The effect of endotoxin on serum activity of angiotensin converting enzyme (ACE)in dogs was studied.At a dose of 2 mg/kg intravenously administered, endotoxin from Serratia marcescens increased serum ACE.Histological examination demonstrated severe injuries of the lung and liver.The changes in serum ACE may be due to the injuries of the lung as well as the liver.

We observed the dynamic changes in angiotensin-converting enzyme in serum, pulmonary lymph and lung tissue after acute hemorrhagic pancreatitis induced by injection of trypsin and autobile into the pancreatic duct in dogs, Pulmonary lymph was collected for assessment of the changes in transvascular fluid and protein exchanges. The ACE activity in the mixed venous blood decreased 15 min after injection. The ACE activity in the arterial blood increased 30 rain after injection and declined 45 min later. The ACE activity in pulmonary lymph increased steadily after injection, but in the lung tissue of the pancreatitis dogs it markedly decreased comparing with that of the controls. The pulmonary lymph flow and transvascular protein clearance increased 2 to 3-fold which paralleled with the changes in ACE activity in serum and pulmonary lymph. The results indicate that the changes in ACE activity reflected the impairment of pulmonary vascular endothelial cells and could be used for the diagnosis of acute lung injury.

Cell Transformation, Neoplastic ; metabolism ; Humans ; Liver Neoplasms ; metabolism ; Non-alcoholic Fatty Liver Disease ; metabolism ; PPAR alpha ; metabolism

Objective: To explore the operation method and clinical effect of treatment for bilateral cleft lip with improved straight line closure. Methods: On the basis of straight line closure, the vermilion and anterior lip pear were repaired with the muscle-vermilion labial flap of lateral lip. In order to close nasal floor, flap-C from lateral wall of anterior lip and flap-G from nasal wing base were designed, meanwhile, nasal mucosa flap-D and flap-H from two sides of nasal base fissure were designed. The function of the orbicularis oris was repaired by cutting off the anomaly adhesion of the orbicularis oris. Results: The post-operational upper lip was thickened together with good shape and vermilion color, there was no obvious dynamic abnormality, nasal floor was closed well. Conclusion: The improved straight line closure can obtain good shape of lip, reduce the whistling deformities, oronasal fistula and dynamic abnormality.

Objective To study the effects of direct-current field stimulation on spinal cord motor neurons apoptosis after sciatic nerves transection. Methods In experimental group direct-current stimula tors were put into the relevant spinal cord segments and electric stimulation were given instantly after sciatic nervet ransection.In control group, the same st I mulator were put inside without courrent output.The spinal cord specimens were taken 1，4，7，14，28 days after operation. The morphology of the spinal cord motor neurons was observed with the methods of HE staining and light microscope.The spinal cord motor neurons were counted and analyzed with three-dimensional quantit ative method.The TUNEL staining of the specimens was performed simultaneously and the positive-stain spinal cord motor ne u rons were counted. Results 4，7，1 4 and 28 days af ter operation, the number of spinal cord motor neurons and the size of the neurons increased obviously with compare to the control group.The TUNEL staining positive spinalcord motor neurons decreased sign I ficantly according to the control group. Conclusion Direct-current stimu lation may have intervention effects on spinal cord neuron apoptosis after sciatic nerve transection.

E-Cadherin is one of the Ca2+-dependent cell adhesion molecule family.It plays an important role in maintaining the normal morphology and structural integrity of epithelial cells.This review aims to clarify the structure,function and regulation etc.of E-Cadherin.

Objective To investigate the effect of intramedullary stabilization of tibiofibular fractures with titanium elastic nails (TENs) in children .Methods Totally 59 children patients with tibiofibular fractures ,from January 2008 to November 2013 ,were stabilized with TENs in the C‐arm fluoroscopy in our hospital .All patients were followed up after surgery ,including fracture heal‐ing ,wound conditions ,limb length ,range of motion of knee and ankle ,gait ,etc .Results All of the 59 cases were followed up for 6-36 months ,average 16 months .All fractures were healed in good alignment .There were no postoperative complications such as infections ,swelling and pain at the end of the nails ,TENs broken ,tibial epiphysis injuries and unnormal function of the lower limb joint .According to the Flynn evaluation standard ,excellent result were observed in 49 cases ,good in 10 cases ,the excellent and good rate was 100% .Conclusion Intramedullary stabilization with TENs can achieve effective fixation in the treatment of tibiofibular fractures ,it has advantages of micro‐trauma ,tiny tissue damage ,little influence on blood circulation at the fracture site ,good stabili‐ty ,rapid healing ,short hospital stay and few complications .

The inhibition of bronchoalveolar surfactant function by endotoxic rat plasma was compared with that by normal rat plasma with M-8601 film balance. The results showed that endotoxic rat plasma significantly inhibited surfactant function of bronchoalveolar lavage (BAL). In the same protein concentration, the inhibition effects caused by endotoxic rat plasma were comparable with those by normal rat plasma. There were no changes of the inhibition effects after incubation of BAL with either endotoxic or normal plasma at 37℃ for 30min. These results suggest that the inhibition of surfactant function by the leakage of plasma in endotoxic lung injury is mainly due to protein components.

Acquired immune deficiency syndrome (AIDS), a great epidemic t hreatening disease, is caused by the human immunodeficiency virus (HIV), particu larly the strain known as HIV-1. Nevirapine, a novel non-nucleoside reverse tr anscriptase (RT) inhibitor combined with nucleoside analogue RT inhibitors or pr otease inhibitors, can be used in treatment of patients with HIV infection. AS a n agent used alone, nevirapine can prevent vertical transmission or HIV infectio n.

Platelet-activating factor(PAF) at different concentrations were added to in vitro cultured bovine=pulmonary artery endothelial cells(BPAEC) when lactate dehydrogenase(LDH) release rate, angiotensin converting enzyme(ACE) activity and malondialdehyde (MDA) content were determined. The influences of specific PAF receptor antagonist on PAF effects and the stimulating effect of PAF on leukocyte-endothelial cell adhesion were also observed. The results showed that when PAF were added to BPAECs, there were no significant change in LDH release rate, ACE activities were only slightly increased. No obvious changes in cellbound MDA, but supernatant MDA content was increased in cells treated with high concentration of PAF (10~(-6)). The PAF receptor antagonist, SRI 63-441 made the cell-bound MDA content higher than that of PAF treated cells, whereas the supernatant MDA became lower. PAF may promote endothelial-leukocyte adhesion either through its effects on endothelial cells or on leukocytes, suggesting that PAF has no obvious damaging effect on endothelial cells but may activate endothelial cells thus promoting endothelialleukocyte adhesion.