Objective The a seek in ideal of iatrotechnique is treatment of Traumatic Cerebral Tinnitus(TCT).Methods Use the pioneering of Laser Meridian Points Therapy(LMPT).The domestic He-Ne laser therapeutic instrument was used with optical fiber wave length 632.8nm,output power 2~30mW,therapeutic power quartz optical fiber at the end of fiber power 1~10 mW,diameter of optical patch at the end of the optical fiber 0.2cm,optical patch area 0.0314 cm2,power density 31.85~318.47mW/cm2.Use the optical fiber end of the instrument to contact and irradiated the following points:Yifeng(SJ 17),Yiming(Extra),Fengch'ih(GB 20),Yingyou,T'ingkung(SI 19),T'inghui(GB 2),Erhmen(SJ 21),Paihui(Du 20),et al.Each point each time was irradiated for 5~15 minutes of laser dosage(energy density)9.555~286.623J/cm2,once a day,7~14 days a course of treatment.Results 262 cases(503 ears)TCT(patients)of 215 cases(82.06%)414 ears(82.31%)showed tinnitus disapperance,45 case(17.18%)86 ears(17.10%)showed marked or some therapeutic effectiveness,2 case(0.76%)3 ears(0.59%)was ineffective.Follow-up survey 1~9 years of 157 cases 284 ears,found 141 case(89.81%)255 ears(89.78%)had their tinnitus disappeared,15 case(9.55%)27 ears(9.51%)showed marked effectiveness or some effectiveness,1 cases(0.64%)2 ears(0.70%)was ineffective.Conclusions LMPT for the treatment of TCT is the pioneering technique we have developed.Better than any other therapies at present and having good short-term effectiveness and stable and ideal long-term effectiveness.LMPT is worthwhile for further study and popularized clinical application.

Objective To seek an ideal treatment for tinnitus.Methods Use the pioneering of Laser Meridian Points Therapy(LMPT).The domestic He-Ne laser therapeutic instrument was used with optical fiber wave length 632.8nm,output power 2～30mW,therapeutic power quartz optical fiber at the end of fiber power 1～10mW,diameter of optical patch at the end of the optical fiber 0.2cm,optical patch area 0.0314cm～2,power density 31.85～318.47mW/cm～2.Use the optical fiber end of the instrument to contact and irradiate the following points:Yifeng(SJ 17),Yiming(Extra),Fengch'ih(GB20),Yingyou,T'ingKung(SI 19),T'inghui(GB 2),Erhmen(SJ 21),Paihui(Du 20),et al.Each point each time was irradiated for 5～15 minutes of laser dosage(energy density)9.555～286.623J/cm～2,once a day,7～14 days a course of treatment.Results 1808 cases(3037 ears)tinnitus(patients)of 1469(81.25%)cases 2686(81.22%)ears showed disapperance,313(17.31%)cases 577(17.45%)ears showed marked or some therapeutic effectiveness,26(1.44%)cases 44(1.33%)ears was ineffective.Follow-up survey 1～9 years of 1139 case 2043 ears found 936(82.18%)cases 1678(82.14%)ears had their tinnitus disappeared,188(16.51%)cases 340(16.64%)ears showed marked effectiveness or some effectiveness,15(1.32%)cases 25(1.22%)ears was ineffective.Conclusions LMPT for the treatment of tinnitus is the pioneering technique we have developed.Better than any other therapies at present and having good short-term effectiveness and stable and ideal long-term effectiveness,LMPT is worthwhile for further study and popularized clinical application.

Objective To investigate the feasibility of labeling iris pigment epithelial(IPE)cells of rabbits with 5-(and 6-)carboxyfluorescein diacetate succinimidyl ester(CFSE). Methods Enzyme-assisted microdissection was used to isolate the cultured rabbit′s IPE cells.The third or forth subcultured IPE cells were incubated with 2.5,5,10,20,and 40 ?mol/L of CFSE for 1,5,and10min respectively.The fluorescence intensity was detected by flow cytometry,and the leakage of CFSE and its dyeing were observed by fluorescence antibody labeling. Results Incubation with 20 ?mol/L CFSE under 37℃for1minute was the most optimal condition for IPE cells labeling.The coloration of IPE cells stained by CFSE lasted 4 weeks.There was no leakage of dye from labeled rabbit IPE cells to non-labeled human IPE cells in mixed culture process. Conclusion With the advantages of high rate of dyeing,long time of tracing,safety and convenience,CFSE can be used as a new method to label the rabbit′s IPE cells.

