Objective To investigate the effects and prognosis of aspirin combined with atorvastatin calcium for the treatment of acute cerebral infarction.Methods According to the digital table,80 patients with acute cerebral infarction were selected and randomly divided into the control group and observation group,each group 40 cases.The patients in control group were given aspirin enteric-coated metformin hydrochloride 100mg/d treatment,while the ob-servation group was treated in the control group taking on the basis of atorvastatin calcium 20mg/d.After the continu-ous treatment of 4 weeks,the curative effects were compared between the two groups,and the different hs-CRP levels in the body of the prognosis of patients were analyzed.Results The total effective rate of the observation group was 95%,which was higher than 75%of the control group(χ2 =3.98,P<0.05);The total curative effect of patients with abnormal hs-CRP levels in the control group was 65%,while that in the observation group was 92%,there was statis-tically significant diference between the two groups(χ2 =4.21,P<0.05);The total curative effect of patients with normal hs-CRP levels in the control group was 94%,while that in the observation group was 100%,there was no sta-tistically significant difference between the two groups(χ2 =1.32,P>0.05).Conclusion Aspirin combination with atorvastatin calcium for the treatment of acute cerebral infarction exactly can well improve the prognosis,which is wor-thy of clinical popularization and application.

ObjectiveTo investigate the expression of pro inflammatory cytokine mRNA including interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor-α(TNF-α) after spinal cord injury (SCI), and the effect of methylprednisolone (MP) on the expression of the cytokine and neurological outcome after SCI in rats.MethodsA moderate SCI model was made with SD rats according to modified Allen's method. 56 SD rats were divided randomly into the sham group (n=4), SCI group (n=24), saline group (n=14) and MP group (n=14, MP 30 mg/kg injected throuh tail vein 30 min after injury). Samples of injured spinal cord were taken at 1 h, 4 h, 12 h, 24 h, 72 h and 7 day after SCI. The mRNA expressions of IL-1β,IL-6 and TNF-α in the spinal cord of rats were measured by semi-quantitative RT-PCR at 1 h, 4 h, 12 h, 24 h, 72 h and 7 d post-injury, and 4 h post-operation in the control group. The effect of MP (30 mg/kg, iv, within 30 minutes post-injury) on the expression of cytokine at 4 h post-injury was tested with RT-PCR. The neurological outcome of rats was evaluated with BBB scores.ResultsThe expression of the cytokine mRNA was little in the sham group and significantly increased in injured groups with IL-1β at 12 h, TNF-αat 4 h reaching peak after injury,and higher than that in sham group until 72 h (P<0.05). While, the expressions of IL-1β and TNF -α mRNA were inhibited obviously in MP group at 4 h after injury, but MP had no effect on the neurological outcomes.ConclusionMP can inhibit inflammatory reaction obviously.

The early dynamic 18 F?FDG PET/CT can reflect perfusion, bio?distribution, uptake and excretion of 18 F?FDG in certain organs ( or lesions) in the early phase post injection. This imaging technique could be used to evaluate the organ ( or lesion) perfusion and to serve as a supplement for conventional 18 F?FDG PET/CT in the evaluation of glucose metabolism. This review summarizes the basic principle, imaging methods and clinical application of early dynamic 18 F?FDG PET/CT.

Objective To obtain the molecular epidemiological data of Human Papilloma virus(HPV) 16 and HPV 18 and screen new HPV strains. Methods Common type HPV, HPV16 and HPV18 were amplified by PCR and then cloned and sequenced. Results The infection rates of HPV16、 HPV18 accounted for 10.51% of total HPV infection rate in the area. The infection rate of HPV16 was higher than that of HPV18 ( P

OBJECTIVE: To prepare Kuijieyu enemas and establish its quality standard.METHODS: Most of traditional Chinese medicines in prescription were decocted using water and some were crushed.Then they were concentrated to prepare enemas.Pueraria lobata,Rhizoma Coptidis,Scutellaria baicalensis in Kuijieyu enemas were qualitatively identified by TLC.The content of baicalin in Kuijieyu enemas was determined by HPLC.RESULTS: The TLC spots were clear,well-separated and specific.The linear range of baicalin were 210～1 750 ?g(r=0.999 9) with an average recovery of 99.13% (RSD=1.1%,n=6).CONCLUSION: The preparation process is simple and convenient.The quality control method is simple,reliable,specific and reproducible.

