1.A STUDY ON NUTRITION OF ULVA PERTUSA KJELLM
Xiurong SU ; Taiwu LI ; Shaojie CHANG
Chinese Journal of Marine Drugs 1994;0(01):-
In this paper five nutritive indexes of Ulva pertusa were measured in Liaoning coast. The results showed that in dry powder of Ulva pertusa, the protein was 6. 03% , crude fat was 3. 12%, carbohydrate 38. 88%, moisture content 12. 27%, ash content 39. 7, etc. In which the carbohydrate is made up of rhamnose, glucose, xylose, triose, fucose, mannose, galactose, arabinoe etc, measured with gas chromatography. There were many inorganic elements, such as calcium, magnesium, zinc, selenium. The results indicate that Ulva pertusa is rich in nutritive components and can be developed into valuable function food.
2.Important role in the establishment of quality control system for hernia and abdominal wall surgery in China
Jianxiong TANG ; Lei HUANG ; Shaojie LI
Chinese Journal of Digestive Surgery 2017;16(9):894-898
Some aspects of hernia and abdominal wall surgery in China have come up to an international advanced level,such as number and quality of operation.The latest issue of The Lancet gave a high score of 99 points on diagnosis and treatment of inguinal hernia in China,which has far outstripped the other developed countries of the world,with China in fourth place globally.The journal commented that,considering the treatment of inguinal hernia rely mainly on surgery,the reason Chinese surgeon performed well recalled for the great number of patients,surgery of huge quantity and the strong operation ability of the doctors in China.But there is still a wide gap on comprehensive strength between Chinese and international levels,and quality control standard is one of major aspects.If hernia and abdominal wall surgery wants to develop further,perfect quality control system is a crucial step.
3.CLINICAL ANALYSIS OF SEVERE ACUTE RESPIRATORY SYNDROME
Shaoli YOU ; Shaojie XIN ; Baosen LI
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
Objective To explore the clinical features of severe acute respiratory syndrome (SARS). Methods Clinical data of 70 patients with SARS were retrospectively analyzed. Results The age of the patients ranged from 10 to 74 years old (mean age 33.81?12.73 years). Severe type of SARS was diagnosed in 38 patients and 32 as common type of SARS. The most common symptoms included fever(98.57%), malaise(80.00%), cough(74.28%), shortness of breath (55.71%), headache(38.57%). The fever lasted for 7.20?4.57d. Based on the symptoms and chest X-ray examination, the disease was classified into early stage, progressive stage, and recovery stage with a mean duration of 3.19?2.32d, 8.18?4.36d, and 8.82?7.01d, respectively. It was found that the incidence of severe type of SARS was higher with the increase of age. Compared with common type of SARS, the fever in patients with severe type of SARS was more difficult to control (the mean highest fever 38.74?0.97℃, 38.02?0.14℃, respectively). The duration of fever after treatment (4.12?3.19d, 2.37?1.75d, respectively) was longer in the illness course (27.78?9.99d, 14.93?5.80d, respectively). The ALT, LDH and HBDH in patients with severe type of SARS were higher than those in patients with common type of SARS (ALT:78.95%,50.00%, respectively; LDH, HBDH:42.11%, 18.75%, respectively). Advanced age, pre-existing chronic disease, and elevation of LDH and HBDH were the factors of poor prognosis. Combination therapy was the first choice for the treatment of SARS. Empirical use of glucocorticoid was useful in preventing progression of the disease. Conclusion Although severe type of SARS had a high mortality, combination therapy could be very effective in the treatment of the majority of patients.
4.NEURON SPECIFIC ENOLASE IMMUNOHISTOCHEMICAL OBSERVATION ON THE PARALYMPHATIC NEURONS IN SUBMUCOUS NERVE PLEXUS IN THE GUT OF GUINEA PIGS
Jun LI ; Jin YANG ; Shaojie LIU
Acta Anatomica Sinica 1957;0(04):-
We have reported previously a peculiar type of neurons in the submucous nerve plexus in the intestine of guinea pigs, and named them tentatively the ′paralymphatic neurons′. In order to further study this type of neurons, the immunohistochemical observation for neuron specific enolase (NSE) reactivity of the enteric nerve plexus in guinea pigs was carried out in this investigation. We found that all the neurons in the enteric nerve plexus showed NSE positive reaction. The paralymphatic neurons located closely against to lymphatic vessels in submucous nerve plexus in small intestine and colon also exhibited same NSE positive reaction as other neurons. All the smooth muscle and connective tissue cells were NSE negative. Most of the paralymphatic neurons exhibited typical morphological features of neurons. Although some cells did not show obvious neuronal characters, but they all presented the same positive reactivity. Hence, this study provide another reliable evidence to confirm the exact neuronal nature of the paralymphatic neurons.
