Objective To evaluate the clinical usefulness of 99m Tc MIBI imaging for the diagnosis of breast malignancy.Methods 195 patients with a total of 204 breast lesions were divided into three groups to undergo 99m Tc MIBI imaging and the results were compared with pathology.20 cases with normal breast served as control.Abnormal density showed by 99m Tc MIBI imaging in the breast(10% higher than surrounding structure) was regarded as positive.Results Of 100 breast cancer lesions,positive feature appeared in 92.Twelve out of 70 benign breast lesions showed positive imaging.Six out of 34 breast occult lesions were with positive imaging, among them 3 were finally found with carcinoma.No positive imaging was found in the control group.The diagnostic accuracy,sensitivity,specificity,positive predictive value,negative predictive value of 99m Tc MIBI was 92 0%,91 8%,92%,86%, and 92%,respectively.Conclusions[WT5”BZ] 99m Tc MIBI imaging has high sensitivity and accuracy in the diagnosis of breast cancer and in the differentiation between benign and malignant breast lesions.

OBJECTIVETo investigate the gene mutation frequencies and patterns of β-thalassemia (β-thal) in the minority populations of Guizhou province.

METHODSThree thousand and five hundred couples in the reproductive age were screened by using automatic hemocyte analyzer and hemoglobin autoanalyzer-variant. The diagnostic criteria for β-thal were: the mean corpuscular volume (MCV) was ≤ 82 fl, and the HbA(2) level was ≥ 3.5%. A total of 194 positive samples were detected and further identified by PCR-reverse dot blot (PCR-RDB) assay for 18 common β -thal mutations in Chinese population. Those subjects with positive phenotypes but without the 18 common β-thal mutations were subjected to DNA sequence analysis of the β-globin gene.

RESULTSOne hundred and eighty-nine samples with gene mutations were observed from the 3500 samples, with the incidence of β-thal being 5.4%. A total of 10 different β-thal mutations were identified from the 189 diagnosed samples. The five most common mutations were as the following: CD17 (43.9%), CD41-42 (38.6%), IVS-II-654(10.1%), -28 (2.6%) and CD71-72 (1.6%). In addition, a novel β-globin gene mutation (-CD53) allele was detected. One rare mutation of IntM was observed.

CONCLUSIONThe minority population in Guizhou province is of high risk of β-thal. It is recommended that more attention should be paid to detect the carriers of β-thal in the population in reproductive age by hematologic screening and common gene diagnosis in the area with high risk of β-thal.

Adult ; Base Sequence ; China ; ethnology ; DNA Mutational Analysis ; Ethnic Groups ; genetics ; Female ; Humans ; Male ; Middle Aged ; Mutation ; Young Adult ; beta-Globins ; genetics ; beta-Thalassemia ; genetics