Acute lymphoblastic leukemia has been divided into four immunological types with anti-pan-T, anti-CALLA, anti-HLA monoclonal antibo-dies and Es rosctte forming test and SmIg surface analysis. The results of immunolgical typing of 19 ALL cases reveal that the majority of those cases is C-ALL type and the B-ALL type appears rarely, the majority of T-ALL appears in boys.

Recentyearshavewitnessedmuchprogressinbothbasicresearchandclinicaltrialsregar-ding cancer immunotherapy with chimeric antigen receptor (CAR)-engineered T cells.CAR combine the varia-ble regions of a specific monoclonal antibody (scFv)with the CD3ζendodomain.The extracellular domain of CAR-engineered T cells directly dock to the tumor-associated antigen (TAA).When T cells bind to target anti-gens,they mediated redirected cytotoxicity and secrete a series of cytokines such as Perforin,Granzyme,Inter-feron-γ(IFN-γ)and Tumor necrosis factor-α(TNF-α),which would eventually lead to the necrosis of tumor cells.Although the antitumor response of the CAR-engineered T cells is considered as successful and surpri-sing,it should be noted that some safety issues have been observed in other several basic researches and clinical trials.This overview focuses upon the utility and safety of the CAR-engineered T cells.

The incidence of thyroid cancer is increasing recently, and differentiated thyroid cancer accounts for more than 90% of all thyroid malignancies.Although the degree of malignancy of thyroid cancer is lower and prognosis is better, but there is pos-sibility to relapse and metastasis.The concept of precision medicine has paid more and more attention by many surgeons.This paper reviews how to get perfect diagnosis and therapy of differentiated thyroid cancer to reduce risk of relapse and metastasis by application of precision medicine.

Objective To analyze the different procedures and their clinical effects on treatment for pan-creatic pseudocyst( PPC) .Methods The management and outcome of 78 patients with PPC from Jan.2002 to Jun.2012 were retrospectively analyzed.Results 20 patients underwent conservative treatment and the pseudo-cysts absorbed spontaneously during follow up.Invasive procedures were performed in 58 cases, including percu-taneous catheter drainage in 3 cases with 1 postoperative pancreatic leakage and 1 postoperative pancreatic ab-scess;PPC external drainage in 5 cases with 2 postoperative pancreatic leakage and 1 postoperative bleeding;pancreatic pseudocyst stomach anastomosis in 15 cases with 1 postoperative gastrointestinal bleeding;Roux-en-Y cystjejunostomy in 29 cases with 2 gastrointestinal bleeding;cystduodenostomy in 2 cases;pancreatic pseudocyst excision in 2 cases with 1 postoperative pancreatic leakage; and laparoscopic cystogastrostomy in 2 cases.Con-clusions At present, there are various treatment options for pancreatic pseudocyst and it is necessary to choose treatment strategy according to patient's condition.Internal drainage is still the primary option of surgical proce-dures in treatment for pancreatic pseudocyst.

Objective Recently a lot of studies have shown carbon nanoparticles can protect parathyroid, however, there is no application in outpatient thyroid operation. The article was to investigate the influence of carbon nanoparticles suspension ( CNS) in?jection on the operation and prognosis of outpatient thyroid microcarcinoma(TMC) surgery. Methods Retrospective analysis was conducted on the clinical data of 65 patients who underwent outpatient TMC surgery from January to June in 2015 in our hospital. The patients were divided into 2 groups:33 patients in trial group were injected with CNS, and the other 32 patients in control group under?went conventional surgery. Comparison was made in operation information, pathologic data, postoperative complications, postoperative hospital stay as well as C?reactive protein(CRP) and blood calcium of the first day after operation between the two groups. Results The average number of VI region lymph nodes in trial group(8.21±2.08)was significantly higher than that of control group(P=0.004). Removal of 5 parathyroid glands for mistake was detected in control group, but no removal of parathyroid gland was detected in trial group, showing significant difference (P=0.028). 3 patients in control group had numbness in skin, while none in trial group. The hospital stay of trial group was lowered significantly in comparison to that of control group([31.09±6.62] vs [35.75±7.37], P=0.009). The average C?reactive protein ( CRP ) of the first day after operation in trial group was much lower that of control group([7.17±5.20] vs [9.87±5.60] mg/L, P=0.048). The average blood calcium of the first day in trial group was much higher than that of control group ([2.07±0.17] vs [1.98±0.20] mmol/L, P=0.036). Conclusion Outpatient TMC surgery injected with carbon nanoparticles sus?pension can protect parathyroid gland function, reduce surgical stress and accelerate recovery.

