1.Determination of 17 Characteristic Ingredients of Plant Extracts in Hair Growth Cosmetics by Ultra High Performance Liquid Chromatography
Jianhua TAN ; Huiyong LI ; Shaofeng XI ; Changhong GUO ; Jicai WANG ; Xiaoting XIONG ; Yanping XIAN ; Xindong GUO
Chinese Journal of Analytical Chemistry 2015;(1):110-114
A method was developed for the simultaneous determination of 17 characteristic ingredients of plant extracts, including paeoniflorin, hydroxysafflor yellow A, calycosin_7_glucoside ferulic acid, etc. , in hair growth cosmetics using ultra high performance liquid chromatography ( UPLC ) . Different cosmetic samples were extracted by ultrasonic_assisted extraction with the solvent of methanol/water (4∶1, V/V) solution. After demulsified by the addition of appropriate amount of NaCl and high speed centrifugation, the supernatant was transferred and analyzed with UPLC. The separation was conducted on a Waters reversed phase column of ACQUITY UPLC CSH C18(50 mm×2. 1 mm, 1. 7μm), and the mobile phases were methanol and the solution of 0. 05% phosphate in water. The detection was performed with a photodiode_array ( PDA) detector. The linear range was 0 . 2-25 mg/L with correlation coefficients higher than 0 . 999 . The limits of detection were within 0. 3-1. 5 mg/kg, and the limits of quantification were from 1. 0 to 4. 0 mg/kg. The average recoveries of 17 characteristic ingredients were within 93 . 5%-105 . 0%, with the intra_and inter_day precision ( n=6 ) less than 4. 6%. This method was simple, rapid, with good_repeatability, and had been applied to the analysis of real samples.
2.Abnormal Metabolic Connectivity in Rats at the Acute Stage of Ischemic Stroke.
Shengxiang LIANG ; Xiaofeng JIANG ; Qingqing ZHANG ; Shaofeng DUAN ; Tianhao ZHANG ; Qi HUANG ; Xi SUN ; Hua LIU ; Jie DONG ; Weilin LIU ; Jing TAO ; Shujun ZHAO ; Binbin NIE ; Lidian CHEN ; Baoci SHAN
Neuroscience Bulletin 2018;34(5):715-724
Stroke at the acute stage is a major cause of disability in adults, and is associated with dysfunction of brain networks. However, the mechanisms underlying changes in brain connectivity in stroke are far from fully elucidated. In the present study, we investigated brain metabolism and metabolic connectivity in a rat ischemic stroke model of middle cerebral artery occlusion (MCAO) at the acute stage using F-fluorodeoxyglucose positron emission tomography. Voxel-wise analysis showed decreased metabolism mainly in the ipsilesional hemisphere, and increased metabolism mainly in the contralesional cerebellum. We used further metabolic connectivity analysis to explore the brain metabolic network in MCAO. Compared to sham controls, rats with MCAO showed most significantly reduced nodal and local efficiency in the ipsilesional striatum. In addition, the MCAO group showed decreased metabolic central connection of the ipsilesional striatum with the ipsilesional cerebellum, ipsilesional hippocampus, and bilateral hypothalamus. Taken together, the present study demonstrated abnormal metabolic connectivity in rats at the acute stage of ischemic stroke, which might provide insight into clinical research.
Acute Disease
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Animals
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Brain
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diagnostic imaging
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metabolism
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Brain Mapping
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Disease Models, Animal
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Fluorodeoxyglucose F18
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Glucose
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metabolism
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Infarction, Middle Cerebral Artery
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diagnostic imaging
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metabolism
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Male
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Neural Pathways
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diagnostic imaging
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metabolism
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Positron-Emission Tomography
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Radiopharmaceuticals
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Random Allocation
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Rats, Sprague-Dawley