Objective To explore the psychological intervention combined with intracapsular injection of kidney fat in the treatment of nephropathy clinical analysis, looking for effective treatment.Methods 140 patients with nephropathy were enrolled in our hospital from March 2014 to September 2016.Patients were randomly divided into study group(n=70) and control group(n=70) according to the international character table method.Group of patients with a single renal fat capsule drug injection therapy, the study group to be combined with psychological intervention + kidney fat intracapsular injection of drug treatment, the treatment cycle after the end of two groups of patients compared with 24 hours of urine protein quantification, urine microalbumin;The incidence of adverse events in the two groups of patients was compared between the two groups of patients with emotional status(SAS, SDS scale).Results The levels of urinary albumin in the study group were(139.75±20.04) μg/min, and the 24h urine protein level was(1.43±0.59) g/24h significantly lower than the control group(189.74±19.54) μg / min,(4.77±2.14) g / 24h(P<0.05).The incidence of adverse reactions in the study group was 4.29%, which was lower than that of the control group(7.14%).However, there was no significant difference between the two groups.The self-rating scale of the study group(32.35±2.19)(38.65±3.54) points were lower than the control group(55.97±2.14) points,(54.21±7.89) points(P<0.05).Conclusion Psychological intervention combined with intracapsular injection of renal capsule can significantly improve the renal function of patients, can interfere with the patient's bad mood, and high security, health care workers can be promoted.

[Objective] A randomized control ed trial of traditional Chinese medicine recipe Gujing Tang treatment of premature ejaculation was studied. [Methods] The subjects: 60 patients with premature ejaculation form department of andrologic outpatient, the patients were randomly divided into two groups, Integrated TCM-WM Groups and paroxetine Groups. 30 cases in each group. The Integrated TCM-WM Groups were given Gujing Tang , with paroxetine 10mg, oral, Qd at the same time; The paroxetine groups were given paroxetine 10mg, oral, Qd. Both groups were treated for 3 months. Ejaculation status was evaluated at the timepoint:before treatment, 3 months after treatment,and fol ow-up evaluation of six months after treatment. Evalu-ate parameter including Intravaginal ejaculation latency(IELT) and score of sexual function and Chinese patients with premature ejaculation evaluation -5 (CIPE-5). [Results] After 3 months, both groups were significantly improved, and Integrated TCM-WM Groups improved more significantly. After 6 months of fol ow-up, IELT, motion times and CIPE-5 score of Integrated TCM-WM Groups decreased slightly, but stil had significant difference com-pared to before treatment, and this parameter of Paroxetine groups was returned to the level of baseline, and had no significant difference compared with baseline. [Conclusion] Traditional Chinese medicine Recipe Gujing Tang combined with paroxetine, could not only have rapid onset, enhance patient confidence, improve the psychological state, but also improve the long-term efficacy and avoid recurrent disease after treatment, therefore can achieve ef-fective treatment of premature ejaculation.

Objective To explore the effect of Schisandrae Sphenantherae on proliferation and differentiation of rat osteoblast in vitro. Methods Using the method of serum pharmacology, osteoblast was isolated from calvaria of newborn SD rats by means of modified sequential collagenase digestion and incubated in RPMI 1640 medium. The cell morphology was observed under a phase contrast inverted microscope. MTT assay, ALP activity, mineral node count were detected to determine the status and activities of proliferation and differentiation. Results In 10%, 15% concentration groups after cultured 48 h and 5%, 10% after cultured 72 h, 75% ethanol extracts of Schisandrae Sphenantherae significantly stimulated the proliferation (P

Objective To investigate the effects of ultra-filtration extract from the mixture of Angelicae Sinensis Radix and Hedysarum Polybotrys (UFE-AH) on the expressions of HSP70 and eNOS in H2O2-induced endothelial cell apoptosis. Methods H2O2 induced ECV-304 cell apoptosis to prepare models. The experiment was divided into normal control group, model group, simple medicine group, medicine intervention group, and all treatment groups received relevant medicine for intervention. Flow cytometry (FCM) was used to detect apoptosis and concentration of intracellular Ca2+;RT-PCR was used to detect the mRNA expression of HSP70 and eNOS;Western blot was used to detect the expression of HSP70 protein;Nitrale reduetase and spectrophotometric method were employed to detect the content of NO. Results Compared with normal control group, cell apoptosis rate, concentration of intracellular Ca2+, and expression of HSP70 increased significantly in model group (P<0.05);gene expression of eNOS mRNA and content of NO decreased (P<0.05). Compared with model group, cell apoptosis rate and concentration of intracellular Ca2+dropped in medicine intervention group (P<0.05);expressions of HSP70, eNOS mRNA and content of NO increased (P<0.05). Conclusion UFE-AH can confront H2O2-induced cell apoptosis H2O2 of ECV-304 human umbilical vein endothelial by increasing the expressions of HSP70 and eNOS and content of NO, and reducing the intracellular calcium overload.