5.Expression of fibronectin protein levels in sinoatrial node tissues of hearts following electrocution
Guihua LI ; Xing WANG ; Debin LIU ; Zhijun LI ; Shaojie ZHANG
Journal of Regional Anatomy and Operative Surgery 2017;26(4):235-239
Objective To study the protein levels of fibronectin (Fn) in the sinoatrial node tissues of hearts caused by electric shock death ,and to provide a new theoretical basis for the diagnosis of electric shock death and the identification of death time.MethodsUsing immunohistochemical technique(S-P method) to detect protein levels of 15 cases of human sinoatrial node following electrocution(human experimental group),15 cases of human sinoatrial node with severe traumatic cerebral injury(human control group),35 cases of rabbit sinoatrial node following electrocution(rabbit experimental group ,divided into7 groups according to different sampling time: 0 h ,1 h ,3 h ,6 h ,12 h,24 h.48 h),and 35 cases of rabbit sinoatrial node with broken neck executed(rabbit control group ,divided int0 7 groups according to different sampling time :0 h,1 h.3 h,6 h.12 h.24 h,48 h).ResultsFn positive expression rate in human experimental group was 100% while it was 6.67% in the human control group,and the differences were statistically significant (P < 0.01 ).The Fn expression can be seen after electric shock immediately in the rabbit experimental group,and there was statistical difference compared with the rabbit control group (P < 0.05).Distribution of Fn expression became more and more widely after 3 hours t0 12 hours in the rabbit experimental group ,and there was significant difference compared with the rabbit control group(P < 0.01).The Fn expression began to decrease 24 hours after electrocution,ancl it still had statistical significance 48 hours after electrocution compared with the rabbit control group (P < 0.05).Conclusion Electric shocks significantly enhanced the expression of Fn protein levels in the sinoatrial node tissues , and optical density of Fn positive expression has a regularity change along with the extended time of death.These results could provide a theoretical reference basis for forensic diagnosis of electrocution and identification of death time.
6.Acellular tissue matrix mesh for inguinal herniopasty
Shaojie LI ; Jianxiong TANG ; Lei HUANG ; Zhao CAO ; Ge CHEN
Chinese Journal of General Surgery 2011;26(12):1039-1041
Objective To evaluate acellular tissue matrix mesh in inguinal hernioplasty in young male patients.Methods Clinical outcome and sexual function were evaluated on 12 young male inguinal hernia patients undergoing inguinal hernioplasty by ACTM mesh.Results The mean operation time was 62 minutes.Postoperative analgesics were needed for an average of 1.45 days (range,0 -4 days).The average postoperative hospital stay was 3.9 days,and patients were able to return to normal activity,after a mean 11.8 days.All patients were followed-up for a mean duration of 9.54 months(range,6 - 16 months).Two patients complained pain one month after surgery and the pain disappeared later without any medical treatment.There was no hernia recurrence and foreign body feelings,nor sexual and reproductive problems.Conclusions The ACTM mesh is a safe and effective material for inguinal hernioplasty,especially in young male patients.
7.The signification of p57kip2immunostaining to distinguish molar gestations from hydropic abortions
Xinqing YE ; Shaojie XU ; Yiling ZHU ; Wei GONG ; Qingrong LI
Chinese Journal of Primary Medicine and Pharmacy 2008;15(11):1792-1793
Objective To study the signification of p57kip2 immunostaining to distinguish molar gestations from hydropic abortions.Methods To observe the p57kip2 immunohistochemical expression in 58 cases of histological hydropic villi which were divided into complete mole(18 cases) ,partial mole(19 cases),hydropic abortion(11 cases)and undetermined hydropic abortion or molar gestations(10 cases) and in 3 normal placentas.Results Normal villi,partial mole and hydropic abortions show positive staining for p57kip2, which expressed in the nuclei of cytotrophoblast and villous mesenchyme,and complete moles show complete absence of staining in the cytotrophoblast and villous mesenchyme.According to the comparison of the diagnosis based on morphology and the one based on p57kip2 stai-ning,the later whose sensitivity is 96% (46/48) confirmed the earlier diagnosis in 58 cases studied and the later conformed to the earlier very well.Conclusion The immunohistochemical staining for p57kip2 is a valuable diagnostic mean to distinguish molar gestations from hydropic abortions and is a sensitive and specific marker for the diagnosis of complete mole.