Objective\ To observe the long term results of expansive open door laminoplasty of cervical spine for cervical spinal stenotic myelopathy.Methods\ From April 1986 to March 1988, 98 cases were operated upon with expansive open door laminoplasty of cervical spine. Sixty one cases had been followed up for an average of 11 years and 8 months. The long term results were analyzed.Results\ The best results were obtained at the third year after operation among the OPLL (32 cases),DCS (20 cases) and CSM (8 cases),with an average JOA recovery rate for an average of 72.30%in OPLL, 67.74%in DCS and 67.80%in CSM. JOA recovery rate were 66.81%in OPLL, 66.57%in DCS and 66.1%in CSM at following up for an average of 11 years and 8 months. Instability of the cervical spine and reclosed open door were not found at the follow up for an average of 11 years and 8 months.Conclusion\ The long term results of expansive open door laminoplasty of cervical spine for cervical spinal myelopathy were good and stable. Expansive open door laminoplasty of cervical spine did not affect the stability of cervical spine.\;

Objectives To prepare the gastric adenocarcinoma related cDNA microarray and to screen differentially expressed genes between human gastric adenocarcinoma and normal gastric mucosa. Methods A set of cDNA microarray slides containing a duplicate set of 576 cDNA spots. These selected clones have been verified in sequence. The PCR products of human genes were spotted onto a chemical-material-coated glass plates in array. DNAs were fixed on the glass plate after series of treatment. Both RNAs from the gastric adenocarcinoma and normal gastric mucosa were reversely transcribed to the cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for fluorescent signals to display differences between two tissues. Results Gastric adenocarcinoma related cDNA microarray slides contained 111 differentially expressed genes of gastric adenocarcinoma, 37 known genes related to gastric carcinogenesis (such as p21ras, p53, etc.) and control genes. The results of 10 couples of hybridizations to the gastric adenocarcinoma related cDNA microarray slides with Cy3-labeled cDNA probe from normal gastric tissue and Cy5-labeled cDNA probe from gastric adenocarcinoma showed analyzed spots uniformity background and clear signal. Among the 576 target genes, the expression level of 48 genes differed between gastric adenocarcinoma and normal mucosa in 5 or more samples (eight genes differed in all samples). There were 39 down-regulated and 9 up-regulated genes in gastric adenocarcinoma. Conclusion Gastric adenocarcinoma related cDNA microarray slides were successfully developed. The results of the experiments showed that gastric adenocarcinoma related cDNA microarray slides are reliable, sensitive and reproducible.

Objective To evaluated the clinical outcomes of intravesical instillation resiniferatoxin(RTX) for the treatment of patients with idiopathic overactive bladder(IOAB).Methods 26 cases with IOAB were randomly divided into test(A,14 cases) and control(B,12 cases) groups.The patients in group A were treated by intravesical instillation with 40ml of 0.5% idocain retained within the bladder for 3 minutes at first and with 100ml of 100nmol/L RTX retained within the bladder for 30 minutes late.The patients in group B were treated by the same method,however,the furacilin solution(placebo) at a dilution 1∶5000 was used instead of 100nmol/L RTX.The efficacy(daily voiding frequency,urgent uresis,FDV,MCBC,Qmax) of the 2 groups were evaluated before,1 month and 3 months after treatment.Results The pre-treatment comparion with prost-treatmen at 1 month and 3 months of A group was significant(P0.05).14 patients(54%) had slightly stimulating symptom in the urethra or bladder,and were otherwise generally well tolerated.Conclusion Single administration of RTX is safe and effective in patients with IOAB.

Purpose Synchronous multiple primary esophageal carcinomas may be undetected by gastroscopy. This study aims to assess the role of 18F-fluorodeoxyglucose (18F-FDG) PET/CT in detecting synchronous multiple primary esophageal carcinomas. Materials and Methods Thirteen patients with synchronous multiple primary esophageal carcinoma who underwent gastroscopy and 18F-FDG PET/CT within two weeks before treatment were retrospectively analyzed. Results Gastroscopy and 18F-FDG PET/CT detected 22 and 29 esophageal carcinomatous foci respectively, and the difference was statistically significant (Z=-2.333, P<0.05). All the 29 esophageal carcinomatous foci showed high 18F-FDG uptake with a SUVmax ranging from 3.1 to 20.1 (mean, 10.7±5.7). 18F-FDG PET/CT also detected surrounding organ invasion in 3 patients, lymphatic metastasis in 10 patients and distant metastasis in 3 patients. Conclusion 18F-FDG PET/CT detects more esophageal carcinomatous foci compared with gastroscopy in patients with synchronous multiple primary esophageal carcinomas and also performs the staging diagnosis, which may help in improving clinical management and prognosis.

In order to improve the teaching quality of seven-year program courses,and by adopting LBL,PBL and CBL,we develop a comprehensive teaching mode helpful to the develop-ment of students.This teaching mode,which has better teaching effect than the traditional one,can pay attention to teaching purposes,excitate the students'learning potential,lead them to study more independently.