Objective To explore the association between the concentration of the high-sensitivity C-reac-tive protein(hs-CRP) and acute myocardial infarction(AMI).Methods The concentration of hs-CRP and serum cholesterol were determined by latex-enhanced immunoturbidimetry in 356 patients with AMI and 356 healthy con-trols.Results The concentration hs-CRP was significantly higher in patients with AMI[(18.6±6.9)mg/L ]than in healthy controls[(2.5±1.7)mg/L,P<0.01].and so the concentration of triglyceride(TG)and low-density lip-oprotein(LDL-C)(P<0.05).Conclusion The concentration of serum hs-CRP can predict the risk of AMl with higher prediction value than TG and LDL-C.

or ≤50 in each patient. 5-year survival curves were estimated with the Kaplan-Meier method and compared by the log-rank test. ResultsWT5”BZ Analysis revealed that among node-negative patients, the 5-yerar's survival rate was 23% higher in patients with more than 50 nodes removed than those with less than 50. For node-positive patients, the difference of 5-year′s survival rate between the related two subgroups reached 36%.WT5”HZConclusion The number of lymph node resected has great impact on the long term survival of patients with colorectal cancer.

Objective To explore the causes and the consequences of iatrogenic bile duct injury as well as experiences in its diagnosis and treatment. Method The clinical data of 28 in-patients who suffered from bile duct injury followed by cholecystectomy during January 1995 to September 2011 were collected and retrospectively analyzed. Result Bile duct injuries were found and diagnosed in al 28 in-patients during the processes of reoperation. Different treatments were carried out. 8 cases with mild injury were treated by placing the abdominal cavity drainage. 6 cases with bile duct split were repaired and set up with T tube drainage. 4 cases were initially treated by bile-intestinal Roux-y anastomosis. 1 case received repair operation of bile-intestinal Roux-y anastomosis after the external drainage of bile. 9 cases were initially treated with end-end bile duct anastomosis and 7 of 9 cases with concurrent anastomotic stenosis were performed repairing re-operations twice or more than twice. Among 7 cases, 1 case was performed with the operation of umbilical vein flap repair while 6 cases were treated with bile-intestinal Roux-y anastomosis repair. All cases in this observation were followed up to two years after repairing surgery:2 patients died and the rest 26 patients recovered after treatment. Conclusions The initial repairing operation is critical for iatrogenic bile duct injury and bile-intestinal Roux-y anastomosis should be the first choice of therapy,and it should be operated by experienced surgeons.

Objective To investigate the impact of planning parameter settings on plan quality and delivery efficiency of VMAT for nasopharyngeal carcinoma with two treatment planning systems (TPS),as references for clinic plan optimization. Methods 25 patients with nasopharyngeal carcinoma were selected and planned for SIB?VMAT treatment. The same planning aims were used in the two kinds of TPS ( TPS?1 and TPS?2). Multiple planning parameters were set for plan optimization. Dose distribution to the target volumes and organs at risk,monitor unit ( MU) and delivery time were compared. Paired t?test or one?way ANOVA was used for the data which was in accordance to normal distribution;otherwise, nonparametric Wilcoxon signed rank test or nonparametric Friedman test was used. Results More segments lead to better plan quality and less MU but longer delivery time ( Minor impact was observed when segment number was larger than 120) in TPS?1,while it had little impact on both plan quality and delivery efficiency in TPS?2. Comparing to single?arc plans,dual?arc VMAT achieved no significant benefit in plan quality but had more MU and longer delivery time in TPS?1 ( P= 0?000 ) . However, dual?arc VMAT plans had better dose distribution in TPS?2, decreased the maximum and mean dose for spinal cord in 3?9% and 13?7%respectively (P=0?000,0?000).Changing the settings of maximum or minimum dose rate did not affect the plan quality in both of the tested TPSs. Increasing the maximum or minimum dose rate reduced the delivery time but the latter increased the number of MU ( P=0?000,0?000) . Conclusions VMAT plan quality and delivery efficiency is affected significantly and differently by planning parameter settings for two TPSs. Trial test should be conducted for different TPS to determine the optimal parameter settings.