8.Evaluation of left ventricular global systolic function using velocity vector imaging in patients with coronary heart disease before and after percutaneous coronary intervention therapy
Shufen GAN ; Yiqing WANG ; Jianghua CHEN ; Zhian LI ; Shaojie ZHANG
Chinese Journal of Ultrasonography 2011;20(8):656-660
Objective To analyze left ventricular (LV) global systolic function in patients with coronary artery disease(CAD) and their changes after percutaneous coronary intervention(PCI) using velocity vector imaging(VVI). Methods Eighteen healthy adults and twenty two patients with CAD were enrolled in this study. Two-dimensional dynamic images of standard apical four-chambers, two-chambers and parasternal short-axis views at the level of mitral valve and apex were obtained in VVI condition. All patients were examined 1 day before PCI, 1 week, 1 month and 3 months after PCI respectively. LV rotation degree, rotation velocity at baseline and apex were measured using the off-line syngo US workplace software and LV twist, torsion were calculated as global motion condition. Results LV ejection fraction(LVEF) and peak torsion at myocardial infarction group were lower than those at normal control and myocardial ischemia group. After PCI, LVEF were gradually improved in both myocardial infarction group and myocardial ischemia group,while changes of LV apex rotation degree and velocity, peak twist and peak torsion between two groups were opposite: those parameters of the former increased 1 week or 1 month after PCI but decreased 3 months after PCI,while those of the latter decreased 1 week after PCI but increased gradually 1 month and 3 months after PCI. Conclusions LV twist and torsion can be evaluated exactly by VVI.Compared with LVEF, LV twist and torsion can preferably reflect the different characteristic of LV global systolic function after coronary artery recanalization in myocardial infarction group and myocardial ischemia group.
9.Ciprofloxacin Combined with Amikacin to Reduce Drug-resistant Mutants of Escherichia coli
Mingtao LIU ; Enhua SUN ; Shaojie BI ; Yu LI
Chinese Journal of Nosocomiology 2009;0(17):-
OBJECTIVE To study whether the ciprofloxacin(CIP)combined with amikacin(AMK)will decrease the drug-resistant mutants of Escherichia coli(ECO)in vitro.METHODS The MIC of CIP and AMK alone was determined by agar plates dilution method,and the combined MIC by checkerboard method.The mutant prevention concentration(MPC)both alone and combined was determined by the method of agar plates dilution method,and then the value of selectiveity index(SI)(MPC/MIC)would be acquired.RESULTS The SI of two antibiotics separatedly were 16(CIP)and 32(AMK).When two antibiotics combined,if the concentration of AMK and CIP reached 2MIC could restrain the mutants.After these two antibiotics combined,the value of SI(MPCcombined/MICcombined)came to be 8(0.008/0.001)and 8(2.0/0.25).CONCLUSIONS Compared with alone,CIP and AMK combined can decrease the MPC and SI to ECO,and have the more effect to AMK.In this way,we can reduce the drug-resistant mutants.
10.The clinical analysis of hospital acquired acute renal failure
Xiaoling TANG ; Shaojie HONG ; Liaozhun QU ; Gongyu LI
Chinese Journal of Primary Medicine and Pharmacy 2005;0(02):-
Objective To expolre the clinical characteristic of hospital acquired acute renal failure(HA-ARF).Methods To retrospectively analyse the clinical datas of 103 patients who were suffered from acute renal failure in the duration of hospital stay within 5 years,which was compared with the homeochronous community-acquired acute renal failure(CA-ARF).Results In the 103 cases of HA-ARF,the incidence rate was 32.04% in the major age range from 60 to 74 years old,infection was the most important factor(33.01%),then other factors were cardial and cerebrovascular diseases(17.48%),acute hypovolemia(14.56%) and operation(9.71%);The complicating other organ nonfunction(34.95%),and the mortality rate was 39.80%,all the factors in HA-ARF were higher than that in CA-ARF.Conclusion The main factors of origin in HA-ARF are infection,cardial and cerebrovascular disease,acute hypovolemia and operation.The mortality rate of this disease is higher.Etilogical treatment promptly and dialysis regimen are the main means to rescue these patients.