal karyotyping analysis of neonates, 5 cases of trisomy 21, 3 cases of trisomy 18, 1 case of sex chromosome abnormality, and 2 cases of microdeletion and microduplication were confirmed, which yielded a positive predictive value of 100%, 75%, 25% and 25%, respectively. CONCLUSION NIPT can be used for the screening of fetal chromosomal aneuploidies in women with twin pregnancy with high accuracy. The method is non-invasive, safe and effective for the screening of fetal chromosomal aneuploidies, in particular trisomy 21.
Aneuploidy ; Chromosome Disorders ; Female ; Humans ; Infant, Newborn ; Pregnancy ; Pregnancy, Twin ; Prenatal Diagnosis ; Trisomy ; Trisomy 13 Syndrome ; Trisomy 18 Syndrome

OBJECTIVE: To explore whether it is necessary to choose NIPT-plus for the prenatal screening of pregnant women. METHODS: The results of NIPT and NIPT-plus sequencing data, fetal DNA concentration, prenatal diagnosis and pregnancy outcome of 50 pregnant women were compared. RESULTS: Compared with NIPT, NIPT-plus attained similar fetal DNA concentration and a 4.4-fold increase in sequencing data. NIPT was able to detect 4 cases of 21-trisomy, 2 cases of 18-trisomy, and 9 cases of sex chromosome aneuploidies (SCAs) signaled by NIPT-plus, but missed one 18-trisomy, and failed to detect rare chromosome aneuploidies (RCAs) and microdeletion/microduplication syndromes (MMS). The PPVs of NIPT-plus for 21-trisomy, 18-trisomy, SCAs, MMS and RCAs were 100%, 100%, 44.4%, 30.4% and 0%, respectively. And those of NIPT for 21-trisomy, 18-trisomy, and SCAs were 100%, 100%, and 44.4%, respectively. CONCLUSION It is necessary for pregnant women to select NIPT-plus to improve the detection rate of common trisomies, SCAs and disease-specific MMS, therefore reduce the occurrene of birth defect.
Aneuploidy ; Female ; Humans ; Pregnancy ; Pregnant Women ; Prenatal Diagnosis ; Trisomy ; Trisomy 13 Syndrome ; Trisomy 18 Syndrome