Cell Transformation, Neoplastic ; metabolism ; Humans ; Liver Neoplasms ; metabolism ; Non-alcoholic Fatty Liver Disease ; metabolism ; PPAR alpha ; metabolism

At present, more and more studies show that epithelial-mesenchymal transition (EMT) is closely associated with cancer stem cell formation and tumor cell invasion, migration and metastasis.EMT not only enhances the capacity of cancer cell invasion and metastasis, but also makes the cell obtain self regeneration and other stem cell characteristics and promotes the production of cancer stem cells.As a result, the investigation of the relationship between EMT and common stem cells can be served as a new approach for searching for effective ways to control tumor invasion and metastasis.It also can provide new targets for the treatment of tumor.

Objective　To study the effect of the combined use of aminoguanidine (AG) and cyclosporine A (CsA) on acute rejection following cardiac allograft. Methods　Except the control group, all other three groups were respectively treated by AG 600mg/kg every day subcutaneously 0 to 7 days after transplantation, low-dose CsA 2mg/kg every day i.m 0 to 7 days after transplantation, and low-dose CsA plus AG. Inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production during acute allograft rejection were detected at 4th day after operation, and the graft survival was observed.Results　Compared with the control group, iNOS expression and NO production were significantly inhibited in the AG-treated group (P＜0.05)，the progression of acute rejection was slightly attenuated and allografted heart survival prolonged. The inhibitory effects of iNOS activity and NO production in the recipients receiving the combined treatment of low-dose CsA plus AG were higher than those receiving low-dose CsA alone. Compared with the low-dose CsA group, low-dose CsA plus AG could significantly reduce the histologic grade of acute rejection (P＜0.01) and prolong the graft survival (P＜0.05). Conclusion　The combined use of low-dose CsA and AG could synergistically suppress iNOS activity and NO production, and significantly prolong allografted heart survival.

Objective To explore the effect of evidence-based care on the constipation of psychiatric patients.Methods The constipations in 328 psychiatric patients admitted to our department were intervened with evidence-based nursing.Frequency for defecation,incidence of difficult defecation and incidence of dry stool before intervention were compared to those two weeks after intervention.Result Two weeks after intervention,the defecation frequency,incidence of difficult defecation and incidence of dry stool were all significantly lower than those before the intervention(P<0.001).Conclusions The evidence-based nursing used in care to psychiatric patients with constipation can reduce the incidence of constipation,and relieve their physiological and psychological pains.

Forensic medical talents with creative ability should have ability to find.analyze and solve problems from complex cases.According to teaching practice on the basis of PBL this article illustrates application methods of PBL teaching characters in the training of forensic medical talents with creative ability.

BACKGROUND:Due to anisotropic CT volume data, triangular meshes extracted from bone CT images often contain staircase surface, which wil affect the subsequent medical diagnosis. OBJECTIVE:To reconstruct patient-specific bone model based on digital geometry processing technique. METHODS:Firstly, registration was performed in image registration algorithm based on mutual information for bone CT slices, and then contours were extracted by image segmentation and a stack of contours were converted into point clouds. The normals of point clouds were estimated based on Gaussian weighted principal component analysis and the noise from point clouds was removed by trilateral filtering. Final y, bone triangular meshes were constructed by adaptive spherical cover. RESULTS AND CONCLUSION:In this paper, the proposed method could generate smoothing bone surface meshes, triangular mesh shape which was formed by the rules and adaptive distribution, for finite element analysis, computer aided manufacturing and three-dimensional printing to provide accurate three-dimensional models.

Objective To understand the cooperation experiences of doctors and nurses from the Infectious Department,that may be contributive to promote a harmonious relationship between doctors and nurses.Methods Sixteen nurses and nine doctors came from Infectious Department were interviewed.The data were collected through face-to-face in-depth semi-structured interviews.Thematic analysis based on Qualitative Research method,qualitative content analysis was used for data interpretation by analysis and management.Results Three themes were identified and described as the cooperation experiences between doctors and nurse,the cooperation troubles,suggestions about promoting a harmonious relationship between doctors and nurses.Conclusion Harmonious relationship is particularly important in the clinical work,but in fact,there are many factors affect the harmonious relationships,so it is recom-mended that hospital leaders should take measures to build a harmonious relationship between doctors and nurses.

T cell immune deficiency results in inefficient anti-tumor immune response in most patients with hematological malignancies,thereby tumor cells escaping the attack of host immune system,that is the immune escape of tumor cells.Recently,new insights regard to the CD8+ T cell exhaustion which is one of the most important mechanisms of tumor immune suppression.The CD8 + T cell exhaustion is mediated by abnormal expression of T-cell immunosuppressive receptors such as PD-1 and CTLA-4.Blocking these molecules may restore the partial or all functions of T cells.This article reviewed the advances on the role of the PD-1 and CTLA-4 in hematological malignancies,including their functions on mediating T cell-immune tolerance and the progression of targeted immunotherapy,to provide the new strategy for hematological malignancies.

Objective To explore the effect of myocardial cell Oil high bilirubin blood newborns.Methods Total bilirubin and myocardial ferment value(AST,LDH,CK,CK-MB)in high bilirubin blood group(n=104)and control group(n=60)were examined at the game time.Once again examined total bilirubin and myocardial ferment value after synthetical treat the hish bilirubin blood group.Results The myocardial ferment value diversity significant (P＜0.01) in bilirubin blood group and control group;mild group and severe group myocardial ferment value dlversity notable(P＜0.01),especially CK-MB.Normal value approach of myocardial ferment with bilirubin value after treat the billrubin blood group.Conclusion Myocardial cell damage is temporary and a period of time can bring about of hish bilirubin blood newborns.

ObjectiveTo study the correlation between human erythrocyte membrane phospholipid profile and insulin sensitivity in elderly diabetic patients. MethodsThe levels of phosphatidylcholine (PC), phosphatidylserine(PS),phosphatidtlinostiol(PI),phosphatidylethanolamine(PE),the maximum erythrocyte deformation index(DImax) , the maximum erythrocyte aggregation index(AImax) and glucose disposal contant Ki (KITT) were measured in 32 cases of elderly diabetics and 30 healthy old subjects. ResultsThe KITT was lower in diabetic patients than in normal controls (P