Objective To analyze the different procedures and their clinical effects on treatment for pan-creatic pseudocyst( PPC) .Methods The management and outcome of 78 patients with PPC from Jan.2002 to Jun.2012 were retrospectively analyzed.Results 20 patients underwent conservative treatment and the pseudo-cysts absorbed spontaneously during follow up.Invasive procedures were performed in 58 cases, including percu-taneous catheter drainage in 3 cases with 1 postoperative pancreatic leakage and 1 postoperative pancreatic ab-scess;PPC external drainage in 5 cases with 2 postoperative pancreatic leakage and 1 postoperative bleeding;pancreatic pseudocyst stomach anastomosis in 15 cases with 1 postoperative gastrointestinal bleeding;Roux-en-Y cystjejunostomy in 29 cases with 2 gastrointestinal bleeding;cystduodenostomy in 2 cases;pancreatic pseudocyst excision in 2 cases with 1 postoperative pancreatic leakage; and laparoscopic cystogastrostomy in 2 cases.Con-clusions At present, there are various treatment options for pancreatic pseudocyst and it is necessary to choose treatment strategy according to patient's condition.Internal drainage is still the primary option of surgical proce-dures in treatment for pancreatic pseudocyst.

Forensic medical talents with creative ability should have ability to find.analyze and solve problems from complex cases.According to teaching practice on the basis of PBL this article illustrates application methods of PBL teaching characters in the training of forensic medical talents with creative ability.

[Objective] To observe the effect of Pueraria Lobata isoflavones (PLI) on neuropeptide-Y-induced (NPY) vascular smooth muscle cell (VSMC) proliferation. [Methods] Experiment system for VSMC was set up as 4 groups: control group (with DME culture fluid, free of blood serum; group A), model group (with NPY culture fluid; group B), control + PLI group (with PLI culture fluid; group C) and model + PLI group (with NPY and PLI culture fluid; group E). Quantitative fluoroimmunohistochemistry techniques were applied to examine the mean fluorescent values of proliferating cell nuclear antigen (PCNA), platelet derived growth factor (PDGF) and C-myc expression. [Results] The levels of PCNA, PDGF and C-myc expression in model group were higher than control group (P

Objectives:To evaluate the effectiveness of small fly spot scan excimer laser in situ keratomileusis for treatment of myopia accompanied with moderate astigmatism. Methods:Ninety six cases and 145 eyes were divided into three groups according to the preoperative refractive diopters. GroupⅠ: myopia 0～-6 D, astigmatism -2～-4 DC, groupⅡ:myopia -6.25～-10.0 D, astigmatism -2～-4 DC, and group Ⅲ:myopia≥10.25 D, astigmatism -2～-4 DC. The postoperative follow up was more than six months. Results:One month after the operation, uncorrected visual acuity equal to or better than 0.6 were 5.3% in groupⅠ, 15.8% in groupⅡ, and 66.7% in group Ⅲ. Six months after the operation, uncorrected visual acuity equal to or better than 0.6 were 4% in group Ⅰ,14% in groupⅡ,and 75% in group Ⅲ.Uncorrected visual acuity equal to or better than 1.0 were 96% in group Ⅰ, 86% in groupⅡ, and 25% in group Ⅲ.Postoperative refractive error within ?1.00 D were 8% in groupⅠ,14% in groupⅡ, and 41.7% in group Ⅲ. Postoperative refractive error within ?0.50 D were 92% in groupⅠ, 86% in groupⅡ, and 58.3% in group Ⅲ. Postoperative refractive clinder within ?1.00 DC were 8% in groupⅠ,10.5%in groupⅡ, and 50% in group Ⅲ. Postoperative refractive clinder within ?0.50 DC were 92% in group Ⅰ,89.5% in groupⅡ, and 50% in group Ⅲ. The average exciter zone diameter was (8.02?0.069)mm. Conclusions:Small fly spot scan exciter laser in situ keratomileusis is an effective and safe method to treat mild and moderate myopia accompanied with moderate astigmatism.