Objective To investigate cause,diagnosis,treatment and prevention of vertebral artery injury in cervical spine surgery.Methods Data of 7 patients with vertebral artery injury caused by cervical spine surgery from October 2002 to April 2012 were retrospectively analyzed.There were 6 males and 1 female,aged from 23 to 65 years (average,48.9 years).The reasons of cervical spine surgery were as follows:cervical spondylotic myelopathy (5 cases),traumatic subluxation of C4 and C5 (1 case),and cervical spinal stenosis due to skeletal fluorosis (1 case).All cases had unilateral vertebral artery injury,including 4 cases in the left side and 3 cases in the right side.The cause,treatment and prognosis of vertebral artery injury in 7 patients were analyzed.Results Four patients suffered from vertebral artery injury during anterior cervical spine surgery; 2 cases were caused by excessive lateral trepanation,1 by excessive lateral discectomy using a curette,and 1 by Kerrison punch during decompression in a patient with anomalous vertebral artery due to traumatic subluxation of C4 and C5.Three patients suffered from vertebral artery injury during posterior surgery; 2 cases were caused by a drill bit during drilling lateral mass of C4 and 1 by a rongeur during removing posterior C1 arch in a patient with cervical spinal stenosis due to skeletal fluorosis.After vertebral artery injury,all patients were immediately treated by hemostatic tamponade,and then the incisions were closed.However,1 patient occurred delayed cervical hemorrhage 4 weeks after operation.Then he underwent vertebral artery angiography,balloon embolization and evacuation of hematoma.All cases had no cerebral infarction.However,two of them had postoperative temporary dizziness.Conclusion Vertebral artery injury in cervical spine surgery is a rare but serious complication,which is associated with surgical mistakes and anatomical variations.Hemostatic tamponade and embolization are effective for this complication.

Objective To investigate the methods for preventing bladder cancer recurrence after surgical treatment for upper tract urothelial carcinoma.Methods Of the 156 patients who underwent total nephroureterectomy for clinically localized transitional cell carcinoma of the upper urinary trace,139 were followed up and their data were retrospectively analyzed.Of the 139 cases,78 had pyelic carcinoma and 61 had ureteral carcinoma.The tumor cell grading showed G_1 in 19 cases,G_2 in 88 and G_3 in 32.The tumor staging showed T_a-T_1 in 38 cases,T_2 in 80 and T_3-T_4 in 21.The tumor diameter ranged from 0.8 to 6.0 cm.Results Radical nephroureterectomy and resection of partial bladder wall around the ureteral orifice(1.5-2.0 cm) were performed in all the 139 patients;of whom 55(39.6%) experienced subsequent bladder cancer recurrence during a mean follow-up of 52 months(range,1-10 years).The bladder cancer recurrence rate was 18.5%(5/27) in patients whose ureter had been ligated before the kidney was dissociated;and the recurrence rate was 27.5%(14/51) in patients whose ureter had not been firstly ligated.The recurrence rates in those who had prophylactic intravesical instillation immediately after surgery and in those who had the instillation 3 weeks after surgery were 32.3%(10/31) and 34.9%(30/86),respectively.The recurrence rate was 20.0%(4/20) in those who had intravesical instillation on the surgical day and 3 weeks after surgery sequentially,which was significantly lower than that(39.3%,26/66) in those who had the instillation simply 3 weeks after surgery(P

BACKGROUND:Bioactive glass, a multi-phase composite material, has good biological activity, bone conductivity and biocompatibility, but as a bone repair material it cannot be completely degraded, and has low mechanical strength that is insufficient.
OBJECTIVE:To design a kind of bioactive glasses/chitosan composite scaffold, and to investigate its physicochemical properties and cellcompatibility.
METHODS:Hydrochloric acid solution containing 2.0%chitosan was mixed withβ-glycerophosphate at a radio of 7:1 to prepare chitosan solution. Bioactive glasses of 0.5, 1.0, 1.5 g were added into the prepared chitosan solution, and the mass ratios of chitosan and bioactive glass were 2:1, 1:1, and 1:1.5 respectively. The composite materials were immersed and mineralized in simulated body fluid for 7 days.
RESULTS AND CONCLUSION:Scanning electron microscopy showed that the composite scaffold had an interconnected porous structure with the porosity of 89%and the pore size of 100-300μm;bioactive glasses dispersed in a needle shape between the chitosan scaffolds, arranged evenly, and were ful y wrapped tightly by the scaffolds. With the increase in mass of bioactive glass, the porosity of the composites decreased, but the fracture strength gradual y increased. There was a positive correlation between the composite porosity and fracture strength. X-ray diffraction and Fourier transform infrared spectroscopy confirmed that the composite scaffold appeared to have no changes in the nature of single materials, and differential scanning calorimetry analysis showed no mass loss at normal body temperature. After 3 days of mineralization, hydroxyapatite forming on the material surface gradual y grew up as a vil ous shape, and also significantly increased in number. After 7 days of mineralization, hydroxyapatite changed from a vil ous shape to a needle shape, the amount of hydroxyapatite was increased further, and many mineralized products were in a spherical shape.