Scutellarin (SC),a botanical extract,possesses a variety of suppressing free radical generation,anti-inflammatory,cardioprotection and anti-virus activity.Recently,many studies show that SC has inhibitory effects on the growth of many tumors and is a kind of potential anti-tumor drug.Its anti-tumor mechanisms mainly include inducing apoptosis,arresting cell cycle,enhancing the adhesion between cells,inhibiting tumor invasion,reversing multidrug resistance,anti-angiogenesis and so on.

The phosphatidylinositide-3-kinase(PI3K)/ protein kinase B(Akt)/ mammalian target of rapamycin(mTOR)pathway is recognized to have an important role in the development and progression of colo-rectal cancer(CRC). The most extensively characterized downstream targets of mTORC1 are ribosomal protein S6 kinase 1(p70S6K1)and eukaryotic translation initiation factor(eIF)4E-binding protein 1(4EBP1),both of which are crucial to the regulation of protein synthesis. The abnormal expression of p70S6K1 and 4EBP1 in CRC has become the focus of attention.

The inhibition of bronchoalveolar surfactant function by endotoxic rat plasma was compared with that by normal rat plasma with M-8601 film balance. The results showed that endotoxic rat plasma significantly inhibited surfactant function of bronchoalveolar lavage (BAL). In the same protein concentration, the inhibition effects caused by endotoxic rat plasma were comparable with those by normal rat plasma. There were no changes of the inhibition effects after incubation of BAL with either endotoxic or normal plasma at 37℃ for 30min. These results suggest that the inhibition of surfactant function by the leakage of plasma in endotoxic lung injury is mainly due to protein components.

ObjectiveTo study the effect and clinical meaning and pathogenic of extinction on cerebral cortex lesion. MethodsTactile extinction to classical extinction test(simple stimuli)and modified Quality extinction test(QET)were used. ResultsThe positive rate of ex-tinction using modified QET was higher than that of simple stimuli; left cortex lesion could result in extinction both in the contralateral side andthe ipsilateral side;left frontal sites lesion caused ipsilateral extinction. Conclusion: The modified QET was more sensitive than simplestimuli; modified QET showed extinction has competiting and restricting effect at 2 different levels, the mechanism of extinction is correlatedto competition and adventage restraint of diplo-hand cerebral hemisphere and liaise correliation fiber of diplo-hand cerebral hemisphere; thehighesttthe positive rate of extinction was observed in left parietal lesions.

Objective To explore the temporal expressions of interleukin-10(IL-10)and interleukin-4(IL-4)and their relationship with the wound age during skin incised wound healing in mice.Methods Immunohistochemical and image-analysis techniques were employed in vital skin wounds 0.5h～168h after incision and postmortem incision 0.5h～6h after death.Results Weak expression of IL-10 was detected at 0.5h after incision,which increased subsequently,and peaked at 3h post-injury.Then decreased to the pre-incision level at 24h,but peaked again at 72h after injury.Immunostaining revealed that epidermal cells and infiltrating mononuclear cells were the major source of IL-10.IL-4-positive cells were observed in the dermis at 24h,and reached the maximum level at 96h,which maintained until 168h post-injury.IL-4 was mainly expressed in the fibroblast-like cells of the granulation tissue and dermis.In addition,faint IL-10-positive cells were merely identified in the epidermal cells in the postmortem incision skin within 1～3h after incision.No IL-4-postitive cells were found in the postmortem incision skin.Conclusion The time-dependent expressions of IL-10 and IL-4 during cutaneous wound healing may be used for the estimation of wound age.