Objective To observe the effects of Taohechengqitang combined with danazol on CA125 and gonadal hormone in the treatment of patients with endometriosis.Methods 77 cases from July 2014 to January 2015 in department of obstetrics and gynecology of Daqing Longnan hospital were selected and divided into observation group of 40 cases and control group of 37 cases according to double-blind randomized.The observation group were treated with Taohechengqitang joint danazol treatment, and the control group were treated with danazol alone.The estradiol ( E2 ) , progestogen (P), cancer antigen 125 (CA125), thromboxane B2 (TXB2), endometrial cysts size and dysmenorrheal score pre-and post-treatment between two groups were compared.Results The contents of E2 and P post-treatment in observation group were lower than those in control group (P<0.05).The serum TXB2 and CA125 levels post-treatment in observation group were lower than those in control group (P<0.05).The endometrial cysts size and dysmenorrheal score post-treatment in observation group were lower than those in control group (P<0.05).The total efficacy in observation group was higher than that in control group (P<0.05).Conclusion Taohechengqitang combined with Danazol could reduce the CA125, E2 and P levels, relieve symptoms of dysmenorrhea and reduce the cyst volume, whose efficacy is exact.

This study was aimed to find out the relationship between the serum total cholesterol ( TC ) and coronary heart disease ( CHD ) of phlegm and blood stasis syndrome . Meta -analysis was used on the comprehensive analysis of ten research literatures on TC content of CHD patients with phlegm-turbid syndrome, blood-stasis syndrome and normal control . The results showed that the content of TC was 0.99-2.426 times h igher standard deviation in the blood-stasis syndrome patients than in the normal control, Totalcombined = 0.71. And the 95% confidence intervals of Totalcombined was 0.16-1.25. And the content of TC was 1.019-3.761 times higher standard deviation in the phlegm-turbid syndrome than in the normal control, Totalcombined= 1 . 05 . And the 95% confidence intervals of Totalcombined was 0 . 43-1 . 68 . It was concluded that there were significant variations of TC content in CHD patients with blood-stasis and phlegm-turbid syndrome compared to normal control . Therefore , abnormal changes of TC content can be used as one of the objective CHD diagnostic criteria of phlegm and blood stasis syndrome .

Objective In order to explore the ways of reflecting the dose distribution in the implementation of the of IMRT (intensity modulated radiation therapy),a 2D diode array (2D-DA) was used in verifying the composite dose distribution of IMRT plans in the way of multi-gantry-angle composite (MGAC).Methods IMRT quality assure (QA) plans of 27 patients,based on the 2D-DA and solid water phantom,were designed and verified in two ways of single-gantry-angle composite (SGAC) and MGAC verifications.The comparison and analyzation of the dose distributions of the TPS calculation and the measurement of the 2D-DA were done.Results (1) When the beam central axes were not superposed with the detectors'plane of the 2D-DA,the verification passrate of SGAC and MGAC planar dose distribution of 27 patients'IMRT plan were 94.56%±4.28% and 94.81%±3.80% (the criteria:rvalue,3 ram/3%),respectively.There was no statistical difference between the results of two sets (t =-0.213,P＞0.05).(2) When one of the beam central axes was superposed with the detectors'plane of the 2D-DA,the verification passrate of MGAC planar dose distribution were 79.72%±12.77%.Conclusions Using the 2D-DA with a proper phantom,there was no statistical difference in the SGAC and MGAC verifications of IMRT plans when the beam central axes were not superposed with the detectors'plane.However,the MGAC dose distribution can provide more about the clinical dosimetry,and the errors in the implementation of the of IMRT were easier located.