This study was designed to investigate the impact of ultra-filtration extract mixture from Astragals mongholicus (UEMAM) o radiosensitivity of H22 ascitic tumor in mice to 12C6+ ions radiation. The H22 ascitic tumor model was established in mice by intraperitoneal injection of 0.2 mL H22 ascitic cells. The animals were subsequently divided into 4 groups randomly, treated with normal saline, UEMAM, heavy ion beam radiotherapy and UEMAM plus heavy ion beam radiotherapy, respectively. The body weights, abdomen circumference of the mice were measured and the mouse behavior was monitored every day; survival time was recorded to evaluate life extension effect; flow cytometry technique was used to detect H22 cell apoptosis and cell cycle; protein levels of p53, Bax, Bcl-2 and cleaved Caspase-3 were analyzed by Western blot; the single cell gel electrophoresis was used to detect the level of deoxyribonucleic acid damage (DNA damage). The results suggest that UEMAM significantly increased survival time, and decreased body weights and abdomen circumference over the saline control group. The treatment increased cell apoptosis, cycle arrest and DNA damage compared to the saline control group. UEMAM significantly enhanced the therapeutic effect of heavy ion beam radiation in survival time, and decreased body weights and abdomen circumference in the tumor-baring mice. The combination increased cell apoptosis, cycle arrest and DNA damage compared to the radiotherapy group. The results of Western blot suggest that the treatment significantly enhanced p53-induced apoptotic signals. The experiment discovered that UEMAM could improve radiosensitivity of H22 ascitic tumor through activation of p53-mediated apoptotic signal pathway.

Objective:To explore the effect of Angelica Sinensis Volatile Oil on the mi RNA expression profiling in myocardium tissue of the spontaneously hypertensive rats (SHRs),and to clarify the possible mechanism of Angelica Sinensis Volatile Oil.Methods:All the SHRs were divided into Angelica group,model group and captopril group,and other Wistar rats with the same age were selected as control group.The non-invasive systolic blood pressure was detected;after 4 weeks of administration,the changes of miRNA expression profiling in myocardium tissue were measured by Affymetrix miRNA 4.0 Array.KEGG analysis was used to identify the target genes. Results:Compared with control group,the systolic blood pressure of the rats in model group was significantly decreased (P < 0.05)before treatment.4 weeks after administration,compared with model group,the systolic blood pressure of the rats in Angelica group was significantly decreased (P <0.05).Compared with model group, 29 differential miRNAs of rats in Angelica group were found (P < 0.05 ), with 13 up-regulated miRNAs and 16 down-regulated miRNAs.The KEGG analysis results showed that miR-19a,let-7i,and miR-181c were related to insulin signaling pathways; let-7i, miR-181a, and miR-455 were related to VEGF signaling pathways;miR-122,miR-181a, miR-200b, miR-181c, let-7i, and miR-19a were related to apoptosis (P < 0.05 ). Conclusion:Angelica Sinensis Volatile Oil can decrease the blood pressure in SHRs and it can regulate the blood pressure by regulating the miRNA related to insulin signaling pathways and VEGF signaling pathways.

Objective:To retrospectively analyze the clinical data of the patients with reoperation for cervical myelopathy due to progressing ossification of the posterior longitudinal ligaments,with previous open-door expansive laminoplasty,and to evaluate the outcomes.Methods:From May 2006 to July 2012,a retrospective study was performed on a consecutive series of 17 patients with previous open-door expansive laminoplasty,who had received the reoperation for cervical myelopathy due to progressing ossi-fication of the posterior longitudinal ligaments.The reoperation was performed based on the clinical mani-festations and segments of responsibility.The anterior approaches were performed in 12 cases,and the posterior approaches in 5 cases.The correlation between the clinical factors and Japanese Orthopedic As-sociation (JOA)scores or the JOA recovery rate was evaluated by Pearson or Spearman correlation test. The pre-and post-operative JOA scores were analyzed by repeated measures ANOVA and the JOA recovery rates were compared with paired t test.Results:The mean follow-up was 137.5 months (range 60-348 months).There were no serious complications after surgical procedures.There was one case that had C5 palsy in the first operation and had recovery after one week.Another case had C5 palsy in the reoperation with posterior approach,which had recovery at the end of 6 months post-operation.Three cases had the cerebrospinal fluid leakage of the reoperation,with two cases in the anterior approaches and one case in the posterior approach.There was no significant correlation between the clinical variables and JOA scores or JOA recovery rates.The JOA scores of the patients in the first operation were improved from 9.4 ±4.1 to 12.8 ±2.8 (P<0.01),and the JOA recovery rate was 45.6%.The JOA scores of the reoperation were improved from 10.2 ±2.8 to 12.7 ±2.4 (P<0.05)at the end of 6 months and 14.3 ±1.9 (P<0.01)by the last follow-up.There were significant differences between the JOA recovery rates by the last follow-up (63 .2%)and at the end of 6 months (39 .3%)of the reoperation or 45 .6%of the first opera-tion (P<0.01).Conclusion:The reoperation for cervical myelopathy duo to progressing ossification of the posterior longitudinal ligaments can significantly promote the recovery of the spinal cord,based on the clinical manifestations combined with segments of responsibility of the imaging.