Objective To explore the effect of myocardial cell Oil high bilirubin blood newborns.Methods Total bilirubin and myocardial ferment value(AST,LDH,CK,CK-MB)in high bilirubin blood group(n=104)and control group(n=60)were examined at the game time.Once again examined total bilirubin and myocardial ferment value after synthetical treat the hish bilirubin blood group.Results The myocardial ferment value diversity significant (P＜0.01) in bilirubin blood group and control group;mild group and severe group myocardial ferment value dlversity notable(P＜0.01),especially CK-MB.Normal value approach of myocardial ferment with bilirubin value after treat the billrubin blood group.Conclusion Myocardial cell damage is temporary and a period of time can bring about of hish bilirubin blood newborns.

Objective To discuss the health education modes in order to promote the effect of health education for TURP(transurethral resection of prostate)patients and their family members.Methods A total of 380 cases after TURP were divided into the observation group and the control group at random with 190 cases in each group.The discharge instruction for the patients and their family members in the observation group was in the form of health education prescription,while the discharge instruction for the control group was in the form of oral instruction.The awareness rate of disease-related knowledge and incidence of complication were compared between the two groups. Results The awareness rate of disease-related knowledge in the observation group was higher,and incidence of complications was lower than that of the control group.Conclusions The application of health education prescription in the discharge instruction for the patients after TURP can guarantee the effectiveness of health education,simultaneously decrease the incurrence of complications and improve the life quality of patients.

Objective To explore the influence of preoperative chemotherapy (POCT) on telomerase activity(TA) of breast cancer and its mechanism. Methods TA was detected in 40 primary breast cancer specimens. Forty patients were divided into two groups: (1)POCT group. 32 patients, who underwent POCT using CMF scheme; (2) control group . 8 patients, who did not receive any POCT or radiotherapy. Results TA in POCT group(TA=0.499) was significantly lower than that in control group (TA=1.018). TA in patients received one , two or three times of POCT was 0.812,0.492 and 0.280 respectively. The difference of TA in patients received 1 time POCT and receive ≥ 2 times POCT was significant (P

AIM:To investigate the interplay between the neuropeptide Y(NPY) and renin-angiotensin system, and relationship to the pathogenesis of hypertension. METHODS: The method of cellular culture, MTT colorimetric assay and quantitative immunocytochemistry through ACAS570 were performed for the effect of neuropeptide Y on proliferation of cultured rat vascular smooth muscle cell (VSMC) and losartan treatment. RESULTS:It was observed that exposure of VSMC to neuropeptide Y could stimulate the proliferation of VSMC and caused increase respectively in MTT OD values and expression of proliferating cell nulear antigen(PCNA) but losartan interfered with NPY stimulating effects on VSMC and decreased MTT OD values and expression of PCNA. CONCLUSION:These results demonstrated that the NPY could promote proliferation of VSMC, this effect was partly preformed through angiotensin Ⅱ receptor.

AIM: To observe expression of proliferating cell nuclear antigen (PCNA), platelet derived growth factor (PDGF) and c-myc in cultured vascular smooth muscle cells which is effected by neuropeptide Y (NPY) and losartan (the receptor blocking agent of angiotensin Ⅱ) therefore exploring effects of NPY on the generation of hypertension and its relationship with losartan reverse treatment in molecular biology. METHODS: Applied the method of quantitative immunocytochemistry through ACAS570. RESULTS: 24 hours exposure of vascular smooth muscle cell to NPY caused an increase in expression of PCNA, PDGF and c-myc respectively. But losartan could reverse these expressions by NPY, decreased the expression of PCNA, PDGF and c-myc. CONCLUSION: NPY is closely related to the generation of hypertension. But losartan could reverse these effects of NPY.

Objective Aims To analyze the etiology of intestinal nutrition related diarrhea and to study its nursing. Method The rate and etiology of intestinal nutrition related diarrhea of 180 patients receiving intestinal nutrition through traditional nasal way from 2002 to 2003 year 180 patients(A group) was analysed retrospectively and new nursing was studied.From 2004 to 2005 year,202 patients(B group) received intestinal nutrition through nutrition pump and intensive nursing.The intestinal nutrition related diarrhea rate of two groups was compared. Results The diarrhea rate of B group was insignificantly lower than that of